Patent US2610989A successfully refreshed from Google Patents.
Patent EP0023397A1 successfully refreshed from Google Patents.
EP0023397A1
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Heteropolysaccharide; process and microorganism for producing it
Assignee: Assunzione O Variazione Mandato modiano & Associat
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Filing: 1980-07-08
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Granted: —
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Expires: 2000-07-08
Abstract
Abstract Biopolymer PS-87, a heteropolysaccharide comprising glucose, galactose, mannose, glucuronic acid and fucose, is pseudoplastic and has a consistency at 20°C of at least 150 poise and a yield stress value at 20°C of at least 30 dynes/cm 2 . Biopolymer PS-87 is synthesized by a specific strain or mutant of Bacillus polymyxa (NCIB 11429) or by micro-organisms which have undergone genetic manipulations. This heteropolysaccharide has many domestic and industrial applications as a suspending agent or thickener.
Inventors
- 👤 Roger Bertram Cox
- 👤 David Charles Steer
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-05 11:44
CPC Classifications
C; C09; C09K; C09K8/00; C09K8/60; C09K8/84; C09K8/86; C09K8/88; C09K8/90; C09K8/905; A; A23; A23L; A23L29/00; A23L29/20; A23L29/269; A61; A61K; A61K8/00; A61K8/18; A61K8/72; A61K8/73; A61Q; A61Q11/00; C08; C08B; C08B37/00; C08F; C08F2/00; C08F2/12; C08F2/16; C08F2/18; C09D; C09D7/00; C09D7/40; C09D7/43; C09K8/02; C09K8/04; C09K8/14; C09K8/18; C09K8/20; C09K8/206; C11; C11D; C11D3/00; C11D3/16; C11D3/20; C11D3/22; C11D3/222; C12; C12N; C12N1/00; C12N1/20; C12N1/205; C12N15/00; C12N15/09; C12N15/11; C12N15/52; C12P; C12P19/00; C12P19/04; C08L; C08L5/00; C12R; C12R2001/00; C12R2001/01; C12R2001/07; C12R2001/12; C12R2001/185; C12R2001/19; C12R2001/22; C12R2001/38; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/832; Y10S435/838; Y10S507/00; Y10S507/935; Y10S507/936
Claims Analysis
Total Claims: 56
Claim 1
Independent Claim
47 words
Biopolymer PS 87, a heteropolysaccharide comprising glucose, galactose, mannose and glucuronic acid, a 1% by weight solution of Biopolymer PS 87 having pseudoplastic properties, a consistency at 20°C of at least 150 poise and a yield stress value at 20°C of at least 30 dynes/cm 2 .
Claim 2
Dependent Claim
47 words
Biopolymer PS 87, a heteropolysaccharide comprising glucose, galactose, mannose and glucuronic acid, a 1% by weight solution of Biopolymer PS 87 having pseudoplastic properties, a consistency at 20°C of at least 150 poise and a yield stress value at 20°C of at least 30 dynes/cm 2 .
Claim 3
Dependent Claim
10 words
Biopolymer PS 87 according to claim 1 further comprising fucose.
Claim 4
Dependent Claim
47 words
Biopolymer PS 87 according to claim 1 or 2, comprising from 40 to 45% by weight glucose, from 10 to 20% by weight galactose, from 25 to 30% by weight mannose, from 6 to 13% by weight glucuronic acid and from 0 to 1.5% by weight fucose.
Claim 5
Dependent Claim
32 words
Biopolymer PS 87 according to claim 2 or 3, comprising 41% by weight glucose, 12% by weight galactose, 28% by weight mannose, 9% by weight glucuronic acid and 0.8% by weight fucose.
Claim 6
Dependent Claim
19 words
Biopolymer PS 87 according to any preceding claim having a consistency at 20°C of from 200 to 400 poise.
Claim 7
Dependent Claim
23 words
Biopolymer PS 87 according to any preceding claim having a yield stress value at 20°C of from 50 to 100 dynes/cm 2 .
Claim 8
Dependent Claim
17 words
Biopclymer PS 87 according to any preceding claim having a specific rotatory power of = + 60.
Claim 9
Dependent Claim
20 words
Biopolymer PS 87 according to any preceding claim having a molecular weight of 34 + 4 x 10 4 .
Claim 10
Dependent Claim
14 words
A micro-organism capable of synthesising Biopolymer PS 87 as defined in any preceding claim.
Claim 11
Dependent Claim
10 words
A micro-organism according to claim 9 which is Bacillus polymyxa.
Claim 12
Dependent Claim
12 words
A micro-organism according to claim 10 which is Bacillus polymyxa NCIB 11429.
