US7006710B2
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method and apparatus for correcting crosstalk and spatial resolution for multichannel imaging
Assignee: Cytek Biosciences Inc
•
Filing: 2004-02-20
•
Granted: 2006-02-28
•
Expires: 2024-02-20
Abstract
Abstract A multichannel imaging system generates an ensemble of images for each field of view of an object. Each image in the ensemble is intended to contain information from only one source among a plurality of sources for the object. However, due to crosstalk, at least a portion of the signal from a first source appears in a channel intended for a second source. Because the accuracy of the correction will be degraded if the images in an ensemble are spatially misaligned with respect to one another, the spatial offset between images is determined and a correction is applied to substantially eliminate the offset. Then, a correction to the signals is determined to substantially reduce the contributions to the signal in a channel from the signals in other channels. The signal processing can be employed to process the output signals for each of a plurality of different disclosed imaging systems.
Inventors
- 👤 James K. Riley
- 👤 Keith L. Frost
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 10:18
CPC Classifications
G; G01; G01J; G01J3/00; G01J3/28; G06; G06V; G06V20/00; G06V20/60; G06V20/69; G01J3/2803; G01J3/30; G01J3/32; G01J3/36; G06T; G06T5/00; G06T5/50; G06T7/00; G06T7/30; G06T7/32; G06T7/33; G01N; G01N15/00; G01N15/10; G01N15/14; G01N15/1429; G01N15/1433; G01N15/1468; G01N15/147; G06F; G06F2218/00; G06F2218/02; G06T2207/00; G06T2207/10; G06T2207/10032; G06T2207/10036
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
236 words
A multichannel imaging system for generating an ensemble of images from an object for each field of view of the object, wherein each image in the ensemble contains information from substantially only one source, comprising: (a) a collection lens disposed so that light traveling from the object passes through the collection lens and travels along a collection path; (b) a dispersing component disposed in the collection path so as to receive the light that has passed through the collection lens, dispersing the light into a plurality of light beams, each light beam being directed away from the dispersing component in a different predetermined direction; (c) an imaging lens disposed to receive the light beams from the dispersing component, thereby producing said ensemble of images, each image being produced from a different one of the light beams and being projected by the imaging lens toward a different predetermined location; (d) a multichannel detector disposed to receive the plurality of images produced by the imaging lens, the multichannel detector producing a plurality of output signals, such that a separate output signal is produced for each of said light beams; and (e) means for processing the output signals to: (i) correct the output signals for any misalignment between the images in the ensemble on the multichannel detector to within sub-pixel resolution; and (ii) reduce contributions from the output signals of other channels, to the output signal in each channel.
Claim 2
Dependent Claim
236 words
A multichannel imaging system for generating an ensemble of images from an object for each field of view of the object, wherein each image in the ensemble contains information from substantially only one source, comprising: (a) a collection lens disposed so that light traveling from the object passes through the collection lens and travels along a collection path; (b) a dispersing component disposed in the collection path so as to receive the light that has passed through the collection lens, dispersing the light into a plurality of light beams, each light beam being directed away from the dispersing component in a different predetermined direction; (c) an imaging lens disposed to receive the light beams from the dispersing component, thereby producing said ensemble of images, each image being produced from a different one of the light beams and being projected by the imaging lens toward a different predetermined location; (d) a multichannel detector disposed to receive the plurality of images produced by the imaging lens, the multichannel detector producing a plurality of output signals, such that a separate output signal is produced for each of said light beams; and (e) means for processing the output signals to: (i) correct the output signals for any misalignment between the images in the ensemble on the multichannel detector to within sub-pixel resolution; and (ii) reduce contributions from the output signals of other channels, to the output signal in each channel.
Claim 3
Dependent Claim
239 words
A multichannel imaging system for generating an ensemble of images from an object having a plurality of field of views, for each field of view of the object, wherein each image in the ensemble contains information from substantially only one source, comprising: (a) a collection lens disposed so that light traveling from the object passes through the collection lens and travels along a collection path; (b) a dispersing component disposed in the collection path so as to receive the light that has passed through the collection lens, dispersing the light into a plurality of light beams, each light beam being directed away from the dispersing component in a different predetermined direction; (c) an imaging lens disposed to receive the light beams from the dispersing component, thereby producing said ensemble of images, each image being produced from a different one of the light beams and being projected by the imaging lens toward a different predetermined location; (d) a multichannel detector disposed to receive the plurality of images produced by the imaging lens, the multichannel detector producing a plurality of output signals, such that a separate output signal is produced for each of said light beams; and (e) means for processing the output signals to: (i) correct the output signals for any misalignment between the images in the ensemble on the multichannel detector; and (ii) reduce contributions from the output signals of other channels, to the output signal in each channel.
