US5882877A
Expired / Lapsed
US
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Adenoviral vectors for gene therapy containing deletions in the adenoviral genome
Assignee: Genzyme Corp
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Filing: 1997-07-16
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Granted: 1999-03-16
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Expires: 2017-07-16
Abstract
Abstract Adenoviral vectors which contain deletions of the early regions and/or late genes provide efficient delivery and expression of foreign nucleic acids of interest to patients. These vectors have a particular use in the treatment of cystic fibrosis patients. Furthermore, PAV vectors provide for a second generation of adenoviral vectors that contain the 5' ITR's, the packaging signal and the E1A enhancer. Other adenoviral vectors contain a deletion of the E1 region or a deletion of E4 but retain orf3 or orf6, and can either retain or delete the E3 region.
Inventors
- 👤 Richard J. Gregory
- 👤 Donna Armentano
- 👤 Larry A. Couture
- 👤 Alan E. Smith
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 20:54
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/85; C12N15/86; A; A61; A61K; A61K48/00; C07; C07K; C07K14/00; C07K14/435; C07K14/46; C07K14/47; C07K14/4701; C07K14/4712; A61K38/00; C12N2710/00; C12N2710/00011; C12N2710/10011; C12N2710/10311; C12N2710/10341; C12N2710/10343; C12N2830/00; C12N2830/42; C12N2840/00; C12N2840/20; C12N2840/203
Claims Analysis
Total Claims: 27
Claim 1
Independent Claim
40 words
An adenoviral vector comprising an adenovirus genome from which the E1, E2, E3 and E4 regions and late genes of the adenovirus genome have been deleted and additionally comprising a nucleic acid of interest operably linked to expression control sequences.
Claim 2
Dependent Claim
40 words
An adenoviral vector comprising an adenovirus genome from which the E1, E2, E3 and E4 regions and late genes of the adenovirus genome have been deleted and additionally comprising a nucleic acid of interest operably linked to expression control sequences.
Claim 3
Dependent Claim
29 words
The vector of claim 1 wherein the vector comprises the 5' and 3' inverted terminal repeat sequences, 5' packaging sequences and the E1A enhancer sequences of the adenovirus genome.
Claim 4
Dependent Claim
18 words
The vector of claim 2 wherein the vector further comprises a nuclear localization signal and a polyadenylation signal.
Claim 5
Dependent Claim
14 words
The vector of claim 3 wherein the expression control sequences comprise a PGK promoter.
Claim 6
Dependent Claim
41 words
The vector of claim 3 wherein the expression control sequences comprise the adenovirus major late promoter and the adenovirus tripartite leader, the nuclear localization signal comprises the SV40 T-antigen nuclear localization signal and the polyadenylation signal comprises the SV40 polyadenylation signal.
Claim 7
Dependent Claim
15 words
The vector of claim 5 wherein the vector further comprises an internal ribosomal entry site.
Claim 8
Dependent Claim
17 words
The vector of claim 6 wherein the internal initiation signal is the EMC internal ribosomal entry signal.
Claim 9
Dependent Claim
42 words
An adenoviral vector comprising the 5' and 3' inverted terminal repeat (ITR) sequences, the 5' packaging sequences and the E1A enhancer sequences of the adenovirus genome, wherein the vector can accommodate a foreign nucleic acid of up to 36 kb in length.
Claim 10
Dependent Claim
42 words
An adenoviral vector comprising the 5' and 3' inverted terminal repeat (ITR) sequences, the 5' packaging sequences and the E1A enhancer sequences of the adenovirus genome, wherein the vector can accommodate a foreign nucleic acid of up to 36 kb in length.
Claim 11
Dependent Claim
19 words
The vector of claim 8 further comprising a foreign nucleic acid of interest operably linked to expression control sequences.
Claim 12
Dependent Claim
28 words
The vector of claim 8 wherein the 5' inverted terminal repeat sequences, the 5' packaging sequences and the E1A enhancer sequence comprise nucleotides 0-358 of the adenoviral genome.
Claim 13
Dependent Claim
28 words
The vector of claim 8 wherein the 5' inverted terminal repeat sequences, the 5' packaging sequences and the E1A enhancer sequence comprise nucleotides 0-380 of the adenoviral genome.
Claim 14
Dependent Claim
4 words
A PAV I vector.
Claim 15
Dependent Claim
4 words
A PAV I vector.
Claim 16
Dependent Claim
4 words
A PAV II vector.
Claim 17
Dependent Claim
4 words
A PAV II vector.
Claim 18
Independent Claim
78 words
A method for producing an adenoviral vector comprising an adenovirus genome from which the E1, E2, E3 and E4 regions and late genes of the adenovirus genome have been deleted and additionally comprising a foreign nucleic acid of interest operably linked to expression control sequences, the method comprising introducing the adenoviral vector nucleic acid and a helper virus nucleic acid into an adenovirus producer cell line and purifying the adenoviral vector and helper virus from said cell line.
Claim 19
Independent Claim
78 words
A method for producing an adenoviral vector comprising an adenovirus genome from which the E1, E2, E3 and E4 regions and late genes of the adenovirus genome have been deleted and additionally comprising a foreign nucleic acid of interest operably linked to expression control sequences, the method comprising introducing the adenoviral vector nucleic acid and a helper virus nucleic acid into an adenovirus producer cell line and purifying the adenoviral vector and helper virus from said cell line.
Claim 20
Independent Claim
95 words
A method for producing an adenoviral vector comprising the 5' and 3' inverted terminal repeat (ITR) sequences, the 5' packaging sequences and the E1A enhancer sequences of the adenoviral genome, wherein the vector can accommodate a foreign nucleic acid of up to 36 kb in length, the vector additionally comprising a foreign nucleic acid of interest operably linked to expression control sequences, the method comprising introducing the adenoviral vector nucleic acid and a helper virus nucleic acid into an adenovirus producer cell line and purifying the adenoviral vector and helper virus from said cell line.
Claim 21
Independent Claim
95 words
A method for producing an adenoviral vector comprising the 5' and 3' inverted terminal repeat (ITR) sequences, the 5' packaging sequences and the E1A enhancer sequences of the adenoviral genome, wherein the vector can accommodate a foreign nucleic acid of up to 36 kb in length, the vector additionally comprising a foreign nucleic acid of interest operably linked to expression control sequences, the method comprising introducing the adenoviral vector nucleic acid and a helper virus nucleic acid into an adenovirus producer cell line and purifying the adenoviral vector and helper virus from said cell line.
Claim 22
Dependent Claim
18 words
The method of claim 14 or 15, further comprising purifying the adenoviral vector by cesium chloride gradient centrifugation.
Claim 23
Dependent Claim
32 words
The method of claim 14 or 15, wherein the helper virus supplies adenovirus replication and/or adenoviral structural proteins to the adenoviral vector, thereby allowing said adenoviral vector to be replicated and packaged.
Claim 24
Dependent Claim
16 words
The method of claim 17, wherein the helper virus is a packaging and/or replication deficient adenovirus.
Claim 25
Dependent Claim
14 words
The method of claim 17, wherein the helper virus is a packaging defective adenovirus.
Claim 26
Dependent Claim
16 words
The method of claim 19 wherein the helper virus has mutations in the adenovirus packaging sequences.
Claim 27
Dependent Claim
14 words
The method of claim 17, wherein the helper virus is a replication deficient adenovirus.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2002-03-15 | 2002-09-14 | 2003-03-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2006-03-15 | 2006-09-14 | 2007-03-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2010-03-15 | 2010-09-14 | 2011-03-16 | $7700.00 | Window Closed / Lapsed |