Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
178 words
A process for producing a peptide having the formula X-A-B-C-Y (III) wherein A represents Ala., Gln., Asn., .omega.-BOC-Lys., Leu., Gly., Glu., or Pro; A may be A1 - A2 and A1 represents a hydrophilic amino acid residue and A2 represents Val., Met., Leu. or Gln.; B represents Phe., Tyr., Leu., Met., Glu., Asp., Gln., Asn or Trp,; C represents Phe., Leu., Ileu., Tyr., Cys-SBzl., Ser-OBzl., Trp. or Met.; X represents an .alpha.-amino acid protective group; an amino acid residue having N-terminal protective group or a peptide residue having N-terminal protective group; Y represents a carboxyl protective group; an amino acid residue having C-terminal pro-tective group or a peptide residue having C-terminal protective group, which process comprises reacting a peptide having the formula X-A-B-OH (I) with an amino acid or a peptide having the formula H-C-Y (II) in a weight ratio of the compound of formula (II) to the compound of formula (I) from 1 : 5 to 5 : 1 in the presence of enzyme pepsin in an amount from 0.4 to 400 mg. per mole of reactants.
Claim 2
Dependent Claim
178 words
A process for producing a peptide having the formula X-A-B-C-Y (III) wherein A represents Ala., Gln., Asn., .omega.-BOC-Lys., Leu., Gly., Glu., or Pro; A may be A1 - A2 and A1 represents a hydrophilic amino acid residue and A2 represents Val., Met., Leu. or Gln.; B represents Phe., Tyr., Leu., Met., Glu., Asp., Gln., Asn or Trp,; C represents Phe., Leu., Ileu., Tyr., Cys-SBzl., Ser-OBzl., Trp. or Met.; X represents an .alpha.-amino acid protective group; an amino acid residue having N-terminal protective group or a peptide residue having N-terminal protective group; Y represents a carboxyl protective group; an amino acid residue having C-terminal pro-tective group or a peptide residue having C-terminal protective group, which process comprises reacting a peptide having the formula X-A-B-OH (I) with an amino acid or a peptide having the formula H-C-Y (II) in a weight ratio of the compound of formula (II) to the compound of formula (I) from 1 : 5 to 5 : 1 in the presence of enzyme pepsin in an amount from 0.4 to 400 mg. per mole of reactants.
Claim 3
Dependent Claim
40 words
The process according to Claim 1 in which the peptide of formula I has the formula X-A-B-OH wherein A represents Ala., Gln., Asn., .omega.-BOC-Lys., or Gly.; and B represents Phe., Tyr., Leu., or Met.; X is defined in Claim 1.
Claim 4
Dependent Claim
32 words
The process according to Claim 1 in which the peptide of formula I has the formula X-A-B-OH wherein A represents Glu., , Pro.; and B and X are defined in Claim 1.
Claim 5
Dependent Claim
40 words
The process according to Claim 1 in which the peptide of formula I has the formula wherein A1 represents a hydrophilic amino acid residue and A2 represents Val., Met., Leu. or Gln.; B and X are defined in Claim 1.
Claim 6
Dependent Claim
35 words
The process according to Claim 1 in which in the amino acid or a peptide having the formula H-C-Y C represents Phe., Leu., Ileu., Tyr., Cys-SBzl., or Ser-OBzl; and Y is defined in Claim 1.
Claim 7
Dependent Claim
28 words
The process according to Claim 1 wherein the reaction is effected at a temperature from 20 - 50°C in a buffer solution having pH of 2 - 6.
Claim 8
Dependent Claim
27 words
A process according to Claim 6 in which the temperature is from 30°C to 40°C and the pH of the buffer solution is from 3 - 5.
Claim 9
Dependent Claim
23 words
A process as claimed in Claim 6, 7 or 8 in which the reactant is effected in water or a water miscible medium.
Claim 10
Dependent Claim
34 words
A process as claimed in Claim 6, 7 or 8 in which the ratio of the compound of formula (II) to the compound of formula (I) is 1 : 2 to 2 : 1.