US7318998B2
Expired / Lapsed
US
⏱️ 0.0 years remaining
Tissue decellularization
Assignee: Artivion Inc
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Filing: 2003-03-24
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Granted: 2008-01-15
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Expires: 2023-03-24
Abstract
Abstract The present invention relates, in general, to tissue decellularization and, in particular to a method of treating tissues, for example, heart valves, tendons and ligaments, so as to render them acellular and thereby limit mineralization and/or immunoreactivity upon implementation in vivo.
Inventors
- 👤 Steven Goldstein
- 👤 Kirby S. Black
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 14:06
CPC Classifications
A; A61; A61L; A61L27/00; A61L27/36; A61L27/3683; A61L27/3695; A61L27/3604; A61L27/3687; A61F; A61F2/00; A61F2/02; A61F2/24; A61F2/2412; A61F2/2415; A61L2400/00; A61L2400/02; A61L2430/00; A61L2430/40; Y; Y10; Y10S; Y10S623/00; Y10S623/915; Y10S623/916; Y10S623/918
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
131 words
A method of producing a bioprosthetic tissue consisting essentially of the of the steps of: i) washing a starting tissue selected from the group consisting of a heart valve, tendon, ligament, artery, vein, diaphragm, pericardium, fascia, dura mater and tympanic membrane with a bioburden reducing agent so that said starting tissue is disinfected, ii) subsequent to step (i), contacting said disinfected tissue with a hypotonic solution so that lysis of cells of said disinfected tissue is effected, iii) incubating the hypotonic solution-treated tissue resulting from step (ii) with at least one enzyme, wherein said at least one enzyme consists of at least one nuclease, so that nucleic acid associated with said cells lysed as a result of step (ii) is degraded, and iv) sterilizing the nuclease-treated tissue resulting from step (iii).
Claim 2
Independent Claim
131 words
A method of producing a bioprosthetic tissue consisting essentially of the of the steps of: i) washing a starting tissue selected from the group consisting of a heart valve, tendon, ligament, artery, vein, diaphragm, pericardium, fascia, dura mater and tympanic membrane with a bioburden reducing agent so that said starting tissue is disinfected, ii) subsequent to step (i), contacting said disinfected tissue with a hypotonic solution so that lysis of cells of said disinfected tissue is effected, iii) incubating the hypotonic solution-treated tissue resulting from step (ii) with at least one enzyme, wherein said at least one enzyme consists of at least one nuclease, so that nucleic acid associated with said cells lysed as a result of step (ii) is degraded, and iv) sterilizing the nuclease-treated tissue resulting from step (iii).
Claim 3
Dependent Claim
33 words
The method according to claim 1 wherein said method of production further consists essentially of, after step (iii), the step of contacting the tissue resulting from step (iii) with a physiologically isotonic solution.
Claim 4
Dependent Claim
14 words
The method according to claim 1 wherein said starting tissue is a human tissue.
Claim 5
Dependent Claim
17 words
The method according to claim 3 wherein said starting tissue is obtained from a human heart valve.
Claim 6
Dependent Claim
18 words
The method according to claim 1 wherein said tissue resulting from step (iii) is at least 70% decellularized.
Claim 7
Dependent Claim
17 words
The method according to claim 1 wherein said at least one enzyme consists of DNAase or RNAase.
Claim 8
Dependent Claim
17 words
The method according to claim 1 wherein said at least one enzyme consists of DNAase and RNAase.
Claim 9
Dependent Claim
13 words
The method according to claim 1 wherein said starting tissue is non-human tissue.
Claim 10
Dependent Claim
18 words
The method according to claim 1 wherein said bioburden reducing agent is an antibiotic or mixture of antibiotics.
Claim 11
Dependent Claim
22 words
The method according to claim 1 wherein said step (ii) is carried out at a temperature of between 30° to 40° C.
Claim 12
Dependent Claim
32 words
The method according to claim 1 wherein said method of production further consists essentially of, after step (i), the steps of cryopreserving said disinfected tissue and then thawing said cryopreserved, disinfected tissue.
Claim 13
Dependent Claim
15 words
The method according to claim 1 wherein, in step (ii), said hypotonic solution is water.
References Cited (Backward Citations)
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