Apparatus for density gradient electrophoresis

Abstract

Abstract IN ASSOCIATION WITH ONE OR BOTH ELECTRODES TO PREVENT MIXING OF THE BUFFER SOLUTIONS WITH THE LIQUID IN THE TUBE. APPARATUS FOR SEPARATING AND ISOLATING THE VARIOUS SOLUTES IN COMPLEX MIXTURES BY DENSITY GRADIENT ELECTROPORESIS INCLUDES: A TUBE MADE OF ELECTRICAL INSULATING MATERIAL FOR RECEIVING A DENSITY GRADIANT LIQUID, MEANS INCLUDING A REVERSIBLE PUMP FOR PUMPING A DISPLACING LIQUID INTO AND OUT OF THE TUBE TO POSITION THE DENSITY GRADIANT LIQUID WITHIN A PREDETERMINED PORTION OF THE TUBE, A LIGHT PASSAGEWAY THROUGH THE TUBE, A LIGHT SOURCE POSITIONED TO TRANSMIT LIGHT RAYS THROUGH THE LIGHT PASSAGEWAY, MEANS INCLUDING A LIGHT TRANSDUCER RESPONSIVE TO THE CONCENTRATION OF LIQUID IN THE TUBE AND POSITIONED TO RECEIVE THE LIGHT RAYS TRANSMITTED THROUGH THE LIGHT PASSAGEWAY, A LIGHT FILTER INTERPOSED BETWEEN THE LIGH PASSAGEWAY AND LIGHT TRANSDUCER, AND MEANS ESTABLISHING AN ELECTRIC FIELD ACROSS AXIALLY SPACED POINTS WITHIN THE TUBE. THE MEANS FOR ESTABLISHING THE ELECTRIC FIELD CONSIST OF ELECTRODES CONNECTED TO A SOURCE OF D.C. CURRENT AND IMMERSED IN BUFER SOLUTIONS IN COMMUNICATION WITH THE LIQUID IN THE TUBE; SEMI-PERMEABLE MEMBRANES BEING USED

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