US6030779A
Expired / Lapsed
US
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Screening for novel bioactivities
Assignee: BP Corp North America Inc
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Filing: 1997-10-06
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Granted: 2000-02-29
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Expires: 2017-10-06
Abstract
Abstract Disclosed is a process for identifying clones having a specified enzyme activity by screening for the specified enzyme activity in a library of clones prepared by (i) selectively isolating target nucleic acid from nucleic acid derived from at least one microorganism, by use of at least one polynucleotide probe comprising at least a portion of a nucleic acid sequence encoding an enzyme having the specified enzyme activity; and (ii) transforming a host with isolated target nucleic acid to produce a library of clones which are screened for the specified enzyme activity.
Inventors
- 👤 Jay M. Short
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 01:42
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/10; C12N15/1034; C12N15/1086; C12N15/102; C12N9/00; C12N9/14; C12N9/16; C12N9/24; C12N9/2402; C12Q; C12Q1/00; C12Q1/68; C12Q1/6811
Claims Analysis
Total Claims: 42
Claim 1
Independent Claim
138 words
A method for identifying a desired activity encoded by a genomic DNA population comprising: (a) obtaining a single-stranded genomic DNA population; (b) contacting the single-stranded DNA population of (a) with a DNA probe tunder conditions and for sufficient time to allow hybridization and to produce a double-stranded complex of probe and members of the genomic DNA population which hybridize thereto; (c) separating the complex from the single-stranded DNA population of (b); (d) releasing from the probe the members of the genomic population which had been bound to the probe; (e) forming double-stranded DNA from the members of the genomic population of (d); (f) introducing the double-stranded DNA of (e) into a suitable host cell to produce an expression library containing a plurality of clones containing the selected DNA; and (g) screening the expression library for the desired activity.
Claim 2
Independent Claim
138 words
A method for identifying a desired activity encoded by a genomic DNA population comprising: (a) obtaining a single-stranded genomic DNA population; (b) contacting the single-stranded DNA population of (a) with a DNA probe tunder conditions and for sufficient time to allow hybridization and to produce a double-stranded complex of probe and members of the genomic DNA population which hybridize thereto; (c) separating the complex from the single-stranded DNA population of (b); (d) releasing from the probe the members of the genomic population which had been bound to the probe; (e) forming double-stranded DNA from the members of the genomic population of (d); (f) introducing the double-stranded DNA of (e) into a suitable host cell to produce an expression library containing a plurality of clones containing the selected DNA; and (g) screening the expression library for the desired activity.
Claim 3
Dependent Claim
17 words
The method of claim 1, wherein the genomic DNA population is derived from uncultivated or cultivated microorganisms.
Claim 4
Dependent Claim
17 words
The method of claim 2, wherein the uncultivated or cultivated microorganisms are isolated from an environmental sample.
Claim 5
Dependent Claim
15 words
The method of claim 3, wherein the microorganisms isolated from an environmental sample are extremophiles.
Claim 6
Dependent Claim
23 words
The method of claim 4, wherein the extremophiles are selected from the group consisting of thermophiles, hyperthermophiles, psychrophiles, halophiles, acidophiles, barophiles and psychrotrophs.
Claim 7
Dependent Claim
20 words
The method of claim 1, wherein the genomic DNA, or fragments thereof, comprise one or more operons, or portions thereof.
Claim 8
Dependent Claim
18 words
The method of claim 6, wherein the operons, or portions thereof, encodes a complete or partial metabolic pathway.
Claim 9
Dependent Claim
21 words
The method of claim 7, wherein the operons or portions thereof encoding a complete or partial metabolic pathway encodes polyketide synthases.
Claim 10
Dependent Claim
31 words
The method of claim 1, wherein the expression library containing a plurality of clones is selected from the group consisting of phage, plasmids, phagemids, cosmids, phosmids, viral vectors and artificial chromosomes.
Claim 11
Dependent Claim
27 words
The method of claim 1, wherein the suitable host cell is selected from the group consisting of a bacterium, fungus, plant cell, insect cell and animal cell.
Claim 12
Dependent Claim
31 words
The method of claim 1, wherein the DNA probe bound to a ligand is comprised of at least a portion of the coding region sequence of DNA for a known bioactivity.
Claim 13
Dependent Claim
28 words
The method of claim 1, wherein the ligand is selected from the group consisting of antigens or haptens, biotin or iminobiotin, sugars, enzymes, apoenzymes homopolymeric oligonucleotides and hormones.
Claim 14
Dependent Claim
38 words
The method of claim 1, wherein the binding partner for said ligand is selected from the group consisting of antibodies or specific binding fragments thereof, avidin or streptavidin, lectins, enzyme inhibitors, apoenzyme cofactors, homopolymeric oligonucleotides and hormone receptors.
Claim 15
Dependent Claim
32 words
The method of claim 1, wherein a solid phase is selected from the group consisting of a glass or polymeric surface, a packed column of polymeric beads or magnetic or paramagnetic particles.
Claim 16
Dependent Claim
15 words
The method of claim 1, further comprising producing a cell-free extract of the expression library.
Claim 17
Dependent Claim
24 words
The method of claim 15, further comprising combining the expression library extract with a host cell-free protein extract from a metabolically rich host organism.
Claim 18
Dependent Claim
11 words
The method of claim 16, wherein the host organism is Streptomyces.
Claim 19
Dependent Claim
11 words
The method of claim 16, wherein the host organism is Bacillus.
Claim 20
Independent Claim
180 words
A method for preselecting a desired DNA from a genomic DNA population comprising: (a) obtaining a single-stranded genomic DNA population; (b) contacting the single-stranded DNA population of (a) with a ligand-bound oligonucleotide probe that is complementary to a secretion signal sequence unique to a given class of proteins under conditions permissive of hybridization to form a double-stranded complex; (c) contacting the double-stranded complex of (a) with a solid phase specific binding partner for said ligand so as to produce a solid phase complex; (d) separating the solid phase complex from the single-stranded DNA population of (a); (e) releasing the members of the genomic population which had bound to said solid phase bound probe; (f) separating the solid phase bound probe from the members of the genomic population which had bound thereto; (g) forming double-stranded DNA from the members of the genomic population of (e); (h) introducing the double-stranded DNA of (g) into a suitable host cell to form an expression library containing a plurality of clones containing the selected DNA; and (i) screening the expression library for the desired activity.
Claim 21
Independent Claim
180 words
A method for preselecting a desired DNA from a genomic DNA population comprising: (a) obtaining a single-stranded genomic DNA population; (b) contacting the single-stranded DNA population of (a) with a ligand-bound oligonucleotide probe that is complementary to a secretion signal sequence unique to a given class of proteins under conditions permissive of hybridization to form a double-stranded complex; (c) contacting the double-stranded complex of (a) with a solid phase specific binding partner for said ligand so as to produce a solid phase complex; (d) separating the solid phase complex from the single-stranded DNA population of (a); (e) releasing the members of the genomic population which had bound to said solid phase bound probe; (f) separating the solid phase bound probe from the members of the genomic population which had bound thereto; (g) forming double-stranded DNA from the members of the genomic population of (e); (h) introducing the double-stranded DNA of (g) into a suitable host cell to form an expression library containing a plurality of clones containing the selected DNA; and (i) screening the expression library for the desired activity.
Claim 22
Dependent Claim
17 words
The method of claim 19, wherein the genomic DNA population is derived from uncultivated or cultivated microorganisms.
Claim 23
Dependent Claim
17 words
The method of claim 20, wherein the uncultivated or cultivated microorganisms are isolated from an environmental sample.
Claim 24
Dependent Claim
15 words
The method of claim 21, wherein the microorganisms isolated from an environmental sample are extremophiles.
Claim 25
Dependent Claim
23 words
The method of claim 22, wherein the extremophiles are selected from the group consisting of thermophiles, hyperthermophiles, psychrophiles, halophiles, acidophiles, barophiles and psychrotrophs.
Claim 26
Dependent Claim
20 words
The method of claim 19, wherein the genomic DNA, or fragments thereof, comprise one or more operons, or portions thereof.
Claim 27
Dependent Claim
18 words
The method of claim 24, wherein the operons, or portions thereof, encodes a complete or partial metabolic pathway.
Claim 28
Dependent Claim
21 words
The method of claim 25, wherein the operons or portions thereof encoding a complete or partial metabolic pathway encodes polyketide synthases.
Claim 29
Dependent Claim
31 words
The method of claim 19, wherein the expression library containing a plurality of clones is selected from the group consisting of phage, plasmids, phagemids, cosmids, phosmids, viral vectors and artificial chromosomes.
Claim 30
Dependent Claim
28 words
The method of claim 19 wherein the a suitable host cell is selected from the group consisting of a bacterium, fungus, plant cell, insect cell and animal cell.
Claim 31
Dependent Claim
31 words
The method of claim 19, wherein the DNA probe bound to a ligand is comprised of at least a portion of the coding region sequence of DNA for a known bioactivity.
Claim 32
Dependent Claim
28 words
The method of claim 19, wherein the ligand is selected from the group consisting of antigens or haptens, biotin or iminobiotin, sugars, enzymes, apoenzymes homopolymeric oligonucleotides and hormones.
Claim 33
Dependent Claim
38 words
The method of claim 19, wherein the binding partner for said ligand is selected from the group consisting of antibodies or specific binding fragments thereof, avidin or streptavidin, lectins, enzyme inhibitors, apoenzyme cofactors, homopolymeric oligonucleotides and hormone receptors.
Claim 34
Dependent Claim
32 words
The method of claim 19, wherein a solid phase is selected from the group consisting of a glass or polymeric surface, a packed column of polymeric beads or magnetic or paramagnetic particles.
Claim 35
Dependent Claim
15 words
The method of claim 19, further comprising producing a cell-free extract of the expression library.
Claim 36
Dependent Claim
23 words
The method of claim 33, firther comprising combining the expression library extract with a cell-free protein extract from a metabolically rich host organism.
Claim 37
Dependent Claim
11 words
The method of claim 34, wherein the host organism is Streptomyces.
Claim 38
Dependent Claim
11 words
The method of claim 34, wherein the host organism is Bacillus.
Claim 39
Independent Claim
81 words
A method for identifying a desired bioactivity or biomolecule comprising: (a) producing one or more expression libraries derived from nucleic acid directly isolated from the environment; (b) combining a cell-free extract from the expression library or libraries with crude or partially purified extracts, or pure proteins from a metabolically rich cell line to form an extract mixture free from the library and cell line cells; and (c) screening said mixture to identify an activity or molecule produced by the extract mixture.
Claim 40
Independent Claim
81 words
A method for identifying a desired bioactivity or biomolecule comprising: (a) producing one or more expression libraries derived from nucleic acid directly isolated from the environment; (b) combining a cell-free extract from the expression library or libraries with crude or partially purified extracts, or pure proteins from a metabolically rich cell line to form an extract mixture free from the library and cell line cells; and (c) screening said mixture to identify an activity or molecule produced by the extract mixture.
Claim 41
Independent Claim
58 words
A method for identifying a desired bioactivity or biomolecule comprising: (a) producing one or more expression libraries containing clones having nucleic acid inserts derived from nucleic acid directly isolated from the environment; (b) transferring said clones into a metabolically rich cell line; and (c) screening said cell line to identify clones having a bioactivity or biomolecule of interest.
Claim 42
Independent Claim
58 words
A method for identifying a desired bioactivity or biomolecule comprising: (a) producing one or more expression libraries containing clones having nucleic acid inserts derived from nucleic acid directly isolated from the environment; (b) transferring said clones into a metabolically rich cell line; and (c) screening said cell line to identify clones having a bioactivity or biomolecule of interest.
References Cited (Backward Citations)
US5783431A
Cited
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 2003-02-28 | 2003-08-30 | 2004-02-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2007-02-28 | 2007-08-30 | 2008-02-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2011-02-28 | 2011-08-30 | 2012-02-29 | $7700.00 | Window Closed / Lapsed |