US6159232A
Expired / Lapsed
US
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Clotting cascade initiating apparatus and methods of use and methods of closing wounds
Assignee: Closys Corp
•
Filing: 1998-12-15
•
Granted: 2000-12-12
•
Expires: 2018-12-15
Abstract
Abstract Wound closure methods and apparatus are provided which utilize blood fluid by activating the clotting cascade of the blood fluid outside the body within a substantially enclosed sterile container then introducing the blood fluid to the wound site to complete clotting. Methods and apparatus for providing ways of inhibiting anticoagulants and slowing fibrin clot degradation are also disclosed. Kits for practicing the invention singularly or in combination with and/or associated with preferred procedures are also disclosed.
Inventors
- 👤 Karol L. Nowakowski
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 15:05
CPC Classifications
A; A61; A61L; A61L24/00; A61L24/0005; A61B; A61B17/00; A61B17/00491; A61B17/0057; A61B2017/00637; A61B2017/00641; A61B2017/00646; A61B2017/0065; A61M; A61M25/00; A61M25/01; A61M25/06; A61M25/0662; A61M2025/0681
Claims Analysis
Total Claims: 69
Claim 1
Independent Claim
156 words
A method of closing a wound in a patient, the method comprising the steps of: a) treating a blood fluid with a procoagulating, agent to initiate a clotting cascade during a first time period such that at least a portion of the blood fluid will form a clot of blood during a second time period subsequent to the first time period, the blood fluid containing sufficient blood components to enable a portion of the blood fluid to clot during the second time period subsequent to the initiation of the clotting cascade during the first time period; the blood fluid being whole blood; and b) transporting the treated blood fluid to the wound in the patient such that the blood fluid comes into contact with the patient proximate the wound and the clotting cascade comes generally to a conclusion and a clot forms proximate the wound such that the clot prevents fluid from passing through the wound.
Claim 2
Independent Claim
156 words
A method of closing a wound in a patient, the method comprising the steps of: a) treating a blood fluid with a procoagulating, agent to initiate a clotting cascade during a first time period such that at least a portion of the blood fluid will form a clot of blood during a second time period subsequent to the first time period, the blood fluid containing sufficient blood components to enable a portion of the blood fluid to clot during the second time period subsequent to the initiation of the clotting cascade during the first time period; the blood fluid being whole blood; and b) transporting the treated blood fluid to the wound in the patient such that the blood fluid comes into contact with the patient proximate the wound and the clotting cascade comes generally to a conclusion and a clot forms proximate the wound such that the clot prevents fluid from passing through the wound.
Claim 3
Dependent Claim
54 words
The method of claim 1, the step of treating the blood fluid including (1) providing a containment chamber in which the blood fluid can be contained; (2) placing the blood fluid in the containment chamber; and (3) initiating the clotting cascade by exposing the blood fluid to a procoagulating agent within the containment chamber.
Claim 4
Dependent Claim
26 words
The method of claim 2, the step of treating the blood fluid including providing a substantially enclosed sterile containment chamber, the chamber containing a procoagulating agent.
Claim 5
Dependent Claim
30 words
The method of claim 3, wherein the procoagulating agent is a porous matrix and the step of treating the blood fluid includes passing the blood fluid through the porous matrix.
Claim 6
Dependent Claim
41 words
The method of claim 4, wherein the porous matrix is biological in origin and the step of treating the blood fluid includes exposing the blood fluid to the biologic material such that the clotting cascade is initiated within the blood fluid.
Claim 7
Dependent Claim
17 words
The method of claim 5, wherein the biologic material is a biologic agent is cotton, diatomaceous earth.
Claim 8
Dependent Claim
41 words
The method of claim 4, wherein the porous matrix is an inorganic material and the step of treating the blood fluid includes exposing the blood fluid to the inorganic material such that the clotting cascade is initiated within the blood fluid.
Claim 9
Dependent Claim
11 words
The method of claim 7, wherein the inorganic material is glass.
Claim 10
Dependent Claim
41 words
The method of claim 4, wherein the porous matrix is a synthetic polymer and the step of treating the blood fluid includes exposing the blood fluid to the synthetic polymer such that the clotting cascade is initiated within the blood fluid.
