US5944754A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Tissue re-surfacing with hydrogel-cell compositions
Assignee: VBI Technologies LLC
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Filing: 1996-11-08
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Granted: 1999-08-31
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Expires: 2016-11-08
Abstract
Abstract The invention provides compositions and methods for generating new tissue on a surface, e.g., a surface of damaged or lost tissue, of a structure or organ in a mammal, The methods involve applying a thin layer of a liquid hydrogel-cell composition to the surface; and allowing the liquid hydrogel-cell composition to solidify, thereby forming a matrix that enables the tissue precursor cells to grow and generate new tissue. The surface can be internal, e.g., the surface of an organ or the internal surface of a blood vessel, or external, e.g., skin.
Inventors
- 👤 Charles A. Vacanti
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 20:29
CPC Classifications
A; A61; A61L; A61L27/00; A61L27/36; A61L27/38; A61L27/3839; A61L27/3843; A61K; A61K47/00; A61K47/06; A61K47/08; A61K47/10; A61K47/30; A61K47/36; A61L27/3804; A61L27/3813; A61L27/3817; A61L27/3847; A61L27/3852; A61L27/3886; A61L27/3891; A61L27/50; A61L27/52; C; C12; C12N; C12N5/00; C12N5/0068; C12N2533/00; C12N2533/30; C12N2533/70; C12N2533/74; C12N2539/00; C12N2539/10
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
81 words
A method for generataing new living tissue on and external surface of a tissue structure or organ in a mammal, said method comprising: a) obtaining a liquid hydrogel-cell composition comprising a hydrogel and live tissue precursor cells; b) applying a thin layer of said liquid hydrogel-cell composition to the external surface in the mammal; and c) allowing said applied liquid hydrogel-cell composition to solidify, thereby forming a matrix that enables the tissue precursor cells to grow and generate new living tissue.
Claim 2
Independent Claim
81 words
A method for generataing new living tissue on and external surface of a tissue structure or organ in a mammal, said method comprising: a) obtaining a liquid hydrogel-cell composition comprising a hydrogel and live tissue precursor cells; b) applying a thin layer of said liquid hydrogel-cell composition to the external surface in the mammal; and c) allowing said applied liquid hydrogel-cell composition to solidify, thereby forming a matrix that enables the tissue precursor cells to grow and generate new living tissue.
Claim 3
Dependent Claim
20 words
A method of claim 1, wherein said hydrogel-cell composition is solidified upon exposure to the body temperature of the mammal.
Claim 4
Dependent Claim
45 words
A method of claim 1, wherein said hydrogel-cell composition is solidified by interaction with ions selected from the group consisting of copper, calcium, aluminum, magnesium, strontium, barium, tin, and di-, tri- or tetra-functional organic cations, low molecular weight dicarboxylate ions, sulfate ions, and carbonate ions.
Claim 5
Dependent Claim
15 words
A method of claim 1, wherein said hydrogel-cell composition is solidified upon exposure to radiation.
Claim 6
Dependent Claim
43 words
A method of claim 1, wherein the hydrogel is selected from the group consisting of polysaccharides, proteins, polyphosphazenes, poly(oxyethylene)-poly(oxypropylene) block polymers, poly(oxyethylene)-poly(oxypropylene) block polymers of ethylene diamine, poly(acrylic acids), poly(methacrylic acids), copolymers of acrylic acid and methacrylic acid, poly(vinyl acetate), and sulfonated polymers.
Claim 7
Dependent Claim
17 words
A method of claim 1, wherein said hydrogel-cell composition is further stabilized by cross-linking with a polyion.
Claim 8
Dependent Claim
68 words
A method of claim 1, wherein the tissue precursor cells are selected from the group consisting of epidermal cells, chondrocytes and other cells that form cartilage, macrophages, dermal cells, muscle cells, hair follicles, fibroblasts, organ cells, osteoblasts and other cells that form bone, endothelial cells, mucosal cells, pleural cells, ear canal cells, tympanic membrane cells, peritoneal cells, Schwann cells, corneal epithelial cells, gingiva cells, and tracheal epithelial cells.
