US5916266A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Raw membranous material for medical materials and manufacturing methods thereof
Assignee: Bio Engineering Laboratories Ltd
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Filing: 1997-09-15
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Granted: 1999-06-29
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Expires: 2017-09-15
Abstract
Abstract A raw membrane material having an acellular nature and consisting substantially of a compact layer whose characteristic matrix structure is retained intact and which is produced by dissolving and removing cellular layers including epithelium and fibroblast layers from a biogenic, connective tissue membrane which comprises epithelial, basement membrane, compact and fibroblast layer.
Inventors
- 👤 Tooru Yui
- 👤 Tokuzo Nakagawa
- 👤 Kazuo Kondoh
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 06:09
CPC Classifications
A; A61; A61L; A61L27/00; A61L27/36; A61L27/3604; A61L15/00; A61L15/16; A61L15/40; A61L15/42; A61L15/64; A61L27/3683; A61L27/3687; A61L31/00; A61L31/005
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
135 words
A method for manufacturing a raw membrane material, comprising the steps of: (i) positively charging and denaturing biogenic connective tissue which comprises epithelium, basement membrane, compact and fibroblast layers with an aqueous solution of a quaternary ammonium salt of the formula: C.sub.6 H.sub.5 CH.sub.2 N(CH.sub.3).sub.2 R!.sup.+ Cl.sup.- wherein R is an alkyl group of 8 to 10 carbon atoms; (ii) treating the biogenic connective tissue at a pH of about 7 with thiolprotease which is a glycoprotein having a molecular weight of approximately 26,000 and an isoelectric point of 9; and (iii) thereafter, subjecting the biogenic connective tissue obtained from step (ii) to ultrasonic washing with water, thereby producing said raw membrane material having an acellular nature and consisting essentially of the compact layer of said biogenic connective tissue starting material employed in step (i).
Claim 2
Independent Claim
135 words
A method for manufacturing a raw membrane material, comprising the steps of: (i) positively charging and denaturing biogenic connective tissue which comprises epithelium, basement membrane, compact and fibroblast layers with an aqueous solution of a quaternary ammonium salt of the formula: C.sub.6 H.sub.5 CH.sub.2 N(CH.sub.3).sub.2 R!.sup.+ Cl.sup.- wherein R is an alkyl group of 8 to 10 carbon atoms; (ii) treating the biogenic connective tissue at a pH of about 7 with thiolprotease which is a glycoprotein having a molecular weight of approximately 26,000 and an isoelectric point of 9; and (iii) thereafter, subjecting the biogenic connective tissue obtained from step (ii) to ultrasonic washing with water, thereby producing said raw membrane material having an acellular nature and consisting essentially of the compact layer of said biogenic connective tissue starting material employed in step (i).
Claim 3
Dependent Claim
13 words
The method of claim 1, wherein the biogenic connective tissue is human amnion.
Claim 4
Dependent Claim
20 words
The method of claim 2, wherein the front and back faces of the connective tissue are asymmetric to each other.
Claim 5
Dependent Claim
10 words
The method of claim 1, wherein the thiolprotease is ficin.
Claim 6
Dependent Claim
17 words
The method of claim 4, wherein said ficin is combined with NaN 3 in a phosphate buffer.
Claim 7
Dependent Claim
69 words
A raw membrane material having an acellular nature and consisting essentially of a compact layer, having front and back faces, whose characteristic matrix structure is retained intact and having front and back faces, said compact layer being produced by dissolving and removing the cellular layers of the epithelial layer, the basement membrane, the fibroblast layer and the spongy layer, leaving the compact layer intact, from a biogenic connective tissue.
Claim 8
Dependent Claim
69 words
A raw membrane material having an acellular nature and consisting essentially of a compact layer, having front and back faces, whose characteristic matrix structure is retained intact and having front and back faces, said compact layer being produced by dissolving and removing the cellular layers of the epithelial layer, the basement membrane, the fibroblast layer and the spongy layer, leaving the compact layer intact, from a biogenic connective tissue.
Claim 9
Dependent Claim
41 words
The raw membrane material of claim 6, wherein said biogenic connective tissue is mater encephali, pericardium pleura, pelvic peritoneum, diaphragm, peritoneum, fascia, mesenterium, skin, tympanic membrane, or vascular, oesophageal, tracheal, urethral, ureteral or cardia wall material, fetal membrane, amnion or chorion.
Claim 10
Dependent Claim
15 words
The raw membrane material of claim 6, wherein the biogenic connective tissue is human amnion.
Claim 11
Dependent Claim
21 words
The raw membrane material of claim 8, wherein the membrane material is asymmetric with respect to said front and back faces.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2002-06-28 | 2002-12-28 | 2003-06-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2006-06-28 | 2006-12-28 | 2007-06-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2010-06-28 | 2010-12-28 | 2011-06-29 | $7700.00 | Window Closed / Lapsed |