US5976826A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Device containing cytophilic islands that adhere cells separated by cytophobic regions
Assignee: Boston Children's Hospital
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Filing: 1997-10-16
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Granted: 1999-11-02
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Expires: 2017-10-16
Abstract
Abstract A device is provided for adhering cells in a specific and predetermined position. The device comprises a plate defining a surface and a plurality of cytophilic islands that adhere cells which are isolated by cytophobic regions to which cells do not adhere and further is contiguous with the cytophilic islands. The islands or the regions or both may be formed of a self-assembled monolayer (SAM). Further, the cytophobic regions are wide enough such that less than 10 percent of the cells adhered to the cytophilic islands are allowed to form bridges across the cytophobic regions and contact each other. The device is used in a method for culturing cells on a surface or in a medium and also for performing cytometry. Furthermore, the device is used in immobilization of cells at a surface and for controlling the shape of a cell.
Inventors
- 👤 Rahul Singhvi
- 👤 Amit Kumar
- 👤 George M. Whitesides
- 👤 Donald E. Ingber
- 👤 Gabriel P. Lopez
- 👤 Daniel I. C. Wang
- 👤 Gregory N. Stephanopoulos
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 01:34
CPC Classifications
B; B01; B01J; B01J19/00; B01J19/0046; C; C12; C12N; C12N1/00; B81; B81C; B81C1/00; B81C1/00015; B81C1/00206; B82; B82Y; B82Y30/00; B82Y5/00; C12N5/00; C12N5/0068; C12N5/06; C12N5/0602; C12N5/067; C12Q; C12Q1/00; C12Q1/02; B01J2219/00; B01J2219/00274; B01J2219/00277; B01J2219/00351; B01J2219/00382; B01J2219/00385; B01J2219/00583; B01J2219/00585; B01J2219/00596; B01J2219/00603; B01J2219/00605; B01J2219/0061; B01J2219/00612; B01J2219/00614; B01J2219/00617; B01J2219/00619; B01J2219/00621; B01J2219/00623; B01J2219/00626; B01J2219/0063; B01J2219/00632; B01J2219/00637; B01J2219/00659; B01J2219/00718; B01J2219/0072; B81B; B81B2201/00; B81B2201/02; B81B2201/0214; B81C2201/00; B81C2201/03; B81C2201/034; C12N2535/00; C12N2535/10; C40; C40B; C40B60/00; C40B60/14
Claims Analysis
Total Claims: 35
Claim 1
Independent Claim
92 words
A device for adhering at least one cell in a specific and predetermined pattern comprising: a plate defining a surface, and a plurality of cytophilic islands that adhere cells on said surface isolated by cytophobic regions to which cells do not adhere contiguous with said cytophilic islands, wherein said cytophilic islands are formed of a self-assembled monolayer and said cytophobic regions are sufficiently wide to prevent cells adhered to said cytophilic islands from contacting each other except via formation of cellular bridges above and free of adhesive contact with said cytophobic regions.
Claim 2
Independent Claim
92 words
A device for adhering at least one cell in a specific and predetermined pattern comprising: a plate defining a surface, and a plurality of cytophilic islands that adhere cells on said surface isolated by cytophobic regions to which cells do not adhere contiguous with said cytophilic islands, wherein said cytophilic islands are formed of a self-assembled monolayer and said cytophobic regions are sufficiently wide to prevent cells adhered to said cytophilic islands from contacting each other except via formation of cellular bridges above and free of adhesive contact with said cytophobic regions.
Claim 3
Dependent Claim
16 words
A device as in claim 1, wherein the islands are between 1 and 2,500 square microns.
Claim 4
Dependent Claim
16 words
A device as in claim 1, wherein the islands are between 1 and 500 square microns.
Claim 5
Dependent Claim
16 words
A device as in claim 1, wherein the islands are between 1 and 100 square microns.
Claim 6
Dependent Claim
19 words
A device as in claim 1, wherein the islands have a lateral dimension of between 0.2 and 10 microns.
Claim 7
Dependent Claim
11 words
A device as in claim 1, wherein the islands are elongated.
Claim 8
Dependent Claim
14 words
A device as in claim 1, wherein the islands bind only selected cell types.
Claim 9
Dependent Claim
16 words
A device as in claim 1, wherein the cytophobic regions are formed of a self-assembled monolayer.