Claim 9
Dependent Claim
22 words
A method of claim 1, wherein said liquid hydrogel-cell composition is applied by painting, spraying, or dripping said composition onto the surface.
Claim 10
Dependent Claim
43 words
A method of claim 1, wherein said method further includes the use of a biocompatible amount of an adhesive selected from the group consisting of methyl alpha-cyanoacrylate, methacrylate, 2-cyano-2-propenoic acid methyl ester, methyl 2-cyanoacrylate, 2-cyanoacrylic acid methyl ester, and n-butyl cyanoacrylate based glue.
Claim 11
Dependent Claim
22 words
A method of claim 1, wherein said mammal is a human, mouse, rat, rabbit, cow, pig, horse, goat, sheep, dog, or cat.
Claim 12
Dependent Claim
25 words
The method of claim 1, wherein the applied thin layer has a thickness of from about tens of microns to between 0.5 and 5.0 mm.
Claim 13
Dependent Claim
14 words
The method of claim 1, wherein the matrix degrades as the new tissue grows.
Claim 14
Independent Claim
80 words
A method for generating new living skin on an external surface of damaged skin on a mammal, said method comprising: a) obtaining a liquid hydrogel-cell composition comprising a hydrogel and skin precursor cells; b) applying a thin layer of said liquid hydrogel-cell composition to the external surface of damaged skin on the mammal; and c) allowing said applied liquid hydrogel-cell composition to solidify, thereby forming a matrix that enables the skin precursor cells to grow and generate new living skin.
Claim 15
Independent Claim
80 words
A method for generating new living skin on an external surface of damaged skin on a mammal, said method comprising: a) obtaining a liquid hydrogel-cell composition comprising a hydrogel and skin precursor cells; b) applying a thin layer of said liquid hydrogel-cell composition to the external surface of damaged skin on the mammal; and c) allowing said applied liquid hydrogel-cell composition to solidify, thereby forming a matrix that enables the skin precursor cells to grow and generate new living skin.
Claim 16
Dependent Claim
30 words
A method of claim 13, wherein said hydrogel-cell composition is applied in two layers, with a first hydrogel-cell layer comprising dermal cells and a second hydrogel-cell layer comprising epidermal cells.
Claim 17
Independent Claim
78 words
A method for generating new living cartilage on an external surface of damaged cartilage in a mammal, said method comprising: a) obtaining a liquid hydrogel-cell composition comprising a hydrogel and live chondrocytes; b) applying a thin layer of said liquid hydrogel-cell composition to the external surface of the damaged cartilage in the mammal; and c) allowing said applied liquid hydrogel-cell composition to solidify, thereby forming a matrix that enables the chondrocytes to grow and generate new living cartilage.
Claim 18
Independent Claim
78 words
A method for generating new living cartilage on an external surface of damaged cartilage in a mammal, said method comprising: a) obtaining a liquid hydrogel-cell composition comprising a hydrogel and live chondrocytes; b) applying a thin layer of said liquid hydrogel-cell composition to the external surface of the damaged cartilage in the mammal; and c) allowing said applied liquid hydrogel-cell composition to solidify, thereby forming a matrix that enables the chondrocytes to grow and generate new living cartilage.
Claim 19
Dependent Claim
28 words
The method of claim 15, wherein said hydrogel is a temperature-sensitive hydrogel, and said liquid hydrogel-cell composition is solidified upon exposure to the body temperature of said mammal.
Claim 20
Dependent Claim
27 words
The method of claim 15, wherein said liquid hydrogel-cell composition is applied to said damaged cartilage surface by painting, spraying, or dripping said composition onto the surface.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2002-08-30 | 2003-03-01 | 2003-08-31 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2006-08-30 | 2007-03-01 | 2007-08-31 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2010-08-30 | 2011-03-01 | 2011-08-31 | $7700.00 | Window Closed / Lapsed |