US6436255B2
Expired / Lapsed
US
⏱️ 0.0 years remaining
Biosensor
Assignee: Panasonic Holdings Corp
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Filing: 2001-01-19
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Granted: 2002-08-20
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Expires: 2021-01-19
Abstract
Abstract The present invention provides a biosensor which enables prompt quantification by facilitating introduction of a sample solution even when the amount of the sample solution is small and improving dissolution of reagents. The biosensor comprises an insulating base plate; an electrode system including at least a measuring electrode and a counter electrode formed on the base plate; a cover member integrated into the base plate to form a sample solution supply pathway for supplying a sample solution to the electrode system between the cover member and the base plate; a reaction reagent system including at least an electron mediator and an oxidoreductase; and a carrier supporting at least the electron mediator or the oxidoreductase of the reaction reagent system, wherein the carrier is partially or wholly disposed outside the sample solution supply pathway.
Inventors
- 👤 Tomohiro Yamamoto
- 👤 Motokazu Watanabe
- 👤 Shin Ikeda
- 👤 Shiro Nankai
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 23:17
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/005; G; G01; G01N; G01N27/00; G01N27/26; G01N27/28; G01N27/30; G01N27/327; G01N27/3271; G01N27/3272
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
101 words
A biosensor comprising: an insulating base plate; an electrode system including at least a measuring electrode and a counter electrode formed on said base plate; a cover member integrated into said base plate to form a sample solution supply pathway for supplying a sample solution to said electrode system between said cover member and said base plate; a reaction reagent system including at least an electron mediator and an oxidoreductase; and a carrier supporting at least the electron mediator or the oxidoreductase of said reaction reagent system, wherein said carrier is partially or wholly disposed outside said sample solution supply pathway.
Claim 2
Dependent Claim
101 words
A biosensor comprising: an insulating base plate; an electrode system including at least a measuring electrode and a counter electrode formed on said base plate; a cover member integrated into said base plate to form a sample solution supply pathway for supplying a sample solution to said electrode system between said cover member and said base plate; a reaction reagent system including at least an electron mediator and an oxidoreductase; and a carrier supporting at least the electron mediator or the oxidoreductase of said reaction reagent system, wherein said carrier is partially or wholly disposed outside said sample solution supply pathway.
Claim 3
Dependent Claim
39 words
The biosensor in accordance with claim 1 , wherein said carrier has a region supporting at least the electron mediator or the oxidoreductase disposed closer to said electrode system than a region supporting none of said reaction reagent system.
Claim 4
Dependent Claim
14 words
The biosensor in accordance with claim 1 , wherein said oxidoreductase is cholesterol oxidase.
Claim 5
Dependent Claim
21 words
The biosensor in accordance with claim 1 , wherein said reaction reagent system contains an enzyme capable of hydrolyzing cholesterol ester.
Claim 6
Dependent Claim
19 words
The biosensor in accordance with claim 4 , wherein said enzyme capable of hydrolyzing cholesterol ester is cholesterol esterase.
Claim 7
Dependent Claim
16 words
The biosensor in accordance with claim 4 , wherein said reaction reagent system contains a surfactant.
Claim 8
Dependent Claim
13 words
The biosensor in accordance with claim 1 , wherein said carrier is porous.
Claim 9
Dependent Claim
13 words
The biosensor in accordance with claim 7 , wherein said carrier is fibrous.
Claim 10
Dependent Claim
23 words
The biosensor of claim 1 , wherein an end portion of said cover member extends beyond a end portion of said base plate.
Claim 11
Dependent Claim
23 words
The biosensor of claim 1 , wherein an end portion of said base plate extends beyond an end portion of said cover member.
Claim 12
Dependent Claim
114 words
A biosensor comprising: an insulating base plate; an electrode system including at least a measuring electrode and a counter electrode formed on said base plate; a spacer member disposed on said base plate, said spacer member having a slit-like opening; a cover member disposed on said spacer member, said slit-like opening of said spacer member defining a sample solution supply pathway for supplying a sample solution to said electrode system; a reaction reagent system including at least an electron mediator and an oxidoreductase; and a carrier supporting at least the electron mediator or the oxidoreductase of said reaction reagent system, wherein said carrier is partially or wholly disposed outside said sample solution supply pathway.
Claim 13
Dependent Claim
114 words
A biosensor comprising: an insulating base plate; an electrode system including at least a measuring electrode and a counter electrode formed on said base plate; a spacer member disposed on said base plate, said spacer member having a slit-like opening; a cover member disposed on said spacer member, said slit-like opening of said spacer member defining a sample solution supply pathway for supplying a sample solution to said electrode system; a reaction reagent system including at least an electron mediator and an oxidoreductase; and a carrier supporting at least the electron mediator or the oxidoreductase of said reaction reagent system, wherein said carrier is partially or wholly disposed outside said sample solution supply pathway.
Claim 14
Dependent Claim
23 words
The biosensor of claim 11 , wherein an end portion of said cover member extends beyond a end portion of said base plate.
Claim 15
Dependent Claim
23 words
The biosensor of claim 11 , wherein an end portion of said base plate extends beyond an end portion of said cover member.
References Cited (Backward Citations)
JPS61294351A
Cited
JPH0262952A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2005-08-19 | 2006-02-18 | 2006-08-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2009-08-19 | 2010-02-18 | 2010-08-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2013-08-19 | 2014-02-18 | 2014-08-20 | $7700.00 | Window Closed / Lapsed |