Abstract The present invention provides a polynucleotide (snpg) initially isolated from a colon cDNA library which identifies and encodes a novel human synaptogyrin homolog (SNPG). The invention provides for genetically engineered expression vectors and host cells comprising the nucleic acid sequence encoding SNPG. The invention also provides for the use of SNPG in the membrane of a vesicle containing a chemical or biological therapeutic composition for the treatment of disease, and for the therapeutic use of antisense molecules, antibodies, antagonists or inhibitors in the treatment of conditions or diseases associated with the abnormal or excess expression of SNPG. The invention also describes diagnostic assays which utilize diagnostic compositions comprising the polynucleotide, fragments or the complement thereof, which hybridize with the genomic sequence or the transcript of snpg, or anti-SNPG antibodies which specifically bind to the polypeptide, SNPG.
A purified polynucleotide encoding a polypeptide with an amino acid sequence shown in SEQ ID NO:2.
Claim 2
Dependent Claim
16 words
A purified polynucleotide encoding a polypeptide with an amino acid sequence shown in SEQ ID NO:2.
Claim 3
Dependent Claim
18 words
The polynucleotide of claim 1 having the nucleic acid sequence consisting of SEQ ID NO:1, or its complement.
Claim 4
Dependent Claim
45 words
A method for detecting the polynucleotide of claim 1 in a biological sample, the method comprising the steps of: a) combining the biological sample with the polynucleotide of claim 1, under conditions suitable for the formation of hybridization complex; and b) detecting the hybridization complex.
Claim 5
Dependent Claim
9 words
An expression vector comprising the polynucleotide of claim 2.
Claim 6
Dependent Claim
11 words
A host cell transformed with the expression vector of claim 4.
Claim 7
Dependent Claim
49 words
A method for producing a polypeptide having the amino acid sequence consisting of SEQ ID NO:2, the method comprising the steps of: a) culturing the host cell of claim 5 under conditions suitable for the expression of the polypeptide; and b) recovering the polypeptide from the host cell culture.