Patent EP0042755A2 successfully refreshed from Google Patents.
EP0042755A2
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EP
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Processes and apparatus for carrying out specific binding assays
Assignee: Unilever PLC
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Filing: 1981-06-22
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Granted: —
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Expires: 2001-06-22
Abstract
Abstract A process for carrying out a specific binding assay (for example an immunoassay) in which (a) a sample under assay, possibly containing a substance being tested for, is reacted with (b) a specific binding partner for the substance being tested for, immobilised on a solid support, and (c) a specific binding partner for the substance being tested for which is conjugated to a detectable marker, thereby to form a complex by reaction between whatever quantities are present of the substance being tested for with reagents (b) and (c), in which the marker is immobilised to the support via the substance being tested for, andis detected or assayed as an index of the quantity present in the sample (a) of any of the substance being tested for; characterised in that reaction ingredients (a), (b) and (c) are all mixed in a single step for reaction in a single reaction liquid, and competitive interference between the binding reactions of the substance being tested for and reagents (b) and (c) is avoided either by use of an antibody of narrow and different, non-interfering specificity, such as a monoclonal antibody, to avoid the interference, or by use of a slow-release form of reagent (c).
Inventors
- 👤 Paul James Davis
- 👤 Philip Porter
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-31 18:35
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/577; G01N33/543; G01N33/54366; G01N33/54393
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
201 words
A process for carrying out a specific binding assay (for example an immunoassay) in which (a) a sample under assay, possibly containing a substance being tested for, is reacted with (b) a specific binding partner for the substance being tested for, immobilised on a solid support, and (c) a specific binding partner for the substance being tested for which is conjugated to a detectable marker, thereby to form a complex by reaction between whatever quantities are present of the substance being tested for with reagents (b) and (c), in which the marker is immobilised to the support via the substance being tested for, and is detected or assayed as an index of the quantity present in the sample (a) of any of the substance being tested for; characterised in that reaction ingredients (a), (b) and (c) are all mixed in a single stop for reaction in a single reaction liquid, and competitive interference between the binding reactions of the substance being tested for and reagents (b) and (c) is avoided either by use of an antibody of narrow and different, non-interfering specificity, such as a monoclonal antibody, to avoid the interference, or by use of a slow-release form of reagent (c).
Claim 2
Dependent Claim
201 words
A process for carrying out a specific binding assay (for example an immunoassay) in which (a) a sample under assay, possibly containing a substance being tested for, is reacted with (b) a specific binding partner for the substance being tested for, immobilised on a solid support, and (c) a specific binding partner for the substance being tested for which is conjugated to a detectable marker, thereby to form a complex by reaction between whatever quantities are present of the substance being tested for with reagents (b) and (c), in which the marker is immobilised to the support via the substance being tested for, and is detected or assayed as an index of the quantity present in the sample (a) of any of the substance being tested for; characterised in that reaction ingredients (a), (b) and (c) are all mixed in a single stop for reaction in a single reaction liquid, and competitive interference between the binding reactions of the substance being tested for and reagents (b) and (c) is avoided either by use of an antibody of narrow and different, non-interfering specificity, such as a monoclonal antibody, to avoid the interference, or by use of a slow-release form of reagent (c).
Claim 3
Dependent Claim
26 words
A process according to claim 1, characterised in that a monoclonal antibody immobilised on a solid surface to form an immunosorbent is comprised in reagent (b).
Claim 4
Dependent Claim
22 words
A process according to claim 1 or 2, characterised in that a monoclonal antibody in marker-conjugated form is .comprised in reagent (c).
Claim 5
Dependent Claim
35 words
A process according to claim 1,2 or 2, characterised in that reagent (c) is used in the reaction in slow-release form such as a glaze coated on a surface in contact with the reaction liquid.
Claim 6
Dependent Claim
51 words
A process according to any of claims 1-4, characterised in that the specific binding reagent (b) is immobilised on the surface of a displacer body (2) which occupies a majority of the volume of a well or cup (1) containing the aqueous liquid in which the specific binding reaction takes place.
Claim 7
Dependent Claim
52 words
A process according to claim 5, in which the displacer body (2) has a shape substantially complementary to and slightly smaller than that of the well or cup (1), preferably reducing the volume available in the well or cup (1) for occupation by the aqueous reaction liquid by a factor of a-10.
Claim 8
Dependent Claim
61 words
A process according to any of claims 1-6, characterised in that the immobilised specific binding reagent (b) is either an immobilised antibody or an immobilised substance to which an immunoglobulin under assay can specifically bind, and marker conjugated-reagent (c) comprises either another antibody specific to the binding partner of antibody reagent (b), or an antiglobulin specific for an immunoglobulin under assay.
Claim 9
Dependent Claim
109 words
A process for carrying out a specific binding assay in which (a) a sample under assay, possibly containing a substance being tested for, is reacted with (b) an antibody of corresponding specificity immobilised on a solid support and (c) an antibody (also with binding specificity for the substance being tested for) conjugated to a detectable marker, thereby to form a complex between whatever quantities are present of the substance being tested for with reagents (b) and (c), and in which after the binding reaction the amount of detectable marker bound is directly or indirectly assayed, characterised in that the antibodies comprised in reagents (b) and (c) are monoclonal antibodies.
Claim 10
Dependent Claim
109 words
A process for carrying out a specific binding assay in which (a) a sample under assay, possibly containing a substance being tested for, is reacted with (b) an antibody of corresponding specificity immobilised on a solid support and (c) an antibody (also with binding specificity for the substance being tested for) conjugated to a detectable marker, thereby to form a complex between whatever quantities are present of the substance being tested for with reagents (b) and (c), and in which after the binding reaction the amount of detectable marker bound is directly or indirectly assayed, characterised in that the antibodies comprised in reagents (b) and (c) are monoclonal antibodies.
Claim 11
Dependent Claim
104 words
Materials for carrying out specific binding assay according to any of claims 1-8, comprising (i) an immobilised specific binding partner for a substance to be tested for, carried on a solid support, and (ii) a marker-conjugated specific binding partner for the substance to be tested for, which can be added to a reaction liquid contacting immobilised reagent (i) either as a slow-release form, or in any form provided that the specific binding partners in reagents (i) and (ii) include an antibody of narrow specificity so that reagents (i) and (ii) do not interfere with each other's binding reactions with the substance to be tested.
Claim 12
Dependent Claim
27 words
Processes and materials for carrying out specific binding assay, substantially as described above with reference to the joint and several features of the accompanying specification and drawings.
Claim 13
Dependent Claim
27 words
Processes and materials for carrying out specific binding assay, substantially as described above with reference to the joint and several features of the accompanying specification and drawings.
Claim 14
Dependent Claim
46 words
A process for carrying out immunoassay in which a sample under assay is reacted with two specific binding reagents of which one or each is a monoclonal antibody of narrow and different, non-interfering specificity to ensure that the desired assay reactions do not impede each other.
Claim 15
Dependent Claim
46 words
A process for carrying out immunoassay in which a sample under assay is reacted with two specific binding reagents of which one or each is a monoclonal antibody of narrow and different, non-interfering specificity to ensure that the desired assay reactions do not impede each other.
References Cited (Backward Citations)
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