Patent US3010111A successfully refreshed from Google Patents.
Patent EP0020251A2 successfully refreshed from Google Patents.
EP0020251A2
Expired / Lapsed
EP
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DNA transfer vector, host transformed with it, vaccine, and their production
Assignee: University of California
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Filing: 1980-05-23
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Granted: —
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Expires: 2000-05-23
Abstract
Abstract DNA transfer vector comprising the genome of a non- passageable virus. The vector can be a plasmid and the virus hepatitis B virus. The production of antigenic protein by the expression of the recombinant DNA in a host cell. The enhancing of the antigenicity and the stability of the antigenic protein by chemical modification.
Inventors
- 👤 William J. Rutter
- 👤 Howard Michael Goodman
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-04 13:41
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/005; C12; C12N; C12N1/00; C12N1/20; C12N1/205; A; A61; A61K; A61K39/00; C12N2730/00; C12N2730/00011; C12N2730/10011; C12N2730/10111; C12N2730/10122; C12R; C12R2001/00; C12R2001/01; C12R2001/185; C12R2001/19
Claims Analysis
Total Claims: 38
Claim 1
Independent Claim
15 words
A DNA transfer vector comprising at least a portion of the genome of an NP-virus.
Claim 2
Dependent Claim
15 words
A DNA transfer vector comprising at least a portion of the genome of an NP-virus.
Claim 3
Dependent Claim
39 words
A DNA transfer vector as in claim 1 wherein said transfer vector is adapted to transform a host cell wherein the transfer vector is replicated and at least a part of the cloned genome portion is expressed by transcription.
Claim 4
Dependent Claim
20 words
The transfer vector of claim 2 wherein at least a part of the cloned genome portion is expressed by translation.
Claim 5
Dependent Claim
13 words
The transfer vector of claim 1 wherein the NP-virus is Hepatitis B Virus.
Claim 6
Dependent Claim
18 words
The transfer vector of claim 2 wherein the vector is a plasmid and the host is a bacterium.
Claim 7
Dependent Claim
19 words
The transfer vector of claim 4 wherein the transfer vector is a plasmid and the host is Escherichia coli.
Claim 8
Dependent Claim
18 words
The transfer vector of claim 6 wherein the vector is pEco-63 and the host is E. coli HB-101.
Claim 9
Independent Claim
104 words
A method for maintaining, replicating, and expressing at least a portion of the genome of an NP-virus comprising, isolating the genetic material comprising at least a portion of the genome of the NP-virus, or a cDNA transcript thereof, recombining said genetic material with a DNA transfer vector, forming a recombinant transfer vector, transforming a host cell with the recombinant transfer vector, selecting a host cell strain capable of maintaining, replicating, and expressing the recombinant transfer vector, and growing the selected host cell under conditions favoring its proliferation, thereby maintaining, replicating, and expressing at least a portion of the genome of the NP-virus carried therein.
Claim 10
Independent Claim
104 words
A method for maintaining, replicating, and expressing at least a portion of the genome of an NP-virus comprising, isolating the genetic material comprising at least a portion of the genome of the NP-virus, or a cDNA transcript thereof, recombining said genetic material with a DNA transfer vector, forming a recombinant transfer vector, transforming a host cell with the recombinant transfer vector, selecting a host cell strain capable of maintaining, replicating, and expressing the recombinant transfer vector, and growing the selected host cell under conditions favoring its proliferation, thereby maintaining, replicating, and expressing at least a portion of the genome of the NP-virus carried therein.
Claim 11
Dependent Claim
12 words
The method of claim 8 wherein the NP-virus is Hepatitis B Virus.
Claim 12
Dependent Claim
18 words
The method of claim 9 wherein the transfer vector is a plasmid and the host is Escherichia coli.
Claim 13
Dependent Claim
54 words
ll. A vaccine against an NP-virus comprising a sterile, physiologically acceptable diluent and an antigen comprising an immunologically active protein constituent of the virus, expressed by a microorganism that has been transformed by a DNA transfer vector comprising a nucleotide sequence encoding said protein, said transformed microorganism being capable of expressing said nucleotide sequence.
Claim 14
Dependent Claim
54 words
ll. A vaccine against an NP-virus comprising a sterile, physiologically acceptable diluent and an antigen comprising an immunologically active protein constituent of the virus, expressed by a microorganism that has been transformed by a DNA transfer vector comprising a nucleotide sequence encoding said protein, said transformed microorganism being capable of expressing said nucleotide sequence.
Claim 15
Dependent Claim
13 words
A vaccine according to claim 11 wherein the NP-virus is Hepatitis B virus.
Claim 16
Dependent Claim
23 words
A vaccine according to claim 11 wherein the protein comprises an immunologically active protein constituent of the surface antigen of Hepatitis B Virus.
Claim 17
Dependent Claim
12 words
A vaccine according to claim 13 wherein the protein comprises the S-protein.
Claim 18
Dependent Claim
17 words
A vaccine according to claim 14 wherein the S-protein comprises additionally the N-terminal amino acid sequence; Met-Gln-Thr-Gln-Lys-Pro-Thr-Pro-Ser-Leu-Ala-Arg-Thr-Gly-Asp-Pro-Val-Thr-Asn-.
