Abstract 1. THE METHOD OF PRODUCING A MIXED BACTERIAL CONCENTRATE USEFUL FOR CULTURING MILK WHICH COMPRISES: (A) SEPARATELY INCUBATING IN SEPARATE CULTURE MEDIA TWO TYPES OF BACTERIA, THE FIRST TYPE BEING SELECTED FROM THE GROUP CONSISTING OF STREPTOCOCCUS LACTIS, STREPTOCOCCUS CREMORIS, LACTOBACILLUS BULGARICUS AND STREPTOCOCCUS THERMOPHILUS WHICH PRODUCE LATIC ACID WHEREIN THE INCUBATION CULTURE MEDIUM CONTAINS SKIM MILK AND WHEREIN DURING THE INCUBATION THE LATIC ACID PRODUCED IS NEUTRALIZED AS THE PH OF THE MEDIUM DROPS BY ADDING A NEUTRALIZING AGENT TO MAINTAIN THE GROWTH OF THE BACTERIA, AND THE SECOND TYPE BEING SELECTED FROM THE GROUP CONSISTING OF STREPTOCOCCUS CITROVORUS AND STREPTOCOCCUS PARACITROVORUS, WHEREIN THE CULTURE MEDIUM CONTAINS SKIM MILK OR A DIGEST MILK TO PRODUCE BACTERIA WHICH ARE CENTRIFUGABLE FROM THE MEDIA TO FORM A CONCENTRATE; (B) CONCENTRATING THE RESPECTIVE MEDIA TO OBTAIN SEPARATE CONCENTRATES OF THE TWO BACTERIA (,) CONTAINING AT LEAST ABOUT 10X10**9 CELLS/ML. EACH; (C) MIXING TOGETHER THE TWO TYPES OF BACTERIA IN THE DESIRED PROPORTIONS TO PRODUCE A MIXED CONCENTRATE HAVING CLOSELY CONTROLLED AMOUNTS OF THE TWO TYPES OF BACTERIA THEREI WITHOUT PERMITTING FURTHER GROWTH OF THE BACTERIA; AND (D) THEN FREEZING THE MIXED CONCENTRATE SO THAT IT CAN BE SORED FOR A LONG TIME WITHOUT MAJOR LOSS IN THE VIABILITY OF THE BACTERIA.
THE METHOD OF PRODUCING A MIXED BACTERIAL CONCENTRATE USEFUL FOR CULTURING MILK WHICH COMPRISES: (A) SEPARATELY INCUBATING IN SEPARATE CULTURE MEDIA TWO TYPES OF BACTERIA, THE FIRST TYPE BEING SELECTED FROM THE GROUP CONSISTING OF STREPTOCOCCUS LACTIS, STREPTOCOCCUS CREMORIS, LACTOBACILLUS BULGARICUS AND STREPTOCOCCUS THERMOPHILUS WHICH PRODUCE LATIC ACID WHEREIN THE INCUBATION CULTURE MEDIUM CONTAINS SKIM MILK AND WHEREIN DURING THE INCUBATION THE LATIC ACID PRODUCED IS NEUTRALIZED AS THE PH OF THE MEDIUM DROPS BY ADDING A NEUTRALIZING AGENT TO MAINTAIN THE GROWTH OF THE BACTERIA, AND THE SECOND TYPE BEING SELECTED FROM THE GROUP CONSISTING OF STREPTOCOCCUS CITROVORUS AND STREPTOCOCCUS PARACITROVORUS, WHEREIN THE CULTURE MEDIUM CONTAINS SKIM MILK OR A DIGEST MILK TO PRODUCE BACTERIA WHICH ARE CENTRIFUGABLE FROM THE MEDIA TO FORM A CONCENTRATE; (B) CONCENTRATING THE RESPECTIVE MEDIA TO OBTAIN SEPARATE CONCENTRATES OF THE TWO BACTERIA (,) CONTAINING AT LEAST ABOUT 10X10**9 CELLS/ML. EACH; (C) MIXING TOGETHER THE TWO TYPES OF BACTERIA IN THE DESIRED PROPORTIONS TO PRODUCE A MIXED CONCENTRATE HAVING CLOSELY CONTROLLED AMOUNTS OF THE TWO TYPES OF BACTERIA THEREI WITHOUT PERMITTING FURTHER GROWTH OF THE BACTERIA; AND (D) THEN FREEZING THE MIXED CONCENTRATE SO THAT IT CAN BE SORED FOR A LONG TIME WITHOUT MAJOR LOSS IN THE VIABILITY OF THE BACTERIA.
Claim 2
Independent Claim
208 words
THE METHOD OF PRODUCING A MIXED BACTERIAL CONCENTRATE USEFUL FOR CULTURING MILK WHICH COMPRISES: (A) SEPARATELY INCUBATING IN SEPARATE CULTURE MEDIA TWO TYPES OF BACTERIA, THE FIRST TYPE BEING SELECTED FROM THE GROUP CONSISTING OF STREPTOCOCCUS LACTIS, STREPTOCOCCUS CREMORIS, LACTOBACILLUS BULGARICUS AND STREPTOCOCCUS THERMOPHILUS WHICH PRODUCE LATIC ACID WHEREIN THE INCUBATION CULTURE MEDIUM CONTAINS SKIM MILK AND WHEREIN DURING THE INCUBATION THE LATIC ACID PRODUCED IS NEUTRALIZED AS THE PH OF THE MEDIUM DROPS BY ADDING A NEUTRALIZING AGENT TO MAINTAIN THE GROWTH OF THE BACTERIA, AND THE SECOND TYPE BEING SELECTED FROM THE GROUP CONSISTING OF STREPTOCOCCUS CITROVORUS AND STREPTOCOCCUS PARACITROVORUS, WHEREIN THE CULTURE MEDIUM CONTAINS SKIM MILK OR A DIGEST MILK TO PRODUCE BACTERIA WHICH ARE CENTRIFUGABLE FROM THE MEDIA TO FORM A CONCENTRATE; (B) CONCENTRATING THE RESPECTIVE MEDIA TO OBTAIN SEPARATE CONCENTRATES OF THE TWO BACTERIA (,) CONTAINING AT LEAST ABOUT 10X10**9 CELLS/ML. EACH; (C) MIXING TOGETHER THE TWO TYPES OF BACTERIA IN THE DESIRED PROPORTIONS TO PRODUCE A MIXED CONCENTRATE HAVING CLOSELY CONTROLLED AMOUNTS OF THE TWO TYPES OF BACTERIA THEREI WITHOUT PERMITTING FURTHER GROWTH OF THE BACTERIA; AND (D) THEN FREEZING THE MIXED CONCENTRATE SO THAT IT CAN BE SORED FOR A LONG TIME WITHOUT MAJOR LOSS IN THE VIABILITY OF THE BACTERIA.