Claim 4
Dependent Claim
239 words
A multichannel imaging system for generating an ensemble of images from an object having a plurality of field of views, for each field of view of the object, wherein each image in the ensemble contains information from substantially only one source, comprising: (a) a collection lens disposed so that light traveling from the object passes through the collection lens and travels along a collection path; (b) a dispersing component disposed in the collection path so as to receive the light that has passed through the collection lens, dispersing the light into a plurality of light beams, each light beam being directed away from the dispersing component in a different predetermined direction; (c) an imaging lens disposed to receive the light beams from the dispersing component, thereby producing said ensemble of images, each image being produced from a different one of the light beams and being projected by the imaging lens toward a different predetermined location; (d) a multichannel detector disposed to receive the plurality of images produced by the imaging lens, the multichannel detector producing a plurality of output signals, such that a separate output signal is produced for each of said light beams; and (e) means for processing the output signals to: (i) correct the output signals for any misalignment between the images in the ensemble on the multichannel detector; and (ii) reduce contributions from the output signals of other channels, to the output signal in each channel.
Claim 5
Dependent Claim
35 words
The system of claim 2 , further comprising a display electrically coupled to said means for processing, said display producing an image in response to the output signal as processed by said means for processing.
Claim 6
Dependent Claim
89 words
The system of claim 2 , wherein said means for processing comprises: (a) a memory in which a plurality of machine instructions defining a signal conditioning function are stored; and (b) a processor that is coupled to the memory to access the machine instructions, said processor executing said machine instructions and thereby implementing a plurality of functions, including: (i) processing the output signals to spatially align the images detected by the multichannel detector; and (ii) applying a spectral crosstalk correction to the output signals, to remove a channel-to-channel crosstalk.
Claim 7
Dependent Claim
49 words
The system of claim 2 , wherein said multichannel detector comprises a time delay integration (TDI) detector, said TDI detector producing said output signals by integrating light from at least a portion of the object over time, while a relative movement between the object and the imaging system occurs.
Claim 8
Dependent Claim
338 words
A multichannel imaging system for generating an ensemble of images of an object, wherein each image in the ensemble contains information from substantially only a single source from among a plurality of sources, comprising: (a) a first collection lens disposed so that light from the object passes through the first collection lens and travels along a first collection path; (b) a first light dispersing element disposed in the first collection path so as to disperse the light that has passed through the first collection lens, producing a first source from among said plurality of sources; (c) a first imaging lens disposed to receive light from the first source, producing a first image from the first source, said first image being a first one of said ensemble of images; (d) a first detector disposed to receive said first image produced by the first imaging lens, and in response thereto, producing a first output signal; (e) a second collection lens disposed so that light from the object passes through the second collection lens and travels along a second collection path different than the first collection path; (f) a second light dispersing element disposed in the second collection path so as to disperse the light that has passed through the second collection lens, producing a second source from among said plurality of sources; (g) a second imaging lens disposed to receive light from the second source, producing a second image from the second source, said second image comprising a second one of said ensemble of images; (h) a second detector disposed to receive said second image produced by the second imaging lens, producing a second output signal; and (i) means coupled to each detector, for processing the first and the second output signals to perform the following functions: (i) correcting the output signals for misalignment between the images on the first and the second detector; and (ii) substantially reducing contributions to each of the first and the second output signals from the other of the first and the second output signals.
Claim 9
Dependent Claim
338 words
A multichannel imaging system for generating an ensemble of images of an object, wherein each image in the ensemble contains information from substantially only a single source from among a plurality of sources, comprising: (a) a first collection lens disposed so that light from the object passes through the first collection lens and travels along a first collection path; (b) a first light dispersing element disposed in the first collection path so as to disperse the light that has passed through the first collection lens, producing a first source from among said plurality of sources; (c) a first imaging lens disposed to receive light from the first source, producing a first image from the first source, said first image being a first one of said ensemble of images; (d) a first detector disposed to receive said first image produced by the first imaging lens, and in response thereto, producing a first output signal; (e) a second collection lens disposed so that light from the object passes through the second collection lens and travels along a second collection path different than the first collection path; (f) a second light dispersing element disposed in the second collection path so as to disperse the light that has passed through the second collection lens, producing a second source from among said plurality of sources; (g) a second imaging lens disposed to receive light from the second source, producing a second image from the second source, said second image comprising a second one of said ensemble of images; (h) a second detector disposed to receive said second image produced by the second imaging lens, producing a second output signal; and (i) means coupled to each detector, for processing the first and the second output signals to perform the following functions: (i) correcting the output signals for misalignment between the images on the first and the second detector; and (ii) substantially reducing contributions to each of the first and the second output signals from the other of the first and the second output signals.
Claim 10
Dependent Claim
35 words
The system of claim 6 , further comprising a display electrically coupled to said means for processing, said display reproducing an image in response to each output signal as processed by said means for processing.
Claim 11
Dependent Claim
13 words
The system of claim 6 , wherein said means processing comprises an oscilloscope.
