USRE28903E
Expired / Lapsed
US
⏱️ 0.0 years remaining
Phosphatide separation
Assignee: Lever Brothers Co
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Filing: 1974-11-25
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Granted: 1976-07-13
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Expires: 1994-11-25
Abstract
Abstract N-acylphosphatides, for instance N-acetylcephalin, are separated from phosphatides without an acylatabe amino group, for instance lecithin, by making a mixture containing them acid to the equivalent of pH less than 3.5 under aqueous conditions and solvent-fractionating with acetone or methyl acetate.
Inventors
- 👤 Rajindra Aneja
- 👤 Jaswinder Singh Chadha
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 12:22
CPC Classifications
A; A23; A23J; A23J7/00; C; C07; C07F; C07F9/00; C07F9/02; C07F9/06; C07F9/08; C07F9/09; C07F9/10; C07F9/103
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
66 words
A process for separating an N-acylcephalin and a phosphatide without an acylatable amino group from a mixture comprising them which comprises adding to the mixture a sufficient amount of an acid to provide the equivalent of pH less than 3.5 under aqueous conditions, extracting the mixture with acetone or methyl acetate, and separating the phases containing the N-acylcephalin and the phosphatide without an acylatable amino group.
Claim 2
Dependent Claim
66 words
A process for separating an N-acylcephalin and a phosphatide without an acylatable amino group from a mixture comprising them which comprises adding to the mixture a sufficient amount of an acid to provide the equivalent of pH less than 3.5 under aqueous conditions, extracting the mixture with acetone or methyl acetate, and separating the phases containing the N-acylcephalin and the phosphatide without an acylatable amino group.
Claim 3
Dependent Claim
75 words
A process for the treatment of phosphatides, which comprises contacting a phosphatide mixture that comprises cephalin .[.as.]. .Iadd.and .Iaddend.a non-N-acylatable phosphatide with an acylating agent to convert the cephalin to N-acylcephalin, adding to .Iadd.the .Iaddend.reaction mixture a sufficient amount of an acid to provide the equivalent of pH less than 3.5 under aqueous conditions, extracting the mixture with acetone or methyl acetate, and separating the phases containing the .[.N-acylphosphatide.]. .Iadd.N-acylcephalin .Iaddend.and the .[.N-acylcephalin.]. .Iadd.non-N-acylatable .Iaddend.phosphatide.
Claim 4
Dependent Claim
75 words
A process for the treatment of phosphatides, which comprises contacting a phosphatide mixture that comprises cephalin .[.as.]. .Iadd.and .Iaddend.a non-N-acylatable phosphatide with an acylating agent to convert the cephalin to N-acylcephalin, adding to .Iadd.the .Iaddend.reaction mixture a sufficient amount of an acid to provide the equivalent of pH less than 3.5 under aqueous conditions, extracting the mixture with acetone or methyl acetate, and separating the phases containing the .[.N-acylphosphatide.]. .Iadd.N-acylcephalin .Iaddend.and the .[.N-acylcephalin.]. .Iadd.non-N-acylatable .Iaddend.phosphatide.
Claim 5
Dependent Claim
14 words
A process according to claim 2, wherein the phosphatide mixture comprises cephalin and lecithin.
Claim 6
Dependent Claim
25 words
A process according to claim 3, wherein the phosphatide mixture contains from 0.1 to 20 parts of lecithin to each part by weight of cephalin.
Claim 7
Dependent Claim
15 words
A process according to claim 3, wherein the acylating agent is a fatty acid anhydride.
Claim 8
Dependent Claim
13 words
A process according to claim 5, wherein the acid anhydride is acetic anhydride.
Claim 9
Dependent Claim
25 words
A process according to claim 5, wherein the completion of acylation is promoted by removal from the reaction mixture of the free fatty acid formed.
Claim 10
Dependent Claim
15 words
A process according to claim 7, wherein free fatty acid formed is removed by distillation.
Claim 11
Dependent Claim
16 words
A process according to claim 8, wherein the acid is removed by evaporation under reduced pressure.
Claim 12
Dependent Claim
12 words
A process according to claim 1, wherein the extraction solvent is acetone.
Claim 13
Dependent Claim
27 words
A process according to claim 1, wherein the reaction mixture containing N-acylcephalin is made acid to the equivalent of pH from 2 to 3 under aqueous conditions.
Claim 14
Dependent Claim
21 words
A process according to claim 11, wherein the acid pH is provided by an acid of pK a less than 2.5.
Claim 15
Dependent Claim
15 words
A process according to claim 1, wherein the acid pH is provided by hydrochloric acid.
Claim 16
Dependent Claim
15 words
A process according to claim 1, wherein the acid pH is provided by orthophosphoric acid.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-07-13 | 1980-01-12 | 1980-07-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-07-13 | 1984-01-12 | 1984-07-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-07-13 | 1988-01-12 | 1988-07-13 | $7700.00 | Window Closed / Lapsed |