Claim 19
Independent Claim
126 words
A method of making a vaccine against an NP-virus comprising the steps of a. transforming a microorganism with a DNA transfer vector comprising a nucleotide sequence encoding a protein of the virus, said nucleotide sequence being inserted in a region of the transfer vector controlled by an expressable operon, in reading frame phase and orientation such that ranslation expression of said operon results in translation expression of said nucleotide sequence, b. growing said microorganism under growth conditions that allow expression of said operon, thereby making a protein comprising the amino acid sequence of said protein of the virus, c. purifying the protein made in step b, and d. mixing the purified protein with a sterile, physiologically acceptable diluent, thereby making a vaccine against an NP virus.
Claim 20
Independent Claim
126 words
A method of making a vaccine against an NP-virus comprising the steps of a. transforming a microorganism with a DNA transfer vector comprising a nucleotide sequence encoding a protein of the virus, said nucleotide sequence being inserted in a region of the transfer vector controlled by an expressable operon, in reading frame phase and orientation such that ranslation expression of said operon results in translation expression of said nucleotide sequence, b. growing said microorganism under growth conditions that allow expression of said operon, thereby making a protein comprising the amino acid sequence of said protein of the virus, c. purifying the protein made in step b, and d. mixing the purified protein with a sterile, physiologically acceptable diluent, thereby making a vaccine against an NP virus.
Claim 21
Dependent Claim
13 words
The method of claim 16 wherein the NP virus is Hepatitis B virus.
Claim 22
Dependent Claim
25 words
The method of claim 16 wherein the protein of the virus comprises an immunologically active protein constituent of the surface antigen of Hepatitis B virus.
Claim 23
Dependent Claim
11 words
The method of claim 18 wherein the protein comprises the S-protein.
Claim 24
Dependent Claim
19 words
The method of claim 19 wherein the protein comprising the S-protein comprises additionally the N-terminal amino acid sequence: Met-Gln-Thr-Gln-Lys-Pro-Thr-Pro-Ser-Leu-Ala-Arg-Thr-Gly-Asp-Pro-Val-Thr-Asn-.
Claim 25
Dependent Claim
30 words
An antigenic protein coded by the genome of an NP virus, synthesized by a microorganism and capable of eliciting antibodies cross-reactive with an immunologically reactive component of said NP virus.
Claim 26
Dependent Claim
30 words
An antigenic protein coded by the genome of an NP virus, synthesized by a microorganism and capable of eliciting antibodies cross-reactive with an immunologically reactive component of said NP virus.
Claim 27
Dependent Claim
13 words
The protein of claim 21, coded by the genome of Hepatitis B virus.
Claim 28
Dependent Claim
17 words
The protein of claim 22 comprising the amino acid sequence of the S-protein of Hepatitis B virus.
Claim 29
Dependent Claim
18 words
A microorganism containing and replicating a DNA transfer vector comprising at least a portion of the HBV genome.
Claim 30
Dependent Claim
18 words
A microorganism containing and replicating a DNA transfer vector comprising at least a portion of the HBV genome.
Claim 31
Dependent Claim
13 words
A microorganism according to claim 24 comprising the bacterial strain Escherichia coli HB101pEco63.
Claim 32
Dependent Claim
7 words
A protein comprising the amino acid sequence:
Claim 33
Dependent Claim
7 words
A protein comprising the amino acid sequence:
Claim 34
Dependent Claim
106 words
A protein having the formula wherein S is the amino acid sequence of the S protein X is H or an amino protecting group or an amino acid, or a peptide comprising at least the C-terminal portion of the amino acid sequence : or a peptide of less than about 4 amino acids length comprising, in random sequence, amino acids selected from the group tyrosine, phenylalanine and tryptophan, and Y is OH, or a carboxyl protecting group, or an amino acid or a peptide of less than about 4 amino acids length comprising, in random sequence, amino acids selected from the group tyrosine, phenylalanine and tryptophan.
Claim 35
Dependent Claim
106 words
A protein having the formula wherein S is the amino acid sequence of the S protein X is H or an amino protecting group or an amino acid, or a peptide comprising at least the C-terminal portion of the amino acid sequence : or a peptide of less than about 4 amino acids length comprising, in random sequence, amino acids selected from the group tyrosine, phenylalanine and tryptophan, and Y is OH, or a carboxyl protecting group, or an amino acid or a peptide of less than about 4 amino acids length comprising, in random sequence, amino acids selected from the group tyrosine, phenylalanine and tryptophan.
Claim 36
Dependent Claim
14 words
A composition containing a protein according to claim 27 in a physiologically acceptable medium.
Claim 37
Dependent Claim
12 words
A protein according to claim 27 having a molecular weight-greater than 25,398.
Claim 38
Dependent Claim
28 words
A protein according to claim 27, wherein at least one asparagine residue in the amino acid subsequence: -Asn-M-(Ser) or (Thr)- is glycosylated, wherein M is any amino acid.
References Cited (Backward Citations)
No citation records indexed.
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