Claim 11
Dependent Claim
29 words
The method of claim 9, wherein the synthetic polymer agent is a synthetic polymer selected from the group consisting of dacron, silicone, polyurethane, polycarbonate, polyethylene, polyvinylchloride, polypropylene and nylon.
Claim 12
Dependent Claim
34 words
The method of claim 1, further comprising the step of applying direct pressure to an artery proximal to a wound in the artery thereby reducing arterial blood pressure while clot forms in the wound.
Claim 13
Dependent Claim
25 words
The method of claim 1, further comprising the step of treating a blood fluid including treatment of the blood fluid with an anticoagulant inhibiting agent.
Claim 14
Dependent Claim
24 words
The method of claim 12, the step of treating a blood fluid further including treatment of the blood fluid with an anticlot inhibiting agent.
Claim 15
Dependent Claim
23 words
The method of claim 1, the step of treating a blood fluid including treatment of the blood fluid with an anticlot inhibiting agent.
Claim 16
Dependent Claim
173 words
A clotting cascade initiation apparatus within which a blood fluid can be received and retained, the blood fluid being useful for treatment of a wound within a patient, said apparatus comprising: a substantially enclosed sterile containment chamber within which the blood fluid can be received and retained, the substantially enclosed sterile containment chamber containing a procoagulating agent; and a cannulated delivery member in fluid communication with the containment chamber such that blood fluid can pass out of the containment chamber and through the cannulated delivery member; wherein a clotting cascade is initiated when the blood fluid is received in the sterile containment chamber and exposed to the procoagulating agent within the chamber, and subsequently delivered via the cannulated delivery member to the wound subsequent to the initiation of the clotting cascade, such that the clotting cascade, initiated within the sterile containment chamber prior to the delivery of the blood fluid to the wound, can be completed within the wound and a clot can form within the wound when the clotting cascade is completed.
Claim 17
Dependent Claim
173 words
A clotting cascade initiation apparatus within which a blood fluid can be received and retained, the blood fluid being useful for treatment of a wound within a patient, said apparatus comprising: a substantially enclosed sterile containment chamber within which the blood fluid can be received and retained, the substantially enclosed sterile containment chamber containing a procoagulating agent; and a cannulated delivery member in fluid communication with the containment chamber such that blood fluid can pass out of the containment chamber and through the cannulated delivery member; wherein a clotting cascade is initiated when the blood fluid is received in the sterile containment chamber and exposed to the procoagulating agent within the chamber, and subsequently delivered via the cannulated delivery member to the wound subsequent to the initiation of the clotting cascade, such that the clotting cascade, initiated within the sterile containment chamber prior to the delivery of the blood fluid to the wound, can be completed within the wound and a clot can form within the wound when the clotting cascade is completed.
Claim 18
Dependent Claim
28 words
The clotting cascade initiation apparatus of claim 15, further including a cannula for delivering blood fluid to the wound, the cannula being in communication with the containment chamber.
Claim 19
Dependent Claim
35 words
The clotting cascade initiation apparatus of claim 15, further including a cannula for withdrawing blood fluid from the patient and delivering blood fluid to the wound, the cannula being in communication with the containment chamber.
Claim 20
Dependent Claim
24 words
The clotting cascade initiation apparatus of claim 15, wherein the procoagulating agent is a porous matrix through which the blood fluid can be passed.
Claim 21
Dependent Claim
16 words
The clotting cascade initiation apparatus of claim 18, wherein the porous matrix is an inorganic material.
Claim 22
Dependent Claim
14 words
The clotting cascade initiation apparatus of claim 19, wherein the inorganic material is glass.
Claim 23
Dependent Claim
16 words
The clotting cascade initiation apparatus of claim 18, wherein the porous matrix is a synthetic polymer.
Claim 24
Dependent Claim
31 words
The clotting cascade initiation apparatus of claim 21, wherein the synthetic polymer is a synthetic polymer selected from the group consisting of dacron, silicone, polyurethane, polycarbonate, polyethylene, polyvinylchloride, polypropylene and nylon.
Claim 25
Dependent Claim
16 words
The clotting cascade initiation apparatus of claim 18, wherein the porous matrix is biological in origin.
Claim 26
Dependent Claim
25 words
The clotting cascade initiation apparatus of claim 23, wherein the biologic material is a biologic material selected from the group consisting of cotton, diatomaceous earth.
