US5902880A
Expired / Lapsed
US
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RNA polymerase III-based expression of therapeutic RNAs
Assignee: Sirna Therapeutics Inc
•
Filing: 1994-11-10
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Granted: 1999-05-11
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Expires: 2014-11-10
Abstract
Abstract A transcribed non-naturally occuring RNA molecule comprising a desired RNA molecule, wherein the 3' region of the RNA is able to base-pair with at least 8 bases at the 5' terminus of the same RNA molecule.
Inventors
- 👤 James Thompson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 16:33
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/11; C12N15/113; C12N15/1131; C07; C07H; C07H19/00; C07H19/02; C07H19/04; C07H19/06; C07H19/10; C07H19/16; C07H19/20; C07H21/00; C12N15/10; C12N15/1003; C12N15/1006; C12N15/101; C12N15/1136; C12N15/1137; C12N15/1138; C12Y; C12Y304/00; C12Y304/24; C12Y304/24017; A; A61; A61K; A61K38/00; A61K48/00; C12N2310/00; C12N2310/10; C12N2310/11; C12N2310/111; C12N2310/12; C12N2310/121; C12N2310/122; C12N2310/123; C12N2310/124; C12N2310/1241; C12N2310/126; C12N2310/127; C12N2310/30; C12N2310/31; C12N2310/315; C12N2310/32; C12N2310/321; C12N2310/322; C12N2310/33; C12N2310/332; C12N2310/333; C12N2310/334; C12N2310/335; C12N2310/336; C12N2310/34; C12N2310/346; C12N2310/35; C12N2310/351; C12N2310/3513; C12N2310/352; C12N2310/3523; C12N2310/3527; C12N2310/353; C12N2310/3533; C12N2310/3535
Claims Analysis
Total Claims: 29
Claim 1
Independent Claim
89 words
A non-naturally occurring RNA molecule having the formula: ##STR1## Wherein, N is independently a ribonucleotide which may be same or different, • indicates hydrogen bond formation between adjacent nucleotides, m is an image which is greater than or equal to 8; p is an integer which is greater then or equal to 1 if (N) p is present; q is an integer which is greater than or equal to 1 if (N) q is present, x is an integer which is 0 or 1; and is a desired RNA.
Claim 2
Dependent Claim
89 words
A non-naturally occurring RNA molecule having the formula: ##STR1## Wherein, N is independently a ribonucleotide which may be same or different, • indicates hydrogen bond formation between adjacent nucleotides, m is an image which is greater than or equal to 8; p is an integer which is greater then or equal to 1 if (N) p is present; q is an integer which is greater than or equal to 1 if (N) q is present, x is an integer which is 0 or 1; and is a desired RNA.
Claim 3
Dependent Claim
119 words
A non-naturally occurring RNA molecule having the formula: ##STR2## Wherein, N is independently a ribonucleotide which may be same or different, • indicates hydrogen bond formulation between adjacent nucleotides; m is an integer which is greater than or equal to 8; p is an integer which is greater than or equal to 1 if (N) p is present; q is an integer which is greater than or equal to 1 if (N) q is present; r is an integer which is greater than or equal to 1 if (N) r is present; s is an integer which is greater than or equal to 5; x is an integer which is 0 or 1; and D is a desired RNA.
Claim 4
Dependent Claim
119 words
A non-naturally occurring RNA molecule having the formula: ##STR2## Wherein, N is independently a ribonucleotide which may be same or different, • indicates hydrogen bond formulation between adjacent nucleotides; m is an integer which is greater than or equal to 8; p is an integer which is greater than or equal to 1 if (N) p is present; q is an integer which is greater than or equal to 1 if (N) q is present; r is an integer which is greater than or equal to 1 if (N) r is present; s is an integer which is greater than or equal to 5; x is an integer which is 0 or 1; and D is a desired RNA.
Claim 5
Dependent Claim
142 words
A non-naturally RNA molecule having the formula; ##STR3## Wherein, N is independently a ribonucleotide which may be same or different; • indicates hydrogen bond formation between adjacent nucleotides; A BOX represents RNA polymerase III type 2 consensus sequence; B BOX represents RNA polymerase III type 2 consensus sequence; m is an integer which is greater than or equal to 8; p is an integer which is greater than or equal to 1 if (N) p is present; q is an integer which is greater than or equal to 1 if (N) q is present; r is an integer which is greater than or equal to 1 if (N) r is present; s is an integer which is greater than or equal to 1 (N) s is present; x is an integer which is 0 or 1; an D is a desired RNA.
Claim 6
Dependent Claim
142 words
A non-naturally RNA molecule having the formula; ##STR3## Wherein, N is independently a ribonucleotide which may be same or different; • indicates hydrogen bond formation between adjacent nucleotides; A BOX represents RNA polymerase III type 2 consensus sequence; B BOX represents RNA polymerase III type 2 consensus sequence; m is an integer which is greater than or equal to 8; p is an integer which is greater than or equal to 1 if (N) p is present; q is an integer which is greater than or equal to 1 if (N) q is present; r is an integer which is greater than or equal to 1 if (N) r is present; s is an integer which is greater than or equal to 1 (N) s is present; x is an integer which is 0 or 1; an D is a desired RNA.
Claim 7
Dependent Claim
139 words
A non-naturally occurring RNA molecule having the formula: ##STR4## Wherein, N is independently a ribonucleotide which may be same or different; • indicates hydrogen bond formation between adjacent nucleotides; A BOX represents RNA polymerase III type 2 consensus sequence; B BOX represents RNA polymerase III type 2 consensus sequence; m is an integer which is greater than or equal to 8; p is an integer which is greater than or equal to 1 if (N) p is present; q is an integer which is greater than or equal to 1 if (N) q is present; r is an integer which is greater than or equal to 1 if (N) r is present s is an integer which is greater than or equal to 5; x is an integer which is 0 or 1; and D is a desired RNA.