Claim 12
Dependent Claim
15 words
The system of claim 6 , wherein said means for processing comprises a programmed computer.
Claim 13
Dependent Claim
17 words
The system of claim 6 , wherein said means for processing comprises an application specific integrated circuit.
Claim 14
Dependent Claim
13 words
The system of claim 6 , wherein each detector comprises a pixilated detector.
Claim 15
Dependent Claim
228 words
A multichannel imaging system for generating an ensemble of images of an object, wherein each image in the ensemble contains information from substantially only a single source from among a plurality of sources, comprising: (a) a collection lens disposed so that light traveling from the object passes through the collection lens and is focussed along a collection path; (b) a dispersing component that receives the light from the collection lens and disperses the light into a plurality of light beams, as a function of a plurality of different discriminable characteristics of the light, each of said plurality of light beams corresponding to a different one of said plurality of sources; (c) at least one pixilated detector; (d) an imaging lens that focuses each of the plurality of light beams on said at least one pixilated detector, producing a respective image corresponding to a different one of the plurality of light beams, each image being one of said ensemble of images, said at least one pixilated detector providing an output signal for each respective image; and (e) a signal processor coupled to receive the output signals from said at least one pixilated detector, said signal processor processing the output signals to: (i) correct the output signals for any misalignment between the respective images on said at least one pixilated detector; and (ii) substantially reducing crosstalk between the output signals.
Claim 16
Dependent Claim
228 words
A multichannel imaging system for generating an ensemble of images of an object, wherein each image in the ensemble contains information from substantially only a single source from among a plurality of sources, comprising: (a) a collection lens disposed so that light traveling from the object passes through the collection lens and is focussed along a collection path; (b) a dispersing component that receives the light from the collection lens and disperses the light into a plurality of light beams, as a function of a plurality of different discriminable characteristics of the light, each of said plurality of light beams corresponding to a different one of said plurality of sources; (c) at least one pixilated detector; (d) an imaging lens that focuses each of the plurality of light beams on said at least one pixilated detector, producing a respective image corresponding to a different one of the plurality of light beams, each image being one of said ensemble of images, said at least one pixilated detector providing an output signal for each respective image; and (e) a signal processor coupled to receive the output signals from said at least one pixilated detector, said signal processor processing the output signals to: (i) correct the output signals for any misalignment between the respective images on said at least one pixilated detector; and (ii) substantially reducing crosstalk between the output signals.
Claim 17
Dependent Claim
49 words
The system of claim 12 , wherein said pixilated detector comprises a time delay integration (TDI) detector, said TDI detector produces said output signals by integrating light from at least a portion of the object over time, while a relative movement between the object and the imaging system occurs.
Claim 18
Dependent Claim
13 words
The system of claim 12 , wherein said signal processor comprises an oscilloscope.
Claim 19
Dependent Claim
14 words
The system of claim 12 , wherein said signal processor comprises a programmed computer.
Claim 20
Dependent Claim
16 words
The system of claim 12 , wherein said signal processor comprises an application specific integrated circuit.
References Cited (Backward Citations)
US3922069A
Cited
US4677680A
Cited
US4635293A
Cited
US4581586A
Cited
US5096807A
Cited
US4770992A
Cited
US4786165A
Cited
US5153916A
Cited
US5159642A
Cited
US5141609A
Cited
US5159397A
Cited
US5159398A
Cited
US5247340A
Cited
US5247339A
Cited
US5548395A
Cited
US5471294A
Cited
US5272354A
Cited
USRE35868E
Cited
US5422712A
Cited
US5444527A
Cited
US5351311A
Cited
US5365525A
Cited
US5674743A
Cited
US5473338A
Cited
US6066459A
Cited
US5596401A
Cited
US5633503A
Cited
US5644388A
Cited
US5613156A
Cited
US5695934A
Cited
US6210973B1
Cited
US5919140A
Cited
US5848123A
Cited
US6007994A
Cited
US5831723A
Cited
US5988862A
Cited
US5959953A
Cited
US5929986A
Cited
US5760899A
Cited
US5754291A
Cited
US5855753A
Cited
US6072765A
Cited
US6014468A
Cited
US6134283A
Cited
US6116739A
Cited
US6553044B1
Cited
US6330081B1
Cited
US6522781B1
Cited
US6549664B1
Cited
WO2000042412A1
Cited
US6256096B1
Cited
US20010006416A1
Cited
US6249341B1
Cited
US6452707B1
Cited
US6381363B1
Cited
US6330361B1
Cited
US6156465A
Cited
US6433904B1
Cited
US6211955B1
Cited
US6662332B1
Cited
US20020126275A1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2009-02-27 | 2009-08-29 | 2010-02-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2013-02-27 | 2013-08-29 | 2014-02-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2017-02-27 | 2017-08-29 | 2018-02-28 | $7700.00 | Window Closed / Lapsed |