Claim 27
Independent Claim
185 words
A method of closing a wound in a patient, said method comprising the steps of: a) providing a clotting cascade initiation apparatus within which a blood fluid can be received, said apparatus including a substantially enclosed sterile containment chamber within which the blood fluid can be received and retained, and a cannulated delivery member in fluid communication with the containment chamber; the containment chamber containing a procoagulating agent; wherein a clotting cascade can be initiated when the blood fluid is placed into the sterile containment chamber and thereby exposed to the procoagulating agent; b) placing a sufficient amount of blood fluid into the containment chamber to provide a sufficient amount of blood fluid to enable the blood fluid to knit together to form a clot proximate the wound once the clotting cascade is initiated; and c) delivering the blood fluid to the wound via the cannulated delivery member subsequent to the initiation of the clotting cascade in the containment chamber such that the clotting cascade can be completed and a clot can form within the wound after the blood fluid is delivered to the wound.
Claim 28
Independent Claim
185 words
A method of closing a wound in a patient, said method comprising the steps of: a) providing a clotting cascade initiation apparatus within which a blood fluid can be received, said apparatus including a substantially enclosed sterile containment chamber within which the blood fluid can be received and retained, and a cannulated delivery member in fluid communication with the containment chamber; the containment chamber containing a procoagulating agent; wherein a clotting cascade can be initiated when the blood fluid is placed into the sterile containment chamber and thereby exposed to the procoagulating agent; b) placing a sufficient amount of blood fluid into the containment chamber to provide a sufficient amount of blood fluid to enable the blood fluid to knit together to form a clot proximate the wound once the clotting cascade is initiated; and c) delivering the blood fluid to the wound via the cannulated delivery member subsequent to the initiation of the clotting cascade in the containment chamber such that the clotting cascade can be completed and a clot can form within the wound after the blood fluid is delivered to the wound.
Claim 29
Dependent Claim
40 words
The method of closing a wound in a patient of claim 25, wherein the procoagulating agent is a porous matrix and the step of placing the blood fluid in the chamber includes passing the blood fluid through the porous matrix.
Claim 30
Dependent Claim
20 words
The method of closing a wound in a patient of claim 25, wherein the procoagulating agent is biological in origin.
Claim 31
Dependent Claim
29 words
The method of closing a wound in a patient of claim 27, wherein the biologic material is a biologic material selected from the group consisting of cotton, diatomaceous earth.
Claim 32
Dependent Claim
18 words
The method of closing a wound in a patient of claim 25, wherein the procoagulating agent is inorganic.
Claim 33
Dependent Claim
18 words
The method of closing a wound in a patient of claim 29, wherein the inorganic agent is glass.
Claim 34
Dependent Claim
20 words
The method of closing a wound in a patient of claim 25, wherein the procoagulating agent is a synthetic polymer.
Claim 35
Dependent Claim
35 words
The method of closing a wound in a patient of claim 31, wherein the synthetic polymer is a synthetic polymer selected from the group consisting of dacron, silicone, polyurethane, polycarbonate, polyethylene, polyvinylchloride, polypropylene and nylon.
Claim 36
Dependent Claim
34 words
The method of claim 15, further comprising the step of applying direct pressure to an artery proximal to a wound in the artery thereby reducing arterial blood pressure while clot forms in the wound.
Claim 37
Independent Claim
170 words
A method of closing a wound in a patient, said method comprising the steps of: a) providing a clotting cascade initiation apparatus within which a blood fluid can be received and a clotting cascade can be initiated, said apparatus including a substantially enclosed sterile containment chamber within which the blood fluid can be received and retained; b) placing a sufficient amount of blood fluid into the containment chamber to provide a sufficient amount of blood fluid to enable the blood fluid to knit together to form a clot once the clotting cascade is initiated, the blood fluid being whole blood; c) placing a procoagulating agent within the substantially enclosed sterile containment chamber such that the procoagulating agent comes into contact with the blood fluid and a clotting cascade is initiated in blood fluid; and d) delivering the blood fluid to the wound subsequent to the initiation of the clotting cascade in the containment chamber such that the clotting cascade can be completed and a clot can form within the wound.
