US5962221A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Oligonucleotide constructs and methods for the generation of sequence signatures from nucleic acids
Assignee: University of Tennessee Research Foundation
•
Filing: 1995-06-09
•
Granted: 1999-10-05
•
Expires: 2015-06-09
Abstract
Abstract Novel oligonucleotides for amplification and profiling of nucleic acid templates are disclosed. Enhancements of nucleic acid fingerprinting methods are disclosed.
Inventors
- 👤 Gustavo Caetano-Anolles
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 12:20
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6876; C12Q1/6813; C12Q1/6827; C12Q1/6834; C12Q1/6837; C12Q1/6844; C12Q1/6858; C12Q1/686; C12Q2600/00; C12Q2600/166; Y; Y10; Y10S; Y10S435/00; Y10S435/81
Claims Analysis
Total Claims: 44
Claim 1
Independent Claim
96 words
An oligonucleotide construct comprising a structure selected from the group consisting of (B x ) n (N) m and (N) m (B x ) n , wherein B is A, T, G, or C and wherein each B may be the same as or different than any other B in the construct so long as at least one B differs from another B, x is a whole number from 2 to 5, n is a whole number from 2 to 10, N is a degenerate base, and m is a whole number from 1 to 10.
Claim 2
Dependent Claim
96 words
An oligonucleotide construct comprising a structure selected from the group consisting of (B x ) n (N) m and (N) m (B x ) n , wherein B is A, T, G, or C and wherein each B may be the same as or different than any other B in the construct so long as at least one B differs from another B, x is a whole number from 2 to 5, n is a whole number from 2 to 10, N is a degenerate base, and m is a whole number from 1 to 10.
Claim 3
Dependent Claim
11 words
The construct of claim 1 wherein n is 3 to 6.
Claim 4
Dependent Claim
11 words
The construct of claim 1 wherein m is 2 to 5.
Claim 5
Dependent Claim
11 words
The construct of claim 3 wherein m is 2 or 3.
Claim 6
Dependent Claim
79 words
The construct of claim 1 which further comprises an arbitrary sequence having the formula (R) y , wherein R is selected from the group consisting of C, G, T, and A, and y is a whole number from 1 to 25, and which arbitrary sequence (R) y is connected to (B x ) n , (N) m , or both (B x ) n and (N) m , or is within (B x ) n or (N) m .
Claim 7
Dependent Claim
11 words
The construct of claim 5 wherein y is 2 to 10.
Claim 8
Dependent Claim
85 words
An oligonucleotide mini-hairpin primer having a 3' single stranded end and a 5' end which primer comprises a double stranded stem of at least 2 complementary annealed base pairs, the double stranded stem extending to the 5' end of the primer and the other end of the stem extending as the single strand of at least 3 bases to the 3' end of the primer, and intermediate to the 5' and 3' ends of the stem, a single stranded loop of at least 3 bases.
Claim 9
Dependent Claim
85 words
An oligonucleotide mini-hairpin primer having a 3' single stranded end and a 5' end which primer comprises a double stranded stem of at least 2 complementary annealed base pairs, the double stranded stem extending to the 5' end of the primer and the other end of the stem extending as the single strand of at least 3 bases to the 3' end of the primer, and intermediate to the 5' and 3' ends of the stem, a single stranded loop of at least 3 bases.
Claim 10
Dependent Claim
14 words
The primer of claim 7 wherein the loop consists of 3 or 4 bases.
Claim 11
Dependent Claim
13 words
The primer of claim 7 wherein the stem consists of 2 base pairs.
Claim 12
Dependent Claim
14 words
The primer of claim 7 wherein the base pairs are only GC base pairs.
Claim 13
Dependent Claim
15 words
The primer of claim 7 wherein the single strand consists of 3 to 8 bases.
Claim 14
Dependent Claim
82 words
An oligonucleotide primer having a 3' single stranded end and a 5' end which primer comprises a double stranded stem of 2 complementary annealed base pairs, the double stranded stem extending to the 5' end of the primer and the other end of the stem extending as the single strand of at least 3 bases to the 3' end of the primer, and intermediate to the 5' and 3' ends of the stem, a single stranded loop of 3 or 4 bases.
Claim 15
Dependent Claim
82 words
An oligonucleotide primer having a 3' single stranded end and a 5' end which primer comprises a double stranded stem of 2 complementary annealed base pairs, the double stranded stem extending to the 5' end of the primer and the other end of the stem extending as the single strand of at least 3 bases to the 3' end of the primer, and intermediate to the 5' and 3' ends of the stem, a single stranded loop of 3 or 4 bases.
Claim 16
Dependent Claim
12 words
The oligonucleotide primer of claim 12 wherein the loop has 3 bases.
Claim 17
Dependent Claim
12 words
The oligonucleotide primer of claim 12 wherein the loop has 4 bases.
Claim 18
Dependent Claim
16 words
The oligonucleotide primer of claim 12 wherein one of the bases of the loop is A.
Claim 19
Dependent Claim
19 words
The oligonucleotide primer of claim 12 wherein the length of the single strand is of 3 to 8 nucleotides.
Claim 20
Dependent Claim
11 words
The oligonucleotide primer of claim 12 which is a deoxyribonucleotide sequence.
Claim 21
Dependent Claim
11 words
The oligonucleotide primer of claim 12 which is a ribonucleotide sequence.