Claim 8
Dependent Claim
139 words
A non-naturally occurring RNA molecule having the formula: ##STR4## Wherein, N is independently a ribonucleotide which may be same or different; • indicates hydrogen bond formation between adjacent nucleotides; A BOX represents RNA polymerase III type 2 consensus sequence; B BOX represents RNA polymerase III type 2 consensus sequence; m is an integer which is greater than or equal to 8; p is an integer which is greater than or equal to 1 if (N) p is present; q is an integer which is greater than or equal to 1 if (N) q is present; r is an integer which is greater than or equal to 1 if (N) r is present s is an integer which is greater than or equal to 5; x is an integer which is 0 or 1; and D is a desired RNA.
Claim 9
Dependent Claim
130 words
A non-naturally occuring RNA molecule having the formula: ##STR5## Wherein, N is independently a ribonucleotide which may be same or different; • indicates hydrogen bond formation between adjacent nucleotides; A BOX represents RNA polymerase III type 2 consensus sequence; B BOX represents RNA polymerase III type 2 consensus sequence; m is an integer which is greater than or equal to 8; p is an integer which is greater than or equal to 1 if (N) p is present; q is an integer which is greater than or equal to 1 if (N) q is present; r is an integer which is greater than or equal to 1 if (N) q is present; s is an integer which is greater than or equal to 5; and D is a desired RNA.
Claim 10
Dependent Claim
130 words
A non-naturally occuring RNA molecule having the formula: ##STR5## Wherein, N is independently a ribonucleotide which may be same or different; • indicates hydrogen bond formation between adjacent nucleotides; A BOX represents RNA polymerase III type 2 consensus sequence; B BOX represents RNA polymerase III type 2 consensus sequence; m is an integer which is greater than or equal to 8; p is an integer which is greater than or equal to 1 if (N) p is present; q is an integer which is greater than or equal to 1 if (N) q is present; r is an integer which is greater than or equal to 1 if (N) q is present; s is an integer which is greater than or equal to 5; and D is a desired RNA.
Claim 11
Dependent Claim
49 words
The RNA molecule of any of claims 3, 4 or 5, wherein said A BOX has the sequence 5'-URGCNNAGYGG-3', wherein R is a purine residue, N is a ribonucleotide, and Y is a pyrimidine residue; and wherein said B BOX has the sequence GGUUCGANUCC, wherein N is a ribonucleotide.
Claim 12
Dependent Claim
17 words
The RNA molecule of any of claims 1-5, wherein said desired RNA is an enzymatic RNA molecule.
Claim 13
Dependent Claim
16 words
The RNA molecule of claim 7, wherein said enzymatic RNA molecule is in a hammerhead configuration.
Claim 14
Dependent Claim
16 words
The RNA molecule of claim 7, wherein said enzymatic RNA molecule is in a hairpin configuration.
Claim 15
Dependent Claim
31 words
The RNA molecule of claim 7, wherein said enzymatic RNA molecule is in a group I ribozyme, group II ribozyme, VS ribozyme, hepatitis delta virus ribozyme or RNase P ribozyme configuration.
Claim 16
Dependent Claim
21 words
The RNA molecule of any of claims 1-5, wherein said molecule is transcribed by a RNA polymerase III based promoter system.
Claim 17
Dependent Claim
22 words
The RNA molecule of any of claims 1-5, wherein said molecule is transcribed by a type 2 RNA polymerase III promoter system.
Claim 18
Dependent Claim
15 words
The RNA molecule of any of claims 1-5, wherein, said molecule is a chimeric tRNA.
Claim 19
Dependent Claim
29 words
The RNA molecule of claim 12, wherein the A and the B boxes of said type 2 RNA polymerase III promoter, is separated by between 5 and 300 bases.
Claim 20
Dependent Claim
23 words
The RNA molecule of claim 12, wherein said desired RNA molecule includes the B box of said type 2 RNA polymerase III promoter.
Claim 21
Dependent Claim
34 words
The RNA molecule of any of claims 1-5, wherein said desired RNA molecule is selected from the group consisting of antisense RNA, decoy RNA, therapeutic editing RNA, enzymatic RNA, agonist RNA and antagonist RNA.
Claim 22
Dependent Claim
18 words
The RNA molecule of any of claims 1-5, wherein said m is greater than or equal to 12.
Claim 23
Dependent Claim
14 words
The RNA-molecule of claim 1, wherein m is greater than or equal to 15.
Claim 24
Dependent Claim
12 words
A DNA vector encoding the RNA molecule of any of claims 1-5.
Claim 25
Dependent Claim
12 words
A RNA vector encoding the RNA molecule of any of claims 1-5.
Claim 26
Dependent Claim
22 words
The vector of claim 19, wherein the portions of the vector encoding said RNA molecule function as a RNA pol III promoter.
Claim 27
Dependent Claim
8 words
A Cell comprising the vector of claim 19.
Claim 28
Dependent Claim
8 words
A Cell comprising the vector of claim 4.
Claim 29
Dependent Claim
10 words
A Cell comprising the RNA of any of claims 1-5.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2002-05-10 | 2002-11-09 | 2003-05-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2006-05-10 | 2006-11-09 | 2007-05-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2010-05-10 | 2010-11-09 | 2011-05-11 | $7700.00 | Window Closed / Lapsed |