Claim 38
Independent Claim
170 words
A method of closing a wound in a patient, said method comprising the steps of: a) providing a clotting cascade initiation apparatus within which a blood fluid can be received and a clotting cascade can be initiated, said apparatus including a substantially enclosed sterile containment chamber within which the blood fluid can be received and retained; b) placing a sufficient amount of blood fluid into the containment chamber to provide a sufficient amount of blood fluid to enable the blood fluid to knit together to form a clot once the clotting cascade is initiated, the blood fluid being whole blood; c) placing a procoagulating agent within the substantially enclosed sterile containment chamber such that the procoagulating agent comes into contact with the blood fluid and a clotting cascade is initiated in blood fluid; and d) delivering the blood fluid to the wound subsequent to the initiation of the clotting cascade in the containment chamber such that the clotting cascade can be completed and a clot can form within the wound.
Claim 39
Dependent Claim
47 words
The method of closing a wound in a patient of claim 34, wherein the step of placing the procoagulating agent within the substantially enclosed sterile containment chamber includes placing a biocompatible procoagulating agent within the chamber such that the procoagulating agent is mixed with the blood fluid.
Claim 40
Dependent Claim
21 words
The method of closing a wound in a patient of claim 35, wherein the biocompatible procoagulating agent is a biological material.
Claim 41
Dependent Claim
32 words
The method of closing a wound in a patient of claim 36, wherein the biological material is a biocompatible procoagulating agent selected from the group consisting of thrombin, collagen, fibrin and fibrinogen.
Claim 42
Dependent Claim
20 words
The method of closing a wound in a patient claim 35, wherein the biocompatible procoagulating agent is a synthetic compound.
Claim 43
Dependent Claim
21 words
The method of closing a wound in a patient of claim 38, wherein the synthetic biocompatible procoagulating agent is aminocaproic acid.
Claim 44
Dependent Claim
21 words
The method of closing a wound in a patient of claim 35, wherein the biocompatible procoagulating agent is an inorganic compound.
Claim 45
Dependent Claim
21 words
The method of closing a wound in a patient of claim 35, wherein the inorganic biocompatible procoagulating agent is a salt.
Claim 46
Independent Claim
160 words
A method of closing a wound in a patient, said method comprising the steps of: a) providing a clotting cascade initiation apparatus within which a blood fluid can be received and a clotting cascade can be initiated, said apparatus including a substantially enclosed sterile containment chamber within which the blood fluid can be received and retained; b) placing a sufficient amount of blood fluid into the containment chamber to provide a sufficient amount of blood fluid to enable the blood fluid to knit together to form a clot once the clotting cascade is initiated, the blood fluid being whole blood; c) mechanically stressing the blood fluid within the substantially enclosed sterile containment chamber such that a clotting cascade is initiated in blood fluid, and d) delivering the blood fluid to the wound subsequent to the initiation of the clotting cascade in the containment chamber such that the clotting cascade can be completed and a clot can form within the wound.
Claim 47
Independent Claim
160 words
A method of closing a wound in a patient, said method comprising the steps of: a) providing a clotting cascade initiation apparatus within which a blood fluid can be received and a clotting cascade can be initiated, said apparatus including a substantially enclosed sterile containment chamber within which the blood fluid can be received and retained; b) placing a sufficient amount of blood fluid into the containment chamber to provide a sufficient amount of blood fluid to enable the blood fluid to knit together to form a clot once the clotting cascade is initiated, the blood fluid being whole blood; c) mechanically stressing the blood fluid within the substantially enclosed sterile containment chamber such that a clotting cascade is initiated in blood fluid, and d) delivering the blood fluid to the wound subsequent to the initiation of the clotting cascade in the containment chamber such that the clotting cascade can be completed and a clot can form within the wound.
Claim 48
Dependent Claim
42 words
The method of closing a wound in a patient of claim 42, wherein the step of mechanically stressing the blood fluid within the substantially enclosed sterile containment chamber is selected from the group consisting of blood fluid agitation, thermal induction and lysis.
Claim 49
Independent Claim
196 words
A method of closing a wound in a patient, said method comprising the steps of: a) providing a clotting cascade initiation apparatus within which a blood fluid can be received, said apparatus including a substantially enclosed sterile containment chamber within which the blood fluid can be received and retained, and a cannulated delivery member in fluid communication with the containment chamber; the containment chamber containing a procoagulating agent; wherein a clotting cascade can be initiated when the blood fluid is placed into the sterile containment chamber and thereby exposed to the procoagulating agent; b) drawing a sufficient amount of an autologous blood fluid from the patient into the containment chamber to provide a sufficient amount of blood fluid to the wound, once the clotting cascade is initiated, to enable the blood fluid to knit together to form a clot, the blood fluid being whole blood; and c) delivering the blood fluid to the wound via the cannulated delivery member subsequent to the initiation of the clotting cascade in the containment chamber so that the clotting cascade can be completed and a clot can fonn within the wound after the blood fluid is delivered to the wound.
