US5958751A
Expired / Lapsed
US
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α-galactosidase
Assignee: BASF Enzymes LLC
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Filing: 1996-03-08
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Granted: 1999-09-28
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Expires: 2016-03-08
Abstract
Abstract A thermostable alpha-glycosidase derived from various Thermococcus, alcaliphilus AEDII12RA is disclosed. The enzymes are produced from native or recombinant host cells and can be utilized in the food processing industry.
Inventors
- 👤 Dennis Murphy
- 👤 John Reid
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 23:28
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/24; C12N9/2402; C12N9/2465
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
44 words
A substantially purified enzyme selected from the group consisting of: (a) an enzyme comprising the amino acid sequence as set forth in SEQ ID NO:4; and (b) an enzyme comprising 30 contiguous amino acids from SEQ ID NO:4 wherein said enzyme has α-galactosidase activity.
Claim 2
Dependent Claim
44 words
A substantially purified enzyme selected from the group consisting of: (a) an enzyme comprising the amino acid sequence as set forth in SEQ ID NO:4; and (b) an enzyme comprising 30 contiguous amino acids from SEQ ID NO:4 wherein said enzyme has α-galactosidase activity.
Claim 3
Independent Claim
33 words
A method for hydrolyzing α-galactose bonds comprising: contacting a compound having α-galactose bonds with an effective amount of a substantially purified enzyme having the amino acid sequence set forth in SEQ ID NO:4.
Claim 4
Independent Claim
33 words
A method for hydrolyzing α-galactose bonds comprising: contacting a compound having α-galactose bonds with an effective amount of a substantially purified enzyme having the amino acid sequence set forth in SEQ ID NO:4.
Claim 5
Dependent Claim
15 words
An isolated polynucleotide encoding the amino acid sequence as set forth in SEQ ID NO:4.
Claim 6
Dependent Claim
15 words
An isolated polynucleotide encoding the amino acid sequence as set forth in SEQ ID NO:4.
Claim 7
Dependent Claim
59 words
An isolated polynucleotide encoding an enzyme having α-galactosidase activity selected from the group consisting of: a) SEQ ID NO:3; b) SEQ ID NO:3, wherein T can also be U; c) nucleic acid sequences complementary to SEQ ID NO:3; and a polynucleotide encoding an enzyme comprising 30 contiguous amino acids from SEQ ID NO:4 wherein said enzyme has α-galactosidase activity.
Claim 8
Dependent Claim
59 words
An isolated polynucleotide encoding an enzyme having α-galactosidase activity selected from the group consisting of: a) SEQ ID NO:3; b) SEQ ID NO:3, wherein T can also be U; c) nucleic acid sequences complementary to SEQ ID NO:3; and a polynucleotide encoding an enzyme comprising 30 contiguous amino acids from SEQ ID NO:4 wherein said enzyme has α-galactosidase activity.
Claim 9
Dependent Claim
9 words
An expression vector containing a polynucleotide of claim 3.
Claim 10
Dependent Claim
12 words
The expression vector of claim 5, wherein the vector is a plasmid.
Claim 11
Dependent Claim
13 words
The expression vector of claim 5, wherein the vector is a viral vector.
Claim 12
Dependent Claim
12 words
The polynucleotide of claim 3, wherein the polynucleotide sequence is from Thermococcus.
Claim 13
Dependent Claim
11 words
A host cell transformed with the expression vector of claim 5.
Claim 14
Dependent Claim
13 words
The host cell of claim 9, wherein the cell is a eukaryotic cell.
Claim 15
Dependent Claim
13 words
The host cell of claim 9, wherein the cell is a prokaryotic cell.
Claim 16
Dependent Claim
29 words
A method of producing an α-galactosidase polypeptide comprising: a) transforming a host cell with a polynucleotide of claim 3; b) expressing the polynucleotide; and c) recovering the α-galactosidase polypeptide.
Claim 17
Dependent Claim
13 words
The method of claim 12, wherein the host cell is a prokaryotic cell.
Claim 18
Dependent Claim
13 words
The method of claim 12, wherein the host cell is a eukaryotic cell.
Claim 19
Dependent Claim
16 words
A substantially purified polypeptide having the amino acid sequences as set forth in SEQ ID NO:4.
Claim 20
Dependent Claim
16 words
A substantially purified polypeptide having the amino acid sequences as set forth in SEQ ID NO:4.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2002-09-27 | 2003-03-29 | 2003-09-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2006-09-27 | 2007-03-29 | 2007-09-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2010-09-27 | 2011-03-29 | 2011-09-28 | $7700.00 | Window Closed / Lapsed |