Claim 10
Independent Claim
87 words
A device for adhering at least one cell in a specific and predetermined pattern comprising: a plate defining a surface, and a plurality of cytophilic islands that adhere cells on said surface isolated by cytophobic regions to which cells do not adhere formed of a self-assembled monolayer contiguous with said cytophilic islands, wherein said cytophobic regions are sufficiently wide to prevent cells adhered to said cytophilic islands from contacting each other except via formation of cellular bridges above and free of adhesive contact with said cytophobic regions.
Claim 11
Independent Claim
87 words
A device for adhering at least one cell in a specific and predetermined pattern comprising: a plate defining a surface, and a plurality of cytophilic islands that adhere cells on said surface isolated by cytophobic regions to which cells do not adhere formed of a self-assembled monolayer contiguous with said cytophilic islands, wherein said cytophobic regions are sufficiently wide to prevent cells adhered to said cytophilic islands from contacting each other except via formation of cellular bridges above and free of adhesive contact with said cytophobic regions.
Claim 12
Dependent Claim
16 words
A device as in claim 9, wherein the islands are between 1 and 2,500 square microns.
Claim 13
Dependent Claim
16 words
A device as in claim 9, wherein the islands are between 1 and 500 square microns.
Claim 14
Dependent Claim
16 words
A device as in claim 9, wherein the islands are between 1 and 100 square microns.
Claim 15
Dependent Claim
19 words
A device as in claim 9, wherein the islands have a lateral dimension of between 0.2 and 10 microns.
Claim 16
Dependent Claim
11 words
A device as in claim 9, wherein the islands are elongated.
Claim 17
Dependent Claim
14 words
A device as in claim 9, wherein the islands bind only selected cell types.
Claim 18
Dependent Claim
121 words
In a method for culturing cells on a surface or in a medium on a surface, the improvement comprising: providing a plate defining a surface and a plurality of cytophilic islands that adhere cells on the surface isolated by cytophobic regions to which cells do not adhere contiguous with said cytophilic islands, wherein said cytophilic islands are formed of a self-assembled monolayer and said cytophobic regions are sufficiently wide to prevent cells adhered to said cytophilic islands from contacting each other except via formation of cellular bridges above and free of adhesive contact with said cytophobic regions; and culturing the cells on one or more of the cytophilic islands or in a medium on a said one or more cytophilic islands.
Claim 19
Dependent Claim
121 words
In a method for culturing cells on a surface or in a medium on a surface, the improvement comprising: providing a plate defining a surface and a plurality of cytophilic islands that adhere cells on the surface isolated by cytophobic regions to which cells do not adhere contiguous with said cytophilic islands, wherein said cytophilic islands are formed of a self-assembled monolayer and said cytophobic regions are sufficiently wide to prevent cells adhered to said cytophilic islands from contacting each other except via formation of cellular bridges above and free of adhesive contact with said cytophobic regions; and culturing the cells on one or more of the cytophilic islands or in a medium on a said one or more cytophilic islands.
Claim 20
Dependent Claim
19 words
In a method as in claim 16, the improvement wherein the cytophobic regions are formed of a self-assembled monolayer.
Claim 21
Dependent Claim
138 words
In a method for performing cytometry that includes assaying the numbers or ratios of different cell types in a sample, the improvement comprising: providing a plate defining a surface and a plurality of cytophilic islands that adhere cells on the surface isolated by cytophobic regions to which cells do not adhere contiguous with said cytophilic islands, wherein said cytophilic islands are formed of a self-assembled monolayer and said cytophobic regions are sufficiently wide to prevent cells adhered to said cytophilic islands from contacting each other except via formation of cellular bridges above and free of adhesive contact with said cytophobic regions; contacting a suspension of cells with the plate for a period of time sufficient to allow a cell to bind to one of said plurality of cytophilic islands; and detecting the cell bound to the cytophilic island.
Claim 22
Dependent Claim
138 words
In a method for performing cytometry that includes assaying the numbers or ratios of different cell types in a sample, the improvement comprising: providing a plate defining a surface and a plurality of cytophilic islands that adhere cells on the surface isolated by cytophobic regions to which cells do not adhere contiguous with said cytophilic islands, wherein said cytophilic islands are formed of a self-assembled monolayer and said cytophobic regions are sufficiently wide to prevent cells adhered to said cytophilic islands from contacting each other except via formation of cellular bridges above and free of adhesive contact with said cytophobic regions; contacting a suspension of cells with the plate for a period of time sufficient to allow a cell to bind to one of said plurality of cytophilic islands; and detecting the cell bound to the cytophilic island.
