Patent CA1040082B successfully refreshed from Google Patents.
CA1040082B
Expired / Lapsed
CA
⏱️ 0.0 years remaining
Process for the demonstration and determination of reaction components having specific binding affinity for each other
Assignee: Akzona Inc
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Filing: 1978-05-11
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Granted: 1978-10-10
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Expires: 1998-05-11
Abstract
Abstract ABSTRACT OF THE DISCLOSURE The disclosure describes a process for the demonstra-tion and determination of a component of the reaction between a bindable substance which is an antigen, a hapten, or a low molecular substance, and a protein capable of binding the bindable substance specifically. The protein can be an antibody or a specific binding protein. The process involves the steps providing a given quantity of binding partner of the component to be determined in a soluble or insoluble form, reacting the component to be determined with its binding partner, and when the binding partner is in soluble form, adding a given quantity of an antibody against the binding partner, the antibody being in insoluble form. The solid phase is separated from the liquid phase and to the solid phase there is added a coupling product of the component to be determined with an enzyme. The coupling product may also be obtained by binding to an enzyme a substance capable of reacting specifically with the component to be determined or its binding partner. The coupling product is used in quantity at least sufficient to react with all of the binding partner of the component to be determined. Finally, the enzyme activity of the liquid or solid phase of the reaction mixture obtained is determined. Test packs for the performance of this process are also described. This enables to demonstrate or determine substances occurring in extremely low concentra-tions in, for example, serum or urine.
Inventors
- 👤 Antonius H.W.M. Schuurs
- 👤 Bauke K. Van Weemen
Bibliographic Details
Application Number
—
Jurisdiction
CA
Data Source
google_patents
Last Synchronized
2026-11-01 21:46
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54306; G01N33/536; G01N33/537; G01N33/539; G01N33/541; G01N33/74; G01N33/743; Y; Y10; Y10S; Y10S435/00; Y10S435/971; Y10S436/00; Y10S436/807; Y10S436/808; Y10S436/815; Y10S436/817; Y10S436/818
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
187 words
Process for the demonstration and determination of a component of the reaction between a bindable substance selected from the group consisting of an antigen, a hapten, and a low molecular substance, and a protein capable of binding said bind-able substance specifically, said protein being selected from the group consisting of an antibody and a specific binding protein, comprising the steps of: a) providing a given quantity of the binding partner of the component to be determined, which binding partner is in an insoluble form; b) reacting the component to be determined with its binding partner to form a reaction mixture having a solid phase and a liquid phase, c) separating the solid phase from the liquid phase; d) adding to said solid phase a given quantity of a coupling pro-duct of (1) the component to be determined with (2) an enzyme, which quantity of coupling product is at least sufficient to react with all of the binding partner of the component to be determined; and e) determining the enzyme activity of the solid phase which is a measure of the quantity of reaction component to be determined.
Claim 2
Dependent Claim
187 words
Process for the demonstration and determination of a component of the reaction between a bindable substance selected from the group consisting of an antigen, a hapten, and a low molecular substance, and a protein capable of binding said bind-able substance specifically, said protein being selected from the group consisting of an antibody and a specific binding protein, comprising the steps of: a) providing a given quantity of the binding partner of the component to be determined, which binding partner is in an insoluble form; b) reacting the component to be determined with its binding partner to form a reaction mixture having a solid phase and a liquid phase, c) separating the solid phase from the liquid phase; d) adding to said solid phase a given quantity of a coupling pro-duct of (1) the component to be determined with (2) an enzyme, which quantity of coupling product is at least sufficient to react with all of the binding partner of the component to be determined; and e) determining the enzyme activity of the solid phase which is a measure of the quantity of reaction component to be determined.
Claim 3
Dependent Claim
211 words
Process for the demonstration and determination of a component of the reaction between a bindable substance selected from the group consisting of an antigen, a hapten and a low molecular substance, and a protein capable of binding said bind-able substance specifically, said protein being selected from the group consisting of an antibody and a specific binding protein, comprising the steps of: a) providing a given quantity of the binding partner of the component to be determined in a soluble form; b) reacting the component to be determined with its binding partner; c) adding a given quantity of an antibody against said bind-ing partner, which antibody is in an insoluble form, to form a reaction mixture having a solid phase and a liquid phase; d) separating the solid phase from the liquid phase; e) adding to said solid phase a given quantity of a coupling pro-duct of (1) the component to be determined with (2) an enzyme, which quantity of coupling product is at least immuno-chemically equivalent to the quantity, provided in step c), of the insolubilised antibody against said binding partner; and f) determining the enzyme activity of either the liquid phase or the solid phase, which activity is a measure of the quantity of the reaction component to be determined.