Claim 13
Dependent Claim
49 words
An aerobically cultured micro-organism according to claim 9 which is capable of synthesising Biopolymer PS 87 as a result of genetic transformation or conjugation or modification by plasmids or plasmid hybrids, or phage or phage hybrids, or other vectors, each carrying DNA specifying the synthesis of Biopolymer PS 87.
Claim 14
Dependent Claim
20 words
A micro-organism according to claim 12 produced by the genetic modification of Bacillus sp, E.Coli, Pseudomonas Sp, or Klebsiella Sp.
Claim 15
Dependent Claim
29 words
A method of making genetically modified micro-organisms according to claim 12 or 13 by incorporating into basic micro-organisms genetic information carrying the genes of the.Biopolymer PS 87 synthesis mechanism.
Claim 16
Dependent Claim
102 words
A process for the production of Biopolymer PS 87 according to any of claims 1 to 8, which comprises the steps of: (i) cultivating a strain of a micro-organism containing genetic material specifying Biopolymer PS.87 synthesis under submerged aerobic conditions in an aqueous culture medium comprising a source of carbon, a source of nitrogen, a source of iron, a source of magnesium and a source of phosphorus, the pH of the culture medium being maintained at a value of from 4.5 to 7.5, until substantial formation of Biopolymer PS 87 has occurred, and (ii) isolating Biopolymer PS 87 from the culture medium.
Claim 17
Dependent Claim
61 words
A process according to claim 15 further comprising the steps of: (i) heating the culture medium containing substantial accumulation of Biopolymer PS 87 to a temperature of at least 50°C at a pH value of at least 8, and (ii) subsequently separating coagulated cells and cell debris from the culture medium to provide a visually clear solution of Biopolymer PS 87.
Claim 18
Dependent Claim
30 words
A process according to claim 15 or 16, in which the genetic material is chromosomal DNA or DNA of plasmid or plasmid hybrid or DNA or phage or phage hybrid.
Claim 19
Dependent Claim
19 words
A process according to claim 15,16 or 17, in which the micro-organism is a bacteria of the species Bacillus.
Claim 20
Dependent Claim
14 words
A process according to claim 18, in which the Bacillus species is Bacillus polymyxa.
Claim 21
Dependent Claim
18 words
A process according to claim 19, in which the strain of B. polymyxa is Bacillus polymyxa NCIB 11429.
Claim 22
Dependent Claim
21 words
A process according to any of claims 15 to 20 and substantially as described in any of Examples 1 to 5.
Claim 23
Dependent Claim
15 words
An edible composition comprising Biopolymer PS 87 according to any of claims 1 to 8.
Claim 24
Dependent Claim
19 words
A composition according to claim 22 additionally comprising a tetra-alkali metal phosphate, an edible calcium salt and a gum.
Claim 25
Dependent Claim
18 words
A composition according to claim 22 or 23 and substantially as described in Example 6 or Example 7.
Claim 26
Dependent Claim
30 words
A process for forming a cold milk gel comprising adding to cold milk a composition according to claims 22,23 or 24 and agitating the milk composition to form a gel.
Claim 27
Dependent Claim
15 words
A salad dressing comprising Biopolymer PS 87 according to any of claims 1 to 8.
Claim 28
Dependent Claim
22 words
A salad dressing according to claim 26, further comprising an edible oil, water, an emulsifier, cooked starch, an acidifying ingredient, and salt.
Claim 29
Dependent Claim
16 words
A salad dressing according to claim 26 or 27 and substantially as described in Example 8.
Claim 30
Dependent Claim
77 words
A method of making a spoonable salad dressing according to claim 26,27 or 28 which comprises forming a first mixture containing Biopolymer PS 87 and water; forming a second mixture comprising water together with cooked starch, emulsifier, and salt, blending the first mixture and the second mixture to form a combined first-second mixture with edible oil and with acidifying ingredient until a substantially uniform mixture is obtained; emulsifying the uniform mixture to obtain a spoonable salad dressing.
Claim 31
Dependent Claim
17 words
A dehydrated food product comprising dry Biopolymer PS 87 according to any of claims 1 to 8.
Claim 32
Dependent Claim
28 words
A dehydrated food product according to claim 30, in which Biopolymer PS 87 forms from 0.01 to 1.5% by weight of the hydrated weight of the food product.
Claim 33
Dependent Claim
21 words
A dehydrated food product according to claim 30 or 31 and substantially as described in any of Examples 9 to 13.
Claim 34
Dependent Claim
54 words
A process for preparing a dehydrated food product according to claim 31,32, or 33, which process comprises mixing dry Biopolymer PS 87 with a dry food ingredient, the Biopolymer PS 87 being present in the.food product in an amount of from 0.01 to 1.5% by weight of the hydrated weight of the food product.
Claim 35
Dependent Claim
14 words
A dentifrice comprising Biopolymer PS 87 according to any of claims 1 to 8.
