US5837144A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of magnetically separating liquid components
Assignee: Roche Diagnostics GmbH
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Filing: 1995-06-15
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Granted: 1998-11-17
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Expires: 2015-06-15
Abstract
Abstract Method of separating a component of a liquid from other components by immobilizing the component to suspended magnetic particles in a vessel, immersing a magnetic device into the vessel while the device is separated from the liquid by means of a protective sleeve made of a non-magnetic material. Then the non-immobilized components are removed. The invention also addresses a device suitable for implementing this method. With said method and device, resuspension is achieved in a simple and efficient manner.
Inventors
- 👤 Gerhard Bienhaus
- 👤 Burkhard Stolz
- 👤 Ulrich Schubert
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 05:24
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54313; G01N33/54326; B; B03; B03C; B03C1/00; B03C1/02; B03C1/28; B03C1/284
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
166 words
A method of separating a first component of a liquid from other components in the liquid utilizing magnetic particles, comprising the steps of: (a) providing a vessel; (b) adding the liquid and a quantity of magnetic particles therein to form a suspension of the magnetic particles and the liquid within said vessel; (c) immobilizing the first component on the suspended magnetic particles in the vessel; (d) immersing a magnetic device into the suspension contained in the vessel, while separating the device from the suspension by means of a protective sleeve which is made of a non-magnetic material, to apply a magnetic field to the suspension to adhere the magnetic particles having the first component immobilized thereon to the protective sleeve, and thereafter (e) separating the other components from the immobilized first component by removing the liquid containing the other components from the vessel while maintaining the protective sleeve with the magnetic particles having the first component immobilized thereon adhering to the protective sleeve within the vessel.
Claim 2
Independent Claim
166 words
A method of separating a first component of a liquid from other components in the liquid utilizing magnetic particles, comprising the steps of: (a) providing a vessel; (b) adding the liquid and a quantity of magnetic particles therein to form a suspension of the magnetic particles and the liquid within said vessel; (c) immobilizing the first component on the suspended magnetic particles in the vessel; (d) immersing a magnetic device into the suspension contained in the vessel, while separating the device from the suspension by means of a protective sleeve which is made of a non-magnetic material, to apply a magnetic field to the suspension to adhere the magnetic particles having the first component immobilized thereon to the protective sleeve, and thereafter (e) separating the other components from the immobilized first component by removing the liquid containing the other components from the vessel while maintaining the protective sleeve with the magnetic particles having the first component immobilized thereon adhering to the protective sleeve within the vessel.
Claim 3
Dependent Claim
28 words
Method of claim 1, wherein in step (e) the liquid containing the non-immobilized components is removed from the vessel through bores in a bottom wall of the vessel.
Claim 4
Dependent Claim
38 words
Method of claim 1, further comprising the step of withdrawing the protective sleeve from the vessel with the magnetic particles adhered to the protective sleeve and with said first component immobilized on the magnetic particles after step (e).
Claim 5
Dependent Claim
41 words
Method of claim 2, wherein the magnetic device includes a longitudinal axis and a plurality of permanent magnets or electromagnets stacked along said axis, wherein said magnets define magnetic poles which alternate in polarity in a direction parallel to said axis.
Claim 6
Dependent Claim
10 words
Method of claim 1, wherein the first component is cells.
Claim 7
Dependent Claim
11 words
Method of claim 1, wherein the first component is nucleic acids.
Claim 8
Dependent Claim
43 words
Method of claim 1, further comprising the step of adding a wash liquid to the vessel after step (e), and re-suspending the magnetic particles with the first component immobilized thereon in said wash liquid to wash said magnetic particles and said first component.
Claim 9
Dependent Claim
24 words
Method of claim 7, wherein said step of re-suspending includes the step of rotating and/or lifting the magnetic device with respect to said vessel.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2001-11-16 | 2002-05-18 | 2002-11-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2005-11-16 | 2006-05-18 | 2006-11-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2009-11-16 | 2010-05-18 | 2010-11-17 | $7700.00 | Window Closed / Lapsed |