US5830696A
Expired / Lapsed
US
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Directed evolution of thermophilic enzymes
Assignee: BP Corp North America Inc
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Filing: 1996-12-05
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Granted: 1998-11-03
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Expires: 2016-12-05
Abstract
Abstract Thermostable enzyme are subjected to mutagenesis to produce a thermophilic enzyme which is stable at thermophilic temperature and which has increased activities at least two-fold higher than the activity of the wild-type thermostable enzyme at lower temperatures, which are temperatures of 50 DEG C. or lower.
Inventors
- 👤 Jay M. Short
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 23:58
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/10; C12N15/1034; C12N15/1058; C12N15/102; C12N9/00; C12N9/14; C12N9/16; C12N9/24; C12N9/2402; C12N9/2405; C12N9/2468; C12N9/2471; C12Y; C12Y302/00; C12Y302/01; C12Y302/01021; C12Y302/01023
Claims Analysis
Total Claims: 3
Claim 1
Independent Claim
113 words
A method for providing a thermostable enzyme having improved enzyme activities as compