US6004788A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Enzyme kits and libraries
Assignee: INDUSTRIAL BIOCATALYSIS Inc
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Filing: 1995-07-18
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Granted: 1999-12-21
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Expires: 2015-07-18
Abstract
Abstract Recombinant enzyme libraries and kits where a plurality of enzymes are each characterized by different physical and/or chemical characteristics and classified by common characteristics. The characteristics are determined by screening of recombinant enzymes expressed by a DNA library produced from various microorganisms.
Inventors
- 👤 Jay M. Short
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 00:19
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/10; C12N15/1034; C12N15/1093; C12N15/1086; C12N15/11; C12N15/52; C12Q; C12Q1/00
Claims Analysis
Total Claims: 3
Claim 1
Independent Claim
121 words
A kit comprising at least one container containing a mixture of enzymes, wherein said enzymes are obtained by culturing a gene expression library, comprising a pool of expression constructs, each expression construct comprising a vector containing one or more cDNA or genomic DNA fragments, wherein the cDNA or genomic DNA fragments in the pool of expression constructs are derived from a plurality of species of uncultured donor microorganisms, and wherein the cDNA or genomic DNA fagments are each operably-associated with one or more regulatory regions that drives expression of genes encoded by the cDNA or genomic DNA fragments in an appropriate host organism, wherein the enzyme is selected from the group consisting of oxidoreductase, transferase, hydrolase, lyase, isomerase, and ligase activity.
Claim 2
Dependent Claim
121 words
A kit comprising at least one container containing a mixture of enzymes, wherein said enzymes are obtained by culturing a gene expression library, comprising a pool of expression constructs, each expression construct comprising a vector containing one or more cDNA or genomic DNA fragments, wherein the cDNA or genomic DNA fragments in the pool of expression constructs are derived from a plurality of species of uncultured donor microorganisms, and wherein the cDNA or genomic DNA fagments are each operably-associated with one or more regulatory regions that drives expression of genes encoded by the cDNA or genomic DNA fragments in an appropriate host organism, wherein the enzyme is selected from the group consisting of oxidoreductase, transferase, hydrolase, lyase, isomerase, and ligase activity.
Claim 3
Dependent Claim
14 words
The method of claim 1, wherein the microorganisms are derived from an environmental sample.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2002-12-20 | 2003-06-21 | 2003-12-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2006-12-20 | 2007-06-21 | 2007-12-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2010-12-20 | 2011-06-21 | 2011-12-21 | $7700.00 | Window Closed / Lapsed |