US6413783B1
Expired / Lapsed
US
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Assay sonication apparatus and methodology
Assignee: Meso Scale Technologies LLC
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Filing: 1997-09-18
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Granted: 2002-07-02
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Expires: 2017-09-18
Abstract
Abstract An assay apparatus includes a cell with a working electrode and a sonicating device structurally coupled to the cell for sonication the contents of the cell.
Inventors
- 👤 Jacob N. Wohlstadter
- 👤 James Wilbur
- 👤 George Sigal
- 👤 Mark Martin
- 👤 Alan Fischer
- 👤 Larry R. Helms
- 👤 Ramin Darvari
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 03:07
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54373; G01N33/5438; G01N21/00; G01N21/75; G01N21/76; G01N33/5302; Y; Y10; Y10S; Y10S435/00; Y10S435/808; Y10S436/00; Y10S436/805
Claims Analysis
Total Claims: 50
Claim 1
Independent Claim
50 words
An apparatus for use in carrying out a binding assay comprising: (a) a cell comprising one or more electrodes having binding reagents immobilized thereon so as to form one or more binding domains; and (b) a sonication device, structurally coupled to said cell, for sonicating the contents of said cell.
Claim 2
Independent Claim
50 words
An apparatus for use in carrying out a binding assay comprising: (a) a cell comprising one or more electrodes having binding reagents immobilized thereon so as to form one or more binding domains; and (b) a sonication device, structurally coupled to said cell, for sonicating the contents of said cell.
Claim 3
Dependent Claim
49 words
An apparatus as recited in claim 1 , wherein said binding reagents are patterned on said one or more electrodes to form said one or more binding domains and at least one of said binding domains comprises binding reagents differing in specificity from at least one other binding domain.
Claim 4
Dependent Claim
34 words
An apparatus as recited in claim 1 , wherein said one or more electrodes is structurally coupled, through a surface of said cell, to said sonication device for sonicating the contents of said cell.
Claim 5
Dependent Claim
49 words
An apparatus as recited in claim 1 , wherein said binding reagents are patterned on said one or more electrodes to form said one or more binding domains and at least one of said binding domains comprises binding reagents differing in specificity from at least one other binding domain.
Claim 6
Dependent Claim
19 words
An apparatus as recited in claim 1 , wherein said one or more electrodes comprise a carbon-containing polymer composite.
Claim 7
Dependent Claim
17 words
An apparatus as recited in claim 1 , wherein said one or more electrodes comprise fibril-polymer composites.
Claim 8
Dependent Claim
40 words
An apparatus as recited in claim 1 , wherein said one or more electrodes are working electrodes and said cell further comprises one or more counter electrodes that cooperate with said one or more working electrodes to conduct electrochemiluminescenece assays.
Claim 9
Independent Claim
73 words
An apparatus for use in carrying out a binding assay, comprising: (a) a cell comprising one or more solid phase supports, said supports having binding reagents immobilized and patterned thereon into a plurality of distinct binding domains wherein at least one of said binding domains comprises binding reagents differing in specificity from at least one other binding domain; and a sonication device, structurally coupled to said cell, for sonicating contents of said cell.
Claim 10
Independent Claim
73 words
An apparatus for use in carrying out a binding assay, comprising: (a) a cell comprising one or more solid phase supports, said supports having binding reagents immobilized and patterned thereon into a plurality of distinct binding domains wherein at least one of said binding domains comprises binding reagents differing in specificity from at least one other binding domain; and a sonication device, structurally coupled to said cell, for sonicating contents of said cell.
Claim 11
Dependent Claim
36 words
An apparatus as recited in claim 8 , wherein said one or more solid phase supports is structurally coupled, through a surface of said cell, to said sonication device for sonicating the contents of said cell.
Claim 12
Independent Claim
81 words
An apparatus for use in carrying out a binding assay, comprising: (a) a cell comprising one or more solid phase supports, said supports having one or more binding reagents immobilized thereon forming one or more binding domains; and (b) a sonication device, structurally coupled to said cell, for sonicating contents of said cell, wherein said one or more solid phase supports is structurally coupled, through a surface of said cell, to said sonication device for sonicating the contents of said cell.
Claim 13
Independent Claim
81 words
An apparatus for use in carrying out a binding assay, comprising: (a) a cell comprising one or more solid phase supports, said supports having one or more binding reagents immobilized thereon forming one or more binding domains; and (b) a sonication device, structurally coupled to said cell, for sonicating contents of said cell, wherein said one or more solid phase supports is structurally coupled, through a surface of said cell, to said sonication device for sonicating the contents of said cell.
