Patent EP0047600A2 successfully refreshed from Google Patents.
EP0047600A2
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EP
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Bovine pre-growth and growth hormone
Assignee: University of California
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Filing: 1981-08-21
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Granted: —
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Expires: 2001-08-21
Abstract
Abstract A DNA transfer vector comprises a deoxynucleotide sequence coding for bovine growth hormone or bovine pre-growth hormone.
Inventors
- 👤 Walter L. Miller
- 👤 Joseph A. Martial
- 👤 John D. Baxter
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-25 23:33
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/70; C12N15/71; C07; C07K; C07K14/00; C07K14/435; C07K14/575; C07K14/61; C07K2319/00; C07K2319/01; C07K2319/02; C07K2319/70; C07K2319/74; C07K2319/75
Claims Analysis
Total Claims: 32
Claim 1
Independent Claim
13 words
A DNA transfer vector comprising a deoxynucleotide sequence coding for bovine pre-growth hormone.
Claim 2
Dependent Claim
13 words
A DNA transfer vector comprising a deoxynucleotide sequence coding for bovine pre-growth hormone.
Claim 3
Dependent Claim
13 words
A DNA transfer vector comprising a deoxynucleotide sequence coding for bovine growth hormone.
Claim 4
Dependent Claim
13 words
A DNA transfer vector comprising a deoxynucleotide sequence coding for bovine growth hormone.
Claim 5
Dependent Claim
32 words
The DNA transfer vector of claim 1 wherein said deoxynucleotide sequence comprises a plus strand having the sequence: wherein A is deoxyadenyl, G is deoxyguanyl, C is deoxycytosyl and T is thymidyl.
Claim 6
Dependent Claim
32 words
The DNA transfer vector of claim 2 wherein said deoxynucleotide sequence comprises a plus strand having the sequence: wherein A is deoxyadenyl, G is deoxyguanyl, C is deoxycytosyl and T is thymidyl.
Claim 7
Dependent Claim
32 words
The DNA transfer vector of claim 2 wherein said deoxynucleotide sequence comprises a plus strand having the sequence: wherein A is deoxyadenyl, G is deoxyguanyl, C is deoxycytosyl and T is thymidyl.
Claim 8
Dependent Claim
17 words
The DNA transfer vector of claim 1 or 3 wherein said transfer vector is the plasmid pBR348.
Claim 9
Dependent Claim
14 words
A microorganism transformed by the transfer vector of any of claims 1 to 5.
Claim 10
Dependent Claim
11 words
The microorganism of claim 7 wherein the microorganism is Escherichia coli.
Claim 11
Dependent Claim
20 words
The microorganism of claim 8 wherein the microorganism is selected from the group comprising Escherichia coli χ1776, HB101 or RRI.
Claim 12
Dependent Claim
27 words
A fusion protein comprising the amino acid sequence of bovine pre-growth hormone as its C-terminal end and a portion of a procaryotic protein as its N-terminal end.
Claim 13
Dependent Claim
27 words
A fusion protein comprising the amino acid sequence of bovine pre-growth hormone as its C-terminal end and a portion of a procaryotic protein as its N-terminal end.
Claim 14
Dependent Claim
28 words
.11. A fusion protein comprising the amino acid sequence of bovine growth hormone as its C-terminal end and a portion of a procaryotic protein as its N-terminal end.
Claim 15
Dependent Claim
28 words
.11. A fusion protein comprising the amino acid sequence of bovine growth hormone as its C-terminal end and a portion of a procaryotic protein as its N-terminal end.
Claim 16
Dependent Claim
53 words
A process for preparing a deoxynucleotide sequence coding for bovine growth hormone comprising cleaving a cDNA coding for bovine pre-growth hormone with the restriction enzyme Hae II and incubating the cleaved product with an enzyme selected from the group comprising the Klenow fragment of DNA polymerase I, T4 DNA polymerase or 3'-5' exonuclease.
Claim 17
Dependent Claim
53 words
A process for preparing a deoxynucleotide sequence coding for bovine growth hormone comprising cleaving a cDNA coding for bovine pre-growth hormone with the restriction enzyme Hae II and incubating the cleaved product with an enzyme selected from the group comprising the Klenow fragment of DNA polymerase I, T4 DNA polymerase or 3'-5' exonuclease.
Claim 18
Dependent Claim
43 words
The process of claim 12 wherein the incubation with said Klenow fragment is conducted in the presence of dATP and the resulting product = digested with Sl nuclease whereby a deoxynucleotide sequence coding for amino acids 2-191 of bovine growth hormone is obtained.
Claim 19
Dependent Claim
73 words
The process of claim 12 wherein the incubation with said Klenow fragment is conducted in the presence of dATP, dGTP, dCTP, dTTP, the resulting product is incubated with an enzyme selected from the group comprising said Klenow fragment, T4 DNA polymerase or 3'-5' exonuclease in the presence of dCTP and the resulting product is digested with Sl nuclease whereby a deoxynucleotide sequence coding for amino acids 1-191 of bovine growth hormone is obtained.