Claim 22
Dependent Claim
16 words
The oligonucleotide primer of claim 12 wherein the double stranded stem has two complementary GC pairs.
Claim 23
Dependent Claim
16 words
The oligonucleotide primer of claim 13 wherein the nucleotides of the loop are GAA or GTT.
Claim 24
Dependent Claim
14 words
The oligonucleotide primer of claim 18 wherein the nucleotides of the loop are GAAA.
Claim 25
Dependent Claim
37 words
The oligonucleotide primer of claim 14 which is a DNA primer, and wherein the loop comprises a nucleotide sequence selected from the group consisting of GAA, GTTA, and GNAA, wherein N is A, G, C, and T.
Claim 26
Dependent Claim
22 words
The oligonucleotide primer of claim 22 wherein the loop comprises the nucleotide sequence GNAA, wherein N is G, A, C, and T.
Claim 27
Dependent Claim
36 words
The oligonucleotide primer of claim 13 wherein the single strand comprises a sequence of nucleotides selected from the group consisting of CTG, GCTG, NCTG, GAGCTG, NAGCTG, and CCGAGCTG, wherein N is A, G, C, and T.
Claim 28
Dependent Claim
10 words
The oligonucleotide primer of claim 24 wherein N is G.
Claim 29
Dependent Claim
22 words
The oligonucleotide primer of claim 14 wherein the single strand has a sequence selected from the group consisting of GCTG and GAGCTG.
Claim 30
Dependent Claim
14 words
The oligonucleotide primer of claim 12 wherein the primer has an extended 5' end.
Claim 31
Dependent Claim
22 words
The oligonucleotide primer of claim 12 wherein the primer has a sequence selected from the group consisting of GCGAAGCGCTG, GCGAAGCGAGCTG, and GCGAAGCCCGAGCTG.
Claim 32
Dependent Claim
37 words
The oligonucleotide primer of claim 12 wherein one or more nucleotides at the 3' or 5' end of the primer has been replaced by a base selected from the group consisting of hypoxanthine, 6H,8H03,4-dihydropyrimido(4,5-c) (1,2)oxazin-7-one, and 2-amino-6-methoxyaminopurine.
Claim 33
Dependent Claim
68 words
A combination of a multiplicity of the oligonucleotide primers of claim 12, wherein the 3' single stranded end extending from the double stranded stem comprises 3 nucleotides, wherein the nucleotides constitute combinations of the known 4 bases of DNA, and wherein the portion of the oligonucleotide primers other than the 3' single stranded end extending from the double stranded stem contain identical sequences in each of the primers.
Claim 34
Dependent Claim
79 words
An oligonucleotide primer having a 3' single stranded end and a 5' end which primer comprises a double stranded stem of at least 2 complementary annealed base pairs, the double stranded stem extending to the 5' end of the primer and the other end of the stem extending as the single strand of at least 3 bases to the 3' end of the primer, and intermediate to the 5' and 3' ends of the stem, a single stranded loop.
Claim 35
Dependent Claim
79 words
An oligonucleotide primer having a 3' single stranded end and a 5' end which primer comprises a double stranded stem of at least 2 complementary annealed base pairs, the double stranded stem extending to the 5' end of the primer and the other end of the stem extending as the single strand of at least 3 bases to the 3' end of the primer, and intermediate to the 5' and 3' ends of the stem, a single stranded loop.
Claim 36
Dependent Claim
12 words
The primer of claim 12 which is annealed to a DNA template.
Claim 37
Dependent Claim
20 words
The primer of claim 12 wherein the first three bases of the 3' end are determinative to produce reproducible fingerprints.
Claim 38
Dependent Claim
18 words
The primer of claim 33 wherein the three bases at the 3' end of the primer are arbitrary.
Claim 39
Dependent Claim
23 words
The oligonucleotide primer of claim 31 wherein the loop has 3 or 4 bases, wherein at least one of the bases is A.
Claim 40
Dependent Claim
79 words
An oligonucleotide primer having a 3' single stranded end and a 5' end which primer comprises a double stranded stem of at least 2 complementary annealed base pairs, the double stranded stem extending to the 5' end of the primer and the other end of the stem extending as the single strand of at least 3 bases to the 3' end of the primer, and intermediate to the 5' and 3' ends of the stem, a single stranded loop.
Claim 41
Dependent Claim
79 words
An oligonucleotide primer having a 3' single stranded end and a 5' end which primer comprises a double stranded stem of at least 2 complementary annealed base pairs, the double stranded stem extending to the 5' end of the primer and the other end of the stem extending as the single strand of at least 3 bases to the 3' end of the primer, and intermediate to the 5' and 3' ends of the stem, a single stranded loop.
Claim 42
Dependent Claim
20 words
The primer of claim 36 wherein the first three bases of the 3' end are determinative to produce reproducible fingerprints.
Claim 43
Dependent Claim
18 words
The primer of claim 37 wherein the three bases at the 3' end of the primer are arbitrary.
Claim 44
Dependent Claim
23 words
The oligonucleotide primer of claim 36 wherein the loop has 3 or 4 bases, wherein at least one of the bases is A.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2002-10-04 | 2003-04-05 | 2003-10-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2006-10-04 | 2007-04-05 | 2007-10-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2010-10-04 | 2011-04-05 | 2011-10-05 | $7700.00 | Window Closed / Lapsed |