US6001574A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Production and use of normalized DNA libraries
Assignee: BP Corp North America Inc
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Filing: 1998-03-04
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Granted: 1999-12-14
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Expires: 2018-03-04
Abstract
Abstract Disclosed is a process for forming a normalized genomic DNA library from an environmental sample by (a) isolating a genomic DNA population from the environmental sample; (b) at least one of (i) amplifying the copy number of the DNA population so isolated and (ii) recovering a fraction of the isolated genomic DNA having a desired characteristic; and (c) normalizing the representation of various DNAs within the genomic DNA population so as to form a normalized library of genomic DNA from the environmental sample. Also disclosed is a normalized genomic DNA library formed from an environmental sample by the process.
Inventors
- 👤 Jay M. Short
- 👤 Eric J. Mathur
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 12:35
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/10; C12N15/1034; C12N15/1093; C12N15/1096; C12Q; C12Q1/00; C12Q1/68; C12Q1/6811
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
90 words
A process for forming a normalized genomic DNA library from an environmental sample, which comprises: (a) obtaining a genomic DNA population from the environmental sample; (b) at least one of the steps selected from the group consisting of (i) amplifying the copy number of the DNA population so isolated and (ii) recovering a fraction of the isolated genomic DNA having a desired characteristic; and (c) normalizing the representation of various DNAs within the genomic DNA population so as to form a normalized library of genomic DNA from the environmental sample.
Claim 2
Dependent Claim
90 words
A process for forming a normalized genomic DNA library from an environmental sample, which comprises: (a) obtaining a genomic DNA population from the environmental sample; (b) at least one of the steps selected from the group consisting of (i) amplifying the copy number of the DNA population so isolated and (ii) recovering a fraction of the isolated genomic DNA having a desired characteristic; and (c) normalizing the representation of various DNAs within the genomic DNA population so as to form a normalized library of genomic DNA from the environmental sample.
Claim 3
Dependent Claim
22 words
The process of claim 1 which comprises the step of recovering a fraction of the isolated genomic DNA having a desired characteristic.
Claim 4
Dependent Claim
20 words
The process of claim 1 which comprises the step of amplifying the copy number of the DNA population so isolated.
Claim 5
Dependent Claim
17 words
The process of claim 1 wherein the step of amplifying the genomic DNA precedes the normalizing step.
Claim 6
Dependent Claim
17 words
The process of claim 1 wherein the step of normalizing the genomic DNA precedes the amplifying step.
Claim 7
Dependent Claim
36 words
The process of claim 1 which comprises both the steps of (i) amplifying the copy number of the DNA population so isolated and (ii) recovering a fraction of the isolated genomic DNA having a desired characteristic.
Claim 8
Dependent Claim
81 words
A normalized genomic DNA library formed from an environmental sample by a process comprising: (a) obtaining a genomic DNA population from the environmental sample; (b) at least one of (i) amplifying the copy number of the DNA population so isolated and (ii) recovering a fraction of the isolated genomic DNA having a desired characteristic; and (c) normalizing the representation of various DNAs within the genomic DNA population so as to form a normalized library of genomic DNA from the environmental sample.
Claim 9
Dependent Claim
81 words
A normalized genomic DNA library formed from an environmental sample by a process comprising: (a) obtaining a genomic DNA population from the environmental sample; (b) at least one of (i) amplifying the copy number of the DNA population so isolated and (ii) recovering a fraction of the isolated genomic DNA having a desired characteristic; and (c) normalizing the representation of various DNAs within the genomic DNA population so as to form a normalized library of genomic DNA from the environmental sample.
Claim 10
Dependent Claim
28 words
The library of claim 1 wherein the process of forming said library comprises the step of recovering a fraction of the isolated genomic DNA having a desired characteristic.
Claim 11
Dependent Claim
26 words
The library of claim 1 wherein the process of forming said library comprises the step of amplifying the copy number of the DNA population so isolated.
Claim 12
Dependent Claim
24 words
The library of claim 1 wherein in the process of forming said library the step of amplifying the genomic DNA precedes the normalizing step.
Claim 13
Dependent Claim
24 words
The library of claim 1 wherein in the process of forming said library the step of normalizing the genomic DNA precedes the amplifying step.
Claim 14
Dependent Claim
42 words
The library of claim 1 wherein the process of forming said library comprises both the steps of (i) amplifying the copy number of the DNA population so isolated and (ii) recovering a fraction of the isolated genomic DNA having a desired characteristic.
Claim 15
Dependent Claim
84 words
A process for forming a normalized library of genomic gene clusters from an environmental sample which comprises: (a) obtaining a genomic DNA population from the environmental sample; (b) at least one of (i) amplifying the copy number of the DNA population so isolated and (ii) recovering a fraction of the isolated genomic DNA having a desired characteristic; and (c) normalizing the representation of various DNAs within the genomic DNA population so as to form a normalized library of genomic DNA from the environmental sample.
Claim 16
Dependent Claim
84 words
A process for forming a normalized library of genomic gene clusters from an environmental sample which comprises: (a) obtaining a genomic DNA population from the environmental sample; (b) at least one of (i) amplifying the copy number of the DNA population so isolated and (ii) recovering a fraction of the isolated genomic DNA having a desired characteristic; and (c) normalizing the representation of various DNAs within the genomic DNA population so as to form a normalized library of genomic DNA from the environmental sample.
Claim 17
Dependent Claim
83 words
A normalized library of genomic gene clusters formed from an environmental sample by a process comprising: (a) obtaining a genomic DNA population from the environmental sample; (b) at least one of (i) amplifying the copy number of the DNA population so isolated and (ii) recovering a fraction of the isolated genomic DNA having a desired characteristic; and (c) normalizing the representation of various DNAs within the genomic DNA population so as to form a normalized library of genomic DNA from the environmental sample.
Claim 18
Dependent Claim
83 words
A normalized library of genomic gene clusters formed from an environmental sample by a process comprising: (a) obtaining a genomic DNA population from the environmental sample; (b) at least one of (i) amplifying the copy number of the DNA population so isolated and (ii) recovering a fraction of the isolated genomic DNA having a desired characteristic; and (c) normalizing the representation of various DNAs within the genomic DNA population so as to form a normalized library of genomic DNA from the environmental sample.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2002-12-13 | 2003-06-14 | 2003-12-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2006-12-13 | 2007-06-14 | 2007-12-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2010-12-13 | 2011-06-14 | 2011-12-14 | $7700.00 | Window Closed / Lapsed |