Claim 50
Independent Claim
196 words
A method of closing a wound in a patient, said method comprising the steps of: a) providing a clotting cascade initiation apparatus within which a blood fluid can be received, said apparatus including a substantially enclosed sterile containment chamber within which the blood fluid can be received and retained, and a cannulated delivery member in fluid communication with the containment chamber; the containment chamber containing a procoagulating agent; wherein a clotting cascade can be initiated when the blood fluid is placed into the sterile containment chamber and thereby exposed to the procoagulating agent; b) drawing a sufficient amount of an autologous blood fluid from the patient into the containment chamber to provide a sufficient amount of blood fluid to the wound, once the clotting cascade is initiated, to enable the blood fluid to knit together to form a clot, the blood fluid being whole blood; and c) delivering the blood fluid to the wound via the cannulated delivery member subsequent to the initiation of the clotting cascade in the containment chamber so that the clotting cascade can be completed and a clot can fonn within the wound after the blood fluid is delivered to the wound.
Claim 51
Dependent Claim
160 words
A clotting cascade initiation apparatus within which a blood fluid can be received and retained, the blood fluid being useful for treatment of a wound within a patient, said apparatus comprising: a substantially enclosed sterile containment chamber within which the blood fluid can be received and retained, the substantially enclosed sterile containment chamber containing a procoagulating agent; and a cannulated delivery member in fluid communication with the containment chamber such that blood fluid can pass out of the containment chamber and through the cannulated delivery member; wherein a clotting cascade is initiated when the blood fluid is accepted into the sterile containment chamber and exposed to the procoagulating agent within the chamber, and the blood fluid can be delivered to the wound subsequent to the initiation of the clotting cascade via the cannulated delivery member such that the clotting cascade can be completed proximate the wound and a clot can form proximate the wound when the clotting cascade is completed.
Claim 52
Dependent Claim
160 words
A clotting cascade initiation apparatus within which a blood fluid can be received and retained, the blood fluid being useful for treatment of a wound within a patient, said apparatus comprising: a substantially enclosed sterile containment chamber within which the blood fluid can be received and retained, the substantially enclosed sterile containment chamber containing a procoagulating agent; and a cannulated delivery member in fluid communication with the containment chamber such that blood fluid can pass out of the containment chamber and through the cannulated delivery member; wherein a clotting cascade is initiated when the blood fluid is accepted into the sterile containment chamber and exposed to the procoagulating agent within the chamber, and the blood fluid can be delivered to the wound subsequent to the initiation of the clotting cascade via the cannulated delivery member such that the clotting cascade can be completed proximate the wound and a clot can form proximate the wound when the clotting cascade is completed.
Claim 53
Independent Claim
106 words
A method of closing wound in a patient the method consisting essentially of, (a) withdrawing an amount of blood fluid from the patient sufficient to form a clot within a wound of sufficient size to prevent fluid from passing through the wound, the blood fluid being whole blood; (b) exposing the blood fluid to a procoagulating agent to initiate a clotting cascade within the blood fluid; and (c) subsequently returning the blood fluid to the patient proximate the wound such that the blood fluid, in which the clotting cascade has been initiated, will subsequently clot proximate the wound such that fluid cannot pass through the wound.
Claim 54
Independent Claim
106 words
A method of closing wound in a patient the method consisting essentially of, (a) withdrawing an amount of blood fluid from the patient sufficient to form a clot within a wound of sufficient size to prevent fluid from passing through the wound, the blood fluid being whole blood; (b) exposing the blood fluid to a procoagulating agent to initiate a clotting cascade within the blood fluid; and (c) subsequently returning the blood fluid to the patient proximate the wound such that the blood fluid, in which the clotting cascade has been initiated, will subsequently clot proximate the wound such that fluid cannot pass through the wound.
