US6147276A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Quiescent cell populations for nuclear transfer in the production of non-human mammals and non-human mammalian embryos
Assignee: Minister of Agriculture Fisheries and Food UK
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Filing: 1997-02-19
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Granted: 2000-11-14
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Expires: 2017-02-19
Abstract
Abstract A method of reconstituting a mammalian embryo involves transferring the nucleus from a quiescent donor cell into a suitable recipient cell. The donor cell is quiescent, in that it is caused to exit from the growth and division cycle at G1 and to arrest in the G0 state. Nuclear transfer may take place by cell fusion. The reconstituted embryo may then give rise to one or more mammals. The invention is useful in the production of transgenic mammals as well as non-transgenics of high genetic merit.
Inventors
- 👤 Keith Henry Stockman Campbell
- 👤 Ian Wilmut
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 04:11
CPC Classifications
A; A01; A01K; A01K67/00; A01K67/027; C; C12; C12N; C12N15/00; C12N15/09; C12N15/87; C12N15/873; C12N15/877; C12N15/8771; A61; A61K; A61K35/00; A61K35/12; A61K35/48; A61K35/54; C12N15/8773
Claims Analysis
Total Claims: 24
Claim 1
Independent Claim
55 words
A method of reconstituting a non-human mammalian embryo, comprising a. transferring the nucleus of a quiescent diploid donor cell into a suitable enucleated recipient cell of the same species, thereby obtaining a reconstituted cell; b. activating the recipient cell before, during or after nuclear transfer; c. incubating the reconstituted cell such that an embryo develops.
Claim 2
Independent Claim
55 words
A method of reconstituting a non-human mammalian embryo, comprising a. transferring the nucleus of a quiescent diploid donor cell into a suitable enucleated recipient cell of the same species, thereby obtaining a reconstituted cell; b. activating the recipient cell before, during or after nuclear transfer; c. incubating the reconstituted cell such that an embryo develops.
Claim 3
Dependent Claim
13 words
The method of claim 1 in which the recipient cell comprises an oocyte.
Claim 4
Dependent Claim
23 words
The method of claim 1 in which the mammal is a selected from the group consisting of: cattle, sheep, pigs, goats and camels.
Claim 5
Dependent Claim
21 words
The method of claim 1 wherein the nucleus of the donor cell is transferred into the recipient cell by cell fusion.
Claim 6
Dependent Claim
20 words
The method of claim 1 wherein the nucleus of the donor cell is transferred into the recipient cell by microinjection.
Claim 7
Dependent Claim
15 words
The method of claim 1 which further comprises splitting the embryo prior to full development.
Claim 8
Dependent Claim
13 words
The method of claim 1 in which the donor nucleus is genetically modified.
Claim 9
Dependent Claim
14 words
The method of claim 1 in which the donor cell is a somatic cell.
Claim 10
Dependent Claim
15 words
The method of claim 8 in which the somatic cell is a differentiated somatic cell.
Claim 11
Dependent Claim
14 words
The method of claim 1 in which the donor cell is a foetal cell.
Claim 12
Dependent Claim
14 words
The method of claim 1 in which the donor cell is an embryonic cell.
Claim 13
Dependent Claim
22 words
The method of claim 11 in which the donor embryonic cell is from an embryo produced by the method of claim 11.
Claim 14
Dependent Claim
12 words
The method of claim 1 in which the mammal is a sheep.
Claim 15
Dependent Claim
12 words
The method of claim 1 in which the mammal is a pig.
Claim 16
Dependent Claim
12 words
The method of claim 11 in which the mammal is a bovine.
Claim 17
Dependent Claim
12 words
The method of claim 11 in which the mammal is a goat.
Claim 18
Dependent Claim
12 words
The method of claim 1 in which the mammal is a rodent.
Claim 19
Dependent Claim
12 words
The method of claim 17 in which the rodent is a rat.
Claim 20
Dependent Claim
12 words
The method of claim 17 in which the rodent is a mouse.
Claim 21
Dependent Claim
12 words
The method of claim 1 in which the mammal is a rabbit.
Claim 22
Dependent Claim
41 words
A method of preparing a non-human mammal, comprising: (a) reconstituting a non-human mammalian embryo according to the method of claim 1; and (b) transferring the embryo to a female of the same species; (c) developing the embryo into the non-human mammal.
Claim 23
Dependent Claim
29 words
A method of producing a cell line, comprising reconstituting a non-human mammalian embryo according to claim 11, isolating cells from the embryo and culturing the cells from the embryo.
Claim 24
Dependent Claim
29 words
A method of producing a cell line, comprising reconstituting a non-human mammalian embryo according to claim 2, isolating cells from the embryo and culturing the cells from the embryo.
References Cited (Backward Citations)
US5011780A
Cited
CA2059199A1
Cited
GB2265909A
Cited
WO1994006422A1
Cited
CA2092258A1
Cited
US5496720A
Cited
WO1995003795A1
Cited
WO1996007732A1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2003-11-14 | 2004-05-15 | 2004-11-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2007-11-14 | 2008-05-15 | 2008-11-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2011-11-14 | 2012-05-15 | 2012-11-14 | $7700.00 | Window Closed / Lapsed |