US5958672A
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Protein activity screening of clones having DNA from uncultivated microorganisms
Assignee: BP Corp North America Inc
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Filing: 1996-06-03
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Granted: 1999-09-28
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Expires: 2016-06-03
Abstract
Abstract Disclosed is a process of screening clones having DNA from an uncultivated microorganism for a specified protein, e.g. enzyme, activity by screening for a specified protein, e.g. enzyme, activity in a library of clones prepared by (i) recovering DNA from a DNA population derived from at least one uncultivated microorganism; and (ii) transforming a host with recovered DNA to produce a library of clones which is screened for the specified protein, e.g. enzyme, activity.
Inventors
- 👤 Jay M. Short
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 03:57
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/10; C12N15/1034; C12N15/1086; C12N15/1079; C12N15/1093; C12N15/11; C12N15/52; C12Q; C12Q1/00
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
107 words
A method for identifying a protein activity of interest comprising: culturing a gene expression library comprising a pool of expression constructs, each expression construct comprising a vector containing one or more cDNA or genomic DNA fragments, wherein the cDNA or genomic DNA fragments in the pool of expression constructs are derived from a plurality of species of donor organisms, and wherein the cDNA or genomic DNA fragments are each operably-associated with one or more regulatory regions that drives expression of genes encoded by the cDNA or genomic DNA fragments in an appropriate host organism; and detecting the protein activity encoded by the cDNA or genomic DNA fragments.
Claim 2
Independent Claim
107 words
A method for identifying a protein activity of interest comprising: culturing a gene expression library comprising a pool of expression constructs, each expression construct comprising a vector containing one or more cDNA or genomic DNA fragments, wherein the cDNA or genomic DNA fragments in the pool of expression constructs are derived from a plurality of species of donor organisms, and wherein the cDNA or genomic DNA fragments are each operably-associated with one or more regulatory regions that drives expression of genes encoded by the cDNA or genomic DNA fragments in an appropriate host organism; and detecting the protein activity encoded by the cDNA or genomic DNA fragments.
Claim 3
Dependent Claim
13 words
The method of claim 1, wherein the protein activity is an enzymatic activity.
Claim 4
Dependent Claim
24 words
The method of claim 2, wherein the enzymatic activity is selected from the group consisting of oxidoreductase, transferase, hydrolase, lyase, isomerase, and ligase activity.
Claim 5
Dependent Claim
11 words
The method of claim 1, wherein the donor organisms are microorganisms.
Claim 6
Dependent Claim
14 words
The method of claim 4, wherein the microorganisms are derived from an environmental sample.
Claim 7
Dependent Claim
15 words
The method of claim 4, wherein the microorganisms are a mixed population of uncultured organisms.
Claim 8
Dependent Claim
17 words
The method of claim 1, wherein the DNA fragment comprises one or more operons, or portions thereof.
Claim 9
Dependent Claim
18 words
The method of claim 7, wherein the operon or portions thereof encodes a complete or partial metabolic pathway.
Claim 10
Independent Claim
107 words
A method for identifying a protein activity of interest comprising: culturing a gene expression library, comprising a pool of expression constructs, each expression construct comprising a vector containing one or more cDNA or genomic DNA fragments, wherein the cDNA or genomic DNA fragments in the pool of expression constructs are derived from a plurality of species of donor microorganisms, and wherein the cDNA or genomic DNA fragments are each operably-associated with one or more regulatory regions that drives expression of genes encoded by the cDNA or genomic DNA fragments in an appropriate host organism; and detecting the protein activity encoded by the cDNA or genomic DNA fragments.
Claim 11
Independent Claim
107 words
A method for identifying a protein activity of interest comprising: culturing a gene expression library, comprising a pool of expression constructs, each expression construct comprising a vector containing one or more cDNA or genomic DNA fragments, wherein the cDNA or genomic DNA fragments in the pool of expression constructs are derived from a plurality of species of donor microorganisms, and wherein the cDNA or genomic DNA fragments are each operably-associated with one or more regulatory regions that drives expression of genes encoded by the cDNA or genomic DNA fragments in an appropriate host organism; and detecting the protein activity encoded by the cDNA or genomic DNA fragments.
Claim 12
Dependent Claim
13 words
The method of claim 9, wherein the protein activity is an enzymatic activity.
Claim 13
Dependent Claim
24 words
The method of claim 10, wherein the enzymatic activity is selected from the group consisting of oxidoreductase, transferase, hydrolase, lyase, isomerase, and ligase activity.
Claim 14
Dependent Claim
14 words
The method of claim 9, wherein the microorganisms are derived from an environmental sample.
Claim 15
Dependent Claim
15 words
The method of claim 9, wherein the microorganisms are a mixed population of uncultured organisms.
Claim 16
Dependent Claim
17 words
The method of claim 9, wherein the DNA fragment comprises one or more operons, or portions thereof.
Claim 17
Dependent Claim
18 words
The method of claim 14, wherein the operon or portions thereof encodes a complete or partial metabolic pathway.
References Cited (Backward Citations)
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| 3.5 Years (Year 4) | 2002-09-27 | 2003-03-29 | 2003-09-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2006-09-27 | 2007-03-29 | 2007-09-28 | $3760.00 | Window Closed / Lapsed |
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