Claim 4
Dependent Claim
211 words
Process for the demonstration and determination of a component of the reaction between a bindable substance selected from the group consisting of an antigen, a hapten and a low molecular substance, and a protein capable of binding said bind-able substance specifically, said protein being selected from the group consisting of an antibody and a specific binding protein, comprising the steps of: a) providing a given quantity of the binding partner of the component to be determined in a soluble form; b) reacting the component to be determined with its binding partner; c) adding a given quantity of an antibody against said bind-ing partner, which antibody is in an insoluble form, to form a reaction mixture having a solid phase and a liquid phase; d) separating the solid phase from the liquid phase; e) adding to said solid phase a given quantity of a coupling pro-duct of (1) the component to be determined with (2) an enzyme, which quantity of coupling product is at least immuno-chemically equivalent to the quantity, provided in step c), of the insolubilised antibody against said binding partner; and f) determining the enzyme activity of either the liquid phase or the solid phase, which activity is a measure of the quantity of the reaction component to be determined.
Claim 5
Dependent Claim
12 words
The process of claim 1 in which the enzyme is an oxido-reductase.
Claim 6
Dependent Claim
12 words
The process of claim 2 in which the enzyme is an oxido-reductase.
Claim 7
Dependent Claim
201 words
Process for the demonstration and determination of a component of the reaction between a bindable substance selected from the group consisting of an antigen, a hapten, and a low molecular substance, and a protein capable of binding said bindable substance specifically, said protein being selected from the group consisting of an antibody and a specific binding protein, comprising the steps of: a) providing a given quantity of the binding partner of the component to be determined, which binding partner is in an insoluble form; b) reacting the component to be determined with its binding partner to form a reaction mixture having a solid phase and a liquid phase, c) separating the solid phase from the liquid phase, d) adding to said solid phase a given quantity of a coupling product obtained by binding to an enzyme a substance capable of reacting specifically with one of the reactants of step b), which quantity of coupling product is at least immunochemically equivalent to the quantity, provided in step a), of the insolubilised binding partner of the component to be determined, and e) determining the enzyme activity of the solid phase which is a measure of the quantity of reaction component to be determined.
Claim 8
Dependent Claim
201 words
Process for the demonstration and determination of a component of the reaction between a bindable substance selected from the group consisting of an antigen, a hapten, and a low molecular substance, and a protein capable of binding said bindable substance specifically, said protein being selected from the group consisting of an antibody and a specific binding protein, comprising the steps of: a) providing a given quantity of the binding partner of the component to be determined, which binding partner is in an insoluble form; b) reacting the component to be determined with its binding partner to form a reaction mixture having a solid phase and a liquid phase, c) separating the solid phase from the liquid phase, d) adding to said solid phase a given quantity of a coupling product obtained by binding to an enzyme a substance capable of reacting specifically with one of the reactants of step b), which quantity of coupling product is at least immunochemically equivalent to the quantity, provided in step a), of the insolubilised binding partner of the component to be determined, and e) determining the enzyme activity of the solid phase which is a measure of the quantity of reaction component to be determined.
Claim 9
Dependent Claim
25 words
The process of claim 5 wherein the coupling product is obtained by binding to an enzyme the binding partner of the component to be determined.
Claim 10
Dependent Claim
92 words
A test pack for the performance of the process of claim 6, comprising: a) a given quantity of an insoluble form of the binding partner of the component to be determined, b) a given quantity of a coupling product obtained by binding to an enzyme the binding partner of the component to be determined, which quantity of coupling product is at least immunochemically equivalent to the quantity of the insolub-ilised binding partner of the component to be determined, and c) a substrate for the determination of enzyme activity of said coupling product.
Claim 11
Dependent Claim
36 words
The process of claim 5 wherein the quantity, provided in step a), of the binding partner of the component to be deter-mined is at least sufficient to react with all of the component to be determined.