Claim 14
Dependent Claim
48 words
An apparatus as recited in claim 10 , wherein said binding reagents are patterned on said solid phase supports to form said one or more binding domains and at least one of said binding domains comprises binding reagents differing in specificity from at least one other binding domain.
Claim 15
Independent Claim
20 words
An apparatus as recited in claims 1 , 8 or 10 , wherein said sonication device is a piezoelectric device.
Claim 16
Independent Claim
34 words
An apparatus as recited in claims 1 , 8 or 10 , said cell further including thin capillaries, wherein operation of said sonication device increases the rate of fluid flow through said thin capillaries.
Claim 17
Independent Claim
22 words
The apparatus according to claims 1 , 8 or 10 , further comprising a light detector for detecting luminescence from said cell.
Claim 18
Independent Claim
31 words
An apparatus as recited in claims 1 , 8 or 10 , wherein said binding reagents are selected from the group consisting of antibodies, antibody fragments, enzymes, nucleic acids and receptors.
Claim 19
Independent Claim
22 words
An apparatus as recited in claims 1 , 8 or 10 , wherein said apparatus detects specific binding reactions within said cell.
Claim 20
Independent Claim
28 words
An apparatus as recited in claims 1 , 8 or 10 , wherein said sonication device is capable of providing sonication energy at from 0.1 to 10,000 kHz.
Claim 21
Independent Claim
25 words
An apparatus as recited in claims 1 , 8 or 10 , wherein said sonication device has a power of from 0.001 to 10 watts.
Claim 22
Independent Claim
17 words
An apparatus as recited in claims 1 , 8 or 10 , wherein said apparatus is portable.
Claim 23
Dependent Claim
20 words
An apparatus as recited in claim 1 , 8 or 10 , wherein said sonication device is an electromagnetic actuator.
Claim 24
Independent Claim
20 words
An apparatus as recited in claims 1 , 8 or 10 , wherein said cell comprises a plurality of wells.
Claim 25
Independent Claim
19 words
An apparatus as recited in claims 1 , 8 or 10 , wherein said cell comprises a well plate.
Claim 26
Independent Claim
25 words
An apparatus as recited in claims 1 , 8 or 10 , wherein said cell comprises a 96 well plate or a 384 well plate.
Claim 27
Independent Claim
56 words
An apparatus for use in carrying out a binding assay, comprising: (a) a cell; (b) a sonication device, structurally coupled to said cell, for sonicating the contents of said cell; and (c) one or more solid phase supports, said supports having one or more binding domains formed thereon, wherein said sonication device is an electromagnetic actuator.
Claim 28
Independent Claim
56 words
An apparatus for use in carrying out a binding assay, comprising: (a) a cell; (b) a sonication device, structurally coupled to said cell, for sonicating the contents of said cell; and (c) one or more solid phase supports, said supports having one or more binding domains formed thereon, wherein said sonication device is an electromagnetic actuator.
Claim 29
Independent Claim
91 words
An apparatus for use in carrying out a binding assay comprising a cell, said cell comprising: (a) one or more solid phase supports having one or more binding domains formed thereon and wherein said one or more solid phase supports are working electrodes; (b) means, structurally coupled to said one or more solid phase supports or to said cell, for sonicating contents of said cell; and (c) one or more counter electrodes, wherein said one or more working electrodes and said one or more counter electrodes cooperate to conduct electrochemiluminescence assays.
Claim 30
Independent Claim
91 words
An apparatus for use in carrying out a binding assay comprising a cell, said cell comprising: (a) one or more solid phase supports having one or more binding domains formed thereon and wherein said one or more solid phase supports are working electrodes; (b) means, structurally coupled to said one or more solid phase supports or to said cell, for sonicating contents of said cell; and (c) one or more counter electrodes, wherein said one or more working electrodes and said one or more counter electrodes cooperate to conduct electrochemiluminescence assays.
Claim 31
Dependent Claim
30 words
An apparatus as recited in claim 25 , said cell further including thin capillaries, wherein operation of said sonication means increases the rate of fluid flow through said thin capillaries.
Claim 32
Dependent Claim
18 words
An apparatus as recited in claim 25 , wherein said apparatus detects specific binding reactions within said cell.
Claim 33
Dependent Claim
16 words
An apparatus as recited in claim 25 , wherein said sonication means is a piezoelectric device.
Claim 34
Dependent Claim
16 words
An apparatus as recited in claim 25 , wherein said sonication means is an electromagnetic actuator.