Claim 23
Dependent Claim
20 words
In a method as recited in claim 18, the improvement wherein the cytophobic regions are formed of a self-assembled monolayer.
Claim 24
Dependent Claim
118 words
In a method for immobilizing a cell at a surface, the improvement comprising: providing a plate defining a surface and a plurality of cytophilic islands that adhere cells on the surface isolated by cytophobic regions to which cells do not adhere contiguous with said cytophilic islands, wherein said cytophilic islands are formed of a self-assembled monolayer and said cytophobic regions are sufficiently wide to prevent cells adhered to said cytophilic islands from contacting each other except via formation of cellular bridges above and free of adhesive contact with said cytophobic regions; and exposing the plate to a cell for a period of time sufficient to allow the cell to bind to one of said plurality of cytophilic islands.
Claim 25
Dependent Claim
118 words
In a method for immobilizing a cell at a surface, the improvement comprising: providing a plate defining a surface and a plurality of cytophilic islands that adhere cells on the surface isolated by cytophobic regions to which cells do not adhere contiguous with said cytophilic islands, wherein said cytophilic islands are formed of a self-assembled monolayer and said cytophobic regions are sufficiently wide to prevent cells adhered to said cytophilic islands from contacting each other except via formation of cellular bridges above and free of adhesive contact with said cytophobic regions; and exposing the plate to a cell for a period of time sufficient to allow the cell to bind to one of said plurality of cytophilic islands.
Claim 26
Dependent Claim
19 words
In a method as in claim 20, the improvement wherein the cytophobic regions are formed of a self-assembled monolayer.
Claim 27
Dependent Claim
15 words
A method as in claim 20, the improvement further comprising performing microinjection on the cell.
Claim 28
Dependent Claim
15 words
A method as in claim 20, the improvement further comprising performing microinjection on the cell.
Claim 29
Independent Claim
137 words
A method for controlling the shape of a cell, comprising: providing a plate defining a surface and a plurality of cytophilic islands that adhere cells on the surface isolated by cytophobic regions to which cells do not adhere contiguous with said cytophilic islands, wherein said cytophilic islands are formed of a self-assembled monolayer and said cytophobic regions are sufficiently wide to prevent cells adhered to said cytophilic islands from contacting each other except via formation of cellular bridges above and free of adhesive contact with said cytophobic region; contacting one of said plurality of cytophilic islands with a cell for a period of time sufficient to allow the cell to adhere to the cytophilic island; and growing the cell on the cytophilic island while allowing the shape of the island to influence the shape of the cell.
Claim 30
Independent Claim
137 words
A method for controlling the shape of a cell, comprising: providing a plate defining a surface and a plurality of cytophilic islands that adhere cells on the surface isolated by cytophobic regions to which cells do not adhere contiguous with said cytophilic islands, wherein said cytophilic islands are formed of a self-assembled monolayer and said cytophobic regions are sufficiently wide to prevent cells adhered to said cytophilic islands from contacting each other except via formation of cellular bridges above and free of adhesive contact with said cytophobic region; contacting one of said plurality of cytophilic islands with a cell for a period of time sufficient to allow the cell to adhere to the cytophilic island; and growing the cell on the cytophilic island while allowing the shape of the island to influence the shape of the cell.
Claim 31
Dependent Claim
15 words
A method as in claim 24, further comprising influencing or observing differentiation of the cell.
Claim 32
Dependent Claim
16 words
A method as in claim 24, wherein the cytophobic regions are formed of a self-assembled monolayer.
Claim 33
Dependent Claim
28 words
A method as in claim 24, wherein said one of said plurality of cytophilic islands is of a size sufficient to allow one cell only to adhere thereto.
Claim 34
Dependent Claim
41 words
A method as in claim 24, wherein said one of said plurality of cytophilic islands is of a predetermined shape, the method further comprising observing the effect on cell differentiation of adhering the cell to the cytophilic island of predetermined shape.
Claim 35
Dependent Claim
29 words
A method as in claim 24, wherein said one of said plurality of cytophilic islands is of a size sufficient to allow a plurality of cells to bind thereto.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2002-11-01 | 2003-05-03 | 2003-11-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2006-11-01 | 2007-05-03 | 2007-11-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2010-11-01 | 2011-05-03 | 2011-11-02 | $7700.00 | Window Closed / Lapsed |