EP0045334A1
Expired / Lapsed
EP
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Propranolol antigen conjugates and antibodies and method of using them
Assignee: Syva Co
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Filing: 1980-08-04
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Granted: —
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Expires: 2000-08-04
Abstract
Abstract A compound of the Formula wherein: R is an aliphatic linking group of from 1 to 8 carbon atoms and 0 to 4 heteroatoms which are oxygen, nitrogen or sulfur, having from 0 to 1 site of ethylenic unsaturation as the only aliphatic unsaturation, any nitrogen being present as tertiary amino or amido and any sulfur being present as thio; X and Z are taken together to form oxo or imino or can each be hydrogen; A is hydrogen, hydroxy, alkoxy of from 1 to 6 carbon atoms, or an antigenic poly(amino acid) or polysaccharide, or an enzyme; n is on the average from 1 to the molecular weight of A divided by 1000, with the proviso that n is 1 when A is hydrogen, hydroxy or alkoxy, is, or is a percursor of, conjugates used in the detection of β-adrenergic blocking agents; antigenic conjugates are employed for the preparation of antibodies and enzyme conjugates as detectors. A detection method using the latter is also claimed.
Inventors
- 👤 Prithipal Singh
- 👤 Marcel Rene Pirio
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-31 19:34
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/94; G01N33/9453
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
128 words
A compound of the formula: wherein: R is an aliphatic linking group of from 1 to 8 carbon atoms and 0 to 4 heteroatoms which are oxygen, nitrogen or sulfur, having from 0 to 1 site of ethylenic unsaturation as the only aliphatic unsaturation, any nitrogen being present as tertiary amino or amido and any sulfur being present as thio; X and Z are taken together to form oxo or imino or can each be hydrogen; A is hydrogen, hydroxy, alkoxy of from 1 to 6 carbon atoms, or an antigenic poly(amino acid) or polysaccharide, or an enzyme; n is on the average from 1 to the molecular weight of A divided by 1000, with the proviso that n is 1 when A is hydrogen, hydroxy or alkoxy.
Claim 2
Dependent Claim
128 words
A compound of the formula: wherein: R is an aliphatic linking group of from 1 to 8 carbon atoms and 0 to 4 heteroatoms which are oxygen, nitrogen or sulfur, having from 0 to 1 site of ethylenic unsaturation as the only aliphatic unsaturation, any nitrogen being present as tertiary amino or amido and any sulfur being present as thio; X and Z are taken together to form oxo or imino or can each be hydrogen; A is hydrogen, hydroxy, alkoxy of from 1 to 6 carbon atoms, or an antigenic poly(amino acid) or polysaccharide, or an enzyme; n is on the average from 1 to the molecular weight of A divided by 1000, with the proviso that n is 1 when A is hydrogen, hydroxy or alkoxy.
Claim 3
Dependent Claim
14 words
A compound according to Claim 1 wherein the carbinol is D, L or racemic.
Claim 4
Dependent Claim
13 words
A compound according to Claim 2 wherein A is an antigenic poly(amino acid).
Claim 5
Dependent Claim
11 words
A compound according to Claim 2 wherein A is an enzyme.
Claim 6
Dependent Claim
47 words
An antibody prepared in immunological response to an antigen conjugate of a β-adrenergic blocking agent according to Claim 1, and which has sites common to and capable of binding to said β-adrenergic blocking agent and an enzyme conjugate of said β-adrenergic blocking agent according to Claim 4.
Claim 7
Dependent Claim
118 words
A method for determining the presence of a drug which is a β-adrenergic blocking agent in a medium suspected of containing said drug which comprises: forming, at a pH in the range of about 5-10 and at a temperature of from about 10°C - 50°C, an aqueous solution of said medium, an enzyme conjugate of said S-adrenergic blocking agent according to Claim 4, and antibody having sites common to and capable of binding to said drug and said enzyme conjugate, said antibody being at a sufficient concentration to substantially reduce the enzymatic activity of said enzyme conjugate in the absence of said drug; and analyzing said solution for the enzymatic activity of said enzyme conjugate in said solution.
Claim 8
Dependent Claim
102 words
A compound of the formula: wherein: R is aliphatic linking group of from 1 to 8 carbon atoms and 0 to 4 heteroatoms which are oxygen, nitrogen or sulfur, having from 0 to 1 site of ethylenic unsaturation as the only aliphatic unsaturation, any nitrogen being present as tertiary amino or amido and any sulfur being present as thio; X and Z are taken together to form oxo or imino or can each be hydrogen; A is an antigenic poly(amino acid) or polysaccharide, or an enzyme; n is on the average from 1 to the molecular weight of A divided by 1000.
Claim 9
Dependent Claim
102 words
A compound of the formula: wherein: R is aliphatic linking group of from 1 to 8 carbon atoms and 0 to 4 heteroatoms which are oxygen, nitrogen or sulfur, having from 0 to 1 site of ethylenic unsaturation as the only aliphatic unsaturation, any nitrogen being present as tertiary amino or amido and any sulfur being present as thio; X and Z are taken together to form oxo or imino or can each be hydrogen; A is an antigenic poly(amino acid) or polysaccharide, or an enzyme; n is on the average from 1 to the molecular weight of A divided by 1000.
Claim 10
Dependent Claim
15 words
A compound according to Claim 1 wherein the carbinol is D or L or racemic.
Claim 11
Dependent Claim
13 words
A compound according to Claim 2 wherein A is an antigenic poly(amino acid).
Claim 12
Dependent Claim
11 words
A compound according to Claim 2 wherein A is an enzyme.
Claim 13
Dependent Claim
46 words
An antibody prepared in immunological response to an antigen conjugate of a β-adrenergic blocking agent according to Claim 1,.and which has sites common to and capable of binding to said β-adrenergic blocking agent and an enzyme conjugate of said β-adrenergic blocking agent according to Claim 4.
Claim 14
Dependent Claim
118 words
A method for determining the presence of a drug which is a β-adrenergic blocking agent in a medium suspected of containing said drug which comprises: forming, at a pH in the range of about 5-10 and at a temperature of from about 10°C - 50°C, an aqueous solution of said medium, an enzyme conjugate of said β-adrenergic blocking agent according to Claim 4, and antibody having sites common to and capable of binding to said drug and said enzyme conjugate, said antibody being at a sufficient concentration to substantially reduce the enzymatic activity of said enzyme conjugate in the absence of said drug; and analyzing said solution for the enzymatic activity of said enzyme conjugate in said solution.
References Cited (Backward Citations)
GB1245219A
Cited
US4070492A
Cited
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