Claim 35
Dependent Claim
29 words
An apparatus as recited in claim 25 , wherein said binding domains include binding reagents selected from the group consisting of antibodies, antibody fragments, enzymes, nucleic acids and receptors.
Claim 36
Dependent Claim
24 words
An apparatus as recited in claim 25 , wherein said sonication means is capable of providing sonication energy at from 0.1 to 10,000 kHz.
Claim 37
Dependent Claim
21 words
An apparatus as recited in claim 25 , wherein said sonication means has a power of from 0.001 to 10 watts.
Claim 38
Dependent Claim
22 words
An apparatus as recited in claim 25 , further comprising a light detector for detecting or measuring luminescence emitted within said cell.
Claim 39
Dependent Claim
20 words
An apparatus as recited in claim 25 , wherein said one or more solid-phase supports comprise a carbon-containing polymer composite.
Claim 40
Dependent Claim
18 words
An apparatus as recited in claim 25 , wherein said one or more solid-phase supports comprise fibril-polymer composites.
Claim 41
Independent Claim
66 words
An apparatus for use in carrying out a binding assay comprising a cell, said cell comprising: (a) one or more solid phase supports having one or more binding domains formed thereon; and (b) means, structurally coupled to said one or more solid phase supports or to said cell, for sonicating contents of said cell, wherein said one or more solid-phase supports comprise a carbon-containing polymer composite.
Claim 42
Independent Claim
66 words
An apparatus for use in carrying out a binding assay comprising a cell, said cell comprising: (a) one or more solid phase supports having one or more binding domains formed thereon; and (b) means, structurally coupled to said one or more solid phase supports or to said cell, for sonicating contents of said cell, wherein said one or more solid-phase supports comprise a carbon-containing polymer composite.
Claim 43
Independent Claim
64 words
An apparatus for use in carrying out a binding assay comprising a cell, said cell comprising: (a) one or more solid phase supports having one or more binding domains formed thereon; and (b) means, structurally coupled to said one or more solid phase supports or to said cell, for sonicating contents of said cell, wherein said one or more solid-phase supports comprise fibril-polymer composites.
Claim 44
Independent Claim
64 words
An apparatus for use in carrying out a binding assay comprising a cell, said cell comprising: (a) one or more solid phase supports having one or more binding domains formed thereon; and (b) means, structurally coupled to said one or more solid phase supports or to said cell, for sonicating contents of said cell, wherein said one or more solid-phase supports comprise fibril-polymer composites.
Claim 45
Independent Claim
55 words
An apparatus for use in carrying out a binding assay, comprising: (a) a cell; (b) a sonication device, structurally coupled to said cell, for sonicating contents of said cell; and (c) one or more solid phase supports, said supports having one or more binding domains formed thereon, wherein said cell comprises a plurality of wells.
Claim 46
Independent Claim
55 words
An apparatus for use in carrying out a binding assay, comprising: (a) a cell; (b) a sonication device, structurally coupled to said cell, for sonicating contents of said cell; and (c) one or more solid phase supports, said supports having one or more binding domains formed thereon, wherein said cell comprises a plurality of wells.
Claim 47
Independent Claim
54 words
An apparatus for use in carrying out a binding assay, comprising: (a) a cell; (b) a sonication device, structurally coupled to said cell, for sonicating contents of said cell; and (c) one or more solid phase supports, said supports having one or more binding domains formed thereon, wherein said cell comprises a well plate.
Claim 48
Independent Claim
54 words
An apparatus for use in carrying out a binding assay, comprising: (a) a cell; (b) a sonication device, structurally coupled to said cell, for sonicating contents of said cell; and (c) one or more solid phase supports, said supports having one or more binding domains formed thereon, wherein said cell comprises a well plate.
Claim 49
Independent Claim
60 words
An apparatus for use in carrying out a binding assay, comprising: (a) a cell; (b) a sonication device, structurally coupled to said cell, for sonicating contents of said cell; and (c) one or more solid phase supports, said supports having one or more binding domains formed thereon, wherein said cell comprises a 96 well plate or a 384 well plate.
Claim 50
Independent Claim
60 words
An apparatus for use in carrying out a binding assay, comprising: (a) a cell; (b) a sonication device, structurally coupled to said cell, for sonicating contents of said cell; and (c) one or more solid phase supports, said supports having one or more binding domains formed thereon, wherein said cell comprises a 96 well plate or a 384 well plate.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2005-07-01 | 2005-12-31 | 2006-07-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2009-07-01 | 2009-12-31 | 2010-07-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2013-07-01 | 2013-12-31 | 2014-07-02 | $7700.00 | Window Closed / Lapsed |