Claim 36
Dependent Claim
103 words
A liquid thixotropic gel dentifrice according to claim 34, that is stable against phase separation of solid abrasive dentifrice particle ingredients which essentially consists of: (i) 25 to 35% by weight of water; (ii) 20 to 35% by weight of a humectant; (iii) 0.5 to 2% by weight of a surfactant; (iv) 0.3 to 2% by weight of Biopolymer PS 87; (v) 20 to 35% by weight of a solid particulate abrasive; whereby said thixotropic gel dentifrice flows easily under low pressure and with good flowability, and when extruded loses its flowability upon removal of extrusion pressure while retaining the shape as extruded.
Claim 37
Dependent Claim
19 words
A dentifrice according to claim 34 or 35 and substantially as described in any of Examples 14,15 or 16.
Claim 38
Dependent Claim
45 words
A water base paint comprising a latex rubber-like water base paint binder, a pigment, an aqueous vehicle for said binder and pigment, and containing Biopolymer PS 87 according to any of claims 1 to 8, in an amount sufficient to give the paint pseudoplastic properties.
Claim 39
Dependent Claim
32 words
A water base paint according to claim 37, in which Biopolymer PS 87 is present in an amount of from 0.01 to 8% by weight of the total weight of said paint.
Claim 40
Dependent Claim
34 words
A water base paint according to claim 37 or 38, in which Biopolymer PS 87 is present in an amount of from 0.2 to 0.7% by weight of the total weight of said paint.
Claim 41
Dependent Claim
31 words
A water base paint according to claim 37,38 or 39, in which the binder is chosen from a synthetic latex, a polyvinyl acetate emulsion, an acrylic emulsion, and a styrene-butadiene emulsion.
Claim 42
Dependent Claim
19 words
A water base paint according to any of claims 37 to 40 and substantially as described in Example 17.
Claim 43
Dependent Claim
59 words
A process for forming a water base paint according to any of claims 37 to 41 comprising a latex rubber-like water base paint binder, a pigment, which process comprises forming a pigment grind of pigment and water together with Biopolymer PS 87 and subsequently incorporating the pigment so treated into the paint binder together with other ingredients as desired.
Claim 44
Dependent Claim
27 words
An aqueous drilling fluid comprising an aqueous medium containing from 0.05 to 3% by weight of Biopolymer PS 87 according to any of claims 1 to 8.
Claim 45
Dependent Claim
14 words
A drilling fluid according to claim 43, in which the aqueous medium comprises brine.
Claim 46
Dependent Claim
13 words
A drilling fluid according to claim 43 or 44, which further comprises oil.
Claim 47
Dependent Claim
16 words
A drilling fluid according to claim 43,44, or 45 and substantially as described in Example 18.
Claim 48
Independent Claim
45 words
A method for drilling a well, which comprises injecting the drilling fluid according to any of claims 43 to 46 into a bore hold and circulating the fluid while drilling - within the bore hole in contact with a drill bit and the underground formation.
Claim 49
Dependent Claim
43 words
A mobility control solution for use in oil recovery comprising an aqueous solution of Biopolymer PS 87 according to any of the claims 1 to 8, in which the Biopolymer PS 87 forms from 0.005 to 0.5% by weight of the control solution.
Claim 50
Dependent Claim
13 words
A mobility control solution according to claim 48, which additionally comprises a salt.
Claim 51
Dependent Claim
30 words
A process for the recovery of crude oil from an oil bearing subterraneous formation, which comprises injecting into the formation a mobility control solution according to claim 48 or 49.
Claim 52
Dependent Claim
72 words
A process for the aqueous suspension polymerisation of a liquid monomer to a particulate polymer in which the monomer is a polymerisable derivative of acrylic acid, or methacrylic acid or its polymerisable derivatives, esters or ethers of vinyl alcohol, vinylidene chloride, styrene or methylstyrene, the process comprising conducting the polymerisation in the presence of from 0.1 to 5% by weight of Biopolymer PS 87 according to any of claims 1 to 8.
Claim 53
Dependent Claim
25 words
A.process according to claim 51, in which Biopolymer PS 87 is present at a concentration of from 1 to 4% by weight of the monomer.
Claim 54
Dependent Claim
29 words
An aqueous, built liquid detergent composition comprising an active detergent material, a builder and, as a stabilising agent, Biopolymer PS 87 according to any of claims 1 to 8.
Claim 55
Dependent Claim
26 words
A composition according to claim 53, in which Biopolymer PS 87 is present in an amount of from 0.05 to 1% by weight of the composition.
Claim 56
Dependent Claim
15 words
A composition according to claim 53 or 54 and substantially as described in Example 19.
References Cited (Backward Citations)
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