Claim 20
Dependent Claim
47 words
A process for making a DNA transfer vector for use in maintaining and replicating a deoxynucleotide sequence coding for bovine pre-growth hormone, characterized by reacting a deoxynucleotide sequence coding for bovine growth hormone with a DNA molecule prepared by cleaving a transfer vector with a restriction enzyme.
Claim 21
Dependent Claim
47 words
A process for making a DNA transfer vector for use in maintaining and replicating a deoxynucleotide sequence coding for bovine pre-growth hormone, characterized by reacting a deoxynucleotide sequence coding for bovine growth hormone with a DNA molecule prepared by cleaving a transfer vector with a restriction enzyme.
Claim 22
Dependent Claim
37 words
The process of claim 15 characterized in that the deoxynucleotide sequence coding for bovine pre-growth hormone comprises a plus strand having the sequence: wherein A is deoxyadenyl, G is deoxyguanyl, C is deoxycytosyl and T is thymidyl.
Claim 23
Dependent Claim
71 words
A process for preparing a deoxynucleotide sequence coding for bovine growth hormone for use in synthesizing bovine growth hormone characterized by cleaving a cDNA coding for bovine pre-growth hormone, maintained and replicated according to the process of claim 15, with the restriction enzyme Hae II and incubating the cleaved product with an enzyme selected from the group comprising the Klenow fragment of DNA polymerase I, T4 DNA polymerase or 3'-5' exonuclease.
Claim 24
Dependent Claim
44 words
The process of claim 17 characterized in that the incubation with said Klenow fragment is conducted in.the presence of dATP and the resulting product is digested with Sl nuclease whereby a deoxynucleotide sequence coding for amino acids 2-191 of bovine growth hormone is obtained.
Claim 25
Dependent Claim
37 words
The process of claim 18 characterized in that the deoxynucleotide sequence coding for bovine growth hormone comprises a plus strand having the sequence: wherein A is deoxyadenyl, G is deoxyguanyl, C is deoxycytosyl and T is thymidyl.
Claim 26
Dependent Claim
66 words
The process of claim 17 characterized in that the incubation with said Klenow fragment is conducted in the presence of dATP, dGTP, dCTP and dTTP the resulting group comprising said Klenow fragment, T4 DNA polymerase or 3'-5' exonuclease in the presence of dCTP and digesting the resulting product with Sl nuclease whereby a deoxynucleotide sequence coding for amino acids 1-191 of bovine growth hormone is obtained.
Claim 27
Dependent Claim
37 words
The process of claim 20 characterized in that the deoxynucleotide sequence coding for bovine growth hormone comprises a plus strand having the sequence: wherein A is deoxyadenyl, G is deoxyguanyl, C is deoxycytosyl and T is thymidyl.
Claim 28
Dependent Claim
38 words
A process of making the plasmid pBP348 according to claim 15 or 16 characterized in that the DNA molecule prepared by cleaving a transfer vector with a restriction enzyme is pBR322 and the restriction enzyme is Pst I.
Claim 29
Dependent Claim
57 words
A process for making a DNA transfer vector for use in maintaining and replicating a deoxynucleotide sequence coding for bovine growth hormone characterized by reacting a deoxynucleotide sequence coding for bovine growth hormone, made according to any one of claims 17 to 21, with a DNA molecule prepared by cleaving a transfer vector with a restriction enzyme.
Claim 30
Dependent Claim
58 words
A process for making an expression transfer vector in accordance with claim 15, 16, or 23 for use in expressing a deoxynucleotide sequence coding for bovine pre-growth hormone or bovine growth hormone characterized in that the DNA molecule- prepared by cleaving a transfer vector with a restriction enzyme is cleaved at a point within an expressible control region.
Claim 31
Dependent Claim
73 words
A process for making a fusion protein comprising the amino acid sequence of bovine pre-growth hormone or the amino acid sequence of bovine growth hormone as its C-terminal end and a portion of a procaryotic protein as its N-terminal end characterized by incubating a microorganism transformed by an expression transfer vector comprising a deoxynucleotide sequence coding for said bovine pre-growth hormone or for said bovine growth hormone prepared in accordance with claim 24.
Claim 32
Dependent Claim
65 words
A process for synthesizing bovine growth hormone characterized by incubating a microorganism transformed by an expression transfer vector comprising a deoxynucleotide sequence coding for said bovine growth hormone prepared in accordance with any of claims 17 to 21 under conditions suitable for expression of said sequence coding for bovine growth hormone and purifying bovine growth hormone from the lysate or culture medium of said microorganism.
References Cited (Backward Citations)
No citation records indexed.
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