Claim 55
Dependent Claim
23 words
The method of claim 46, wherein the step of exposing the blood fluid includes exposing the blood fluid to an anticoagulant inhibiting agent.
Claim 56
Dependent Claim
25 words
The methods of claims 46 and 47, wherein the step of exposing includes the step of exposing the blood fluid to an anticlot inhibiting agent.
Claim 57
Dependent Claim
25 words
The method of claim 47, wherein the anticoagulant inhibiting agent is an inhibiting agent selected from the group consisting of catalytic and non-catalytic inhibiting agents.
Claim 58
Dependent Claim
12 words
The method of claim 46, wherein the whole blood contains an anticoagulant.
Claim 59
Dependent Claim
154 words
A kit for closing a wound in a patient, the patient containing a blood fluid; said kit comprising a substantially enclosed sterile containment chamber within which the blood fluid can be received and retained, the substantially enclosed sterile containment chamber containing a procoagulating agent; wherein a clotting cascade is initiated when the blood fluid is accepted into the sterile containment chamber and exposed to the procoagulating agent within the chamber, and the blood fluid can be delivered to the wound subsequent to the initiation of the clotting cascade such that the clotting cascade can be completed within the wound and a clot can form proximate the wound when the clotting cascade is completed; and additional apparatus selected from the group consisting of an introducer, guidewire, dilator, obturator, collapsible catheter sheath, Seldinger needle, balloon catheter, infusion catheter, stent, scalpel, suture line, needle, pouch, tray, tray lid, instructions for use, adhesive identification label and sterility indicator.
Claim 60
Dependent Claim
154 words
A kit for closing a wound in a patient, the patient containing a blood fluid; said kit comprising a substantially enclosed sterile containment chamber within which the blood fluid can be received and retained, the substantially enclosed sterile containment chamber containing a procoagulating agent; wherein a clotting cascade is initiated when the blood fluid is accepted into the sterile containment chamber and exposed to the procoagulating agent within the chamber, and the blood fluid can be delivered to the wound subsequent to the initiation of the clotting cascade such that the clotting cascade can be completed within the wound and a clot can form proximate the wound when the clotting cascade is completed; and additional apparatus selected from the group consisting of an introducer, guidewire, dilator, obturator, collapsible catheter sheath, Seldinger needle, balloon catheter, infusion catheter, stent, scalpel, suture line, needle, pouch, tray, tray lid, instructions for use, adhesive identification label and sterility indicator.
Claim 61
Dependent Claim
35 words
The kit of claim 51; further comprising a cannulated delivery member in fluid communication with the containment chamber such that blood fluid can pass out of the containment chamber and through the camulated delivery member.
Claim 62
Dependent Claim
32 words
The kit of claim 52, wherein the substantially sterile containment chamber contains an agent selected from the group consisting of an anticoagulant inhibiting agent, an anticlot inhibiting agent and a combination thereof.
Claim 63
Dependent Claim
19 words
The apparatus of claim 15 or 45, the substantially enclosed sterile containment chamber further containing an anticoagulant inhibiting agent.
Claim 64
Dependent Claim
17 words
The apparatus of claim 54; the substantially enclosed sterile containment chamber further containing an anticlot inhibiting agent.
Claim 65
Dependent Claim
24 words
The apparatus of claim 54, wherein the anticoagulant inhibiting agent is an agent selected from the group consisting of catalytic agents and non-catalytic agents.
Claim 66
Dependent Claim
19 words
The apparatus of claim 15 or 45; the substantially enclosed sterile containment chamber further containing an anticlot inhibiting agent.
Claim 67
Dependent Claim
54 words
The apparatus of claim 15 or 45; constituting a kit when when further comprising additional apparatus selected from the group consisting of an introducer, guidewire, dilator, obturator, collapsible catheter sheath, Seldinger needle, balloon catheter, infusion catheter, stent, scalpel, suture line, needle, pouch, tray, tray lid, instructions for use, adhesive identification label and sterility indicator.
Claim 68
Dependent Claim
27 words
The method of claim 25, 34, 42, or 44, wherein the step of providing includes providing a substantially enclosed sterile containment chamber containing an anticoagulant inhibiting agent.
Claim 69
Dependent Claim
27 words
The methods of claim 25, 34, 42, or 44 wherein the step of providing includes providing a substantially enclosed sterile containment chamber containing an anticlot inhibiting agent.
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