Claim 12
Dependent Claim
109 words
A test pack for the performance of the process of claim 8, comprising: a) an insoluble form of the binding partner of the component to be determined, in a quantity at least sufficient to react with the maximum amount to be measured of the com-ponent to be determined; b) a given quantity of a coupling product obtained by binding to an enzyme the binding partner of the component to be determined, which quantity of coupling product is at least immunochemically equivalent to the quantity of the in-solubilised binding partner of the component to be determined; and e) a substrate for the determination of enzyme activity of said coupling product.
Claim 13
Dependent Claim
219 words
Process for the demonstration and determination of a component of the reaction between a bindable substance selected from the group consisting of an antigen, a hapten, and a low molecular substance, and a protein capable of binding said bindable substance specifically, said protein being selected from the group consisting of an antibody and a specific binding protein, comprising the steps of: a) providing a given quantity of the binding partner of the component to be determined in a soluble form; b) reacting the component to be determined with its binding partner; e) adding a given quantity of antibody against said binding partner, which antibody is in an insoluble form, to form a reaction mixture having a solid phase and a liquid phase; d) separating the solid phase from the liquid phase; e) adding to said solid phase a given quantity of a coupling product obtained by binding to an enzyme a substance capable of reacting specifically with one of the reactants of step b), which quantity of coupling product is at least immunochemically equivalent to the quantity, provided in step c), of the insolubilised antibody against said binding partner; and f) determining the enzyme activity of either the liquid phase or the solid phase, which activity is a measure of the quantity of the reaction component to be deter-mined.
Claim 14
Dependent Claim
219 words
Process for the demonstration and determination of a component of the reaction between a bindable substance selected from the group consisting of an antigen, a hapten, and a low molecular substance, and a protein capable of binding said bindable substance specifically, said protein being selected from the group consisting of an antibody and a specific binding protein, comprising the steps of: a) providing a given quantity of the binding partner of the component to be determined in a soluble form; b) reacting the component to be determined with its binding partner; e) adding a given quantity of antibody against said binding partner, which antibody is in an insoluble form, to form a reaction mixture having a solid phase and a liquid phase; d) separating the solid phase from the liquid phase; e) adding to said solid phase a given quantity of a coupling product obtained by binding to an enzyme a substance capable of reacting specifically with one of the reactants of step b), which quantity of coupling product is at least immunochemically equivalent to the quantity, provided in step c), of the insolubilised antibody against said binding partner; and f) determining the enzyme activity of either the liquid phase or the solid phase, which activity is a measure of the quantity of the reaction component to be deter-mined.
Claim 15
Dependent Claim
25 words
The process of claim 10 wherein the coupling product is obtained by binding to an enzyme the binding partner of the component to be determined.
Claim 16
Dependent Claim
104 words
A test pack for the performance of the process of claim 11, comprising: a) a given quantity of a soluble form of the binding partner of the component to be determined, b) a given quantity of an insoluble form of an antibody against said binding partner, c) a given quantity of a coupling product obtained by binding to an enzyme the binding partner of the component to be determined, which quantity of coupling product is at least immunochemically equivalent to the quantity of the in-solubilised antibody against said binding partner, and d) a substrate for the determination of enzyme activity of said coupling product.
Claim 17
Dependent Claim
64 words
The process of claim 10 wherein the quantity, provided in step a), of the binding partner of the component to be deter-mined is at least sufficient to react with all of the component to be determined, and wherein the quantity, provided in step c), of the insolubilised antibody against said binding partner is at least sufficient to react with all of said binding partner.
Claim 18
Dependent Claim
131 words
A test pack for the performance of the process of claim 13, comprising: a) a soluble form of the binding partner of the component to be determined, in a quantity at least sufficient to react with the maximum amount to be measured of the component to be determined; b) an insoluble form of an antibody against said binding partner, in a quantity at least sufficient to react with all of said binding partner; c) a given quantity of a coupling product obtained by binding to an enzyme the binding partner of the component to be determined, which quantity of coupling product is at least immunochemically equivalent to the quantity of the in-solubilised antibody against said binding partner; and d) a substrate for the determination of enzyme activity of said coupling product.
References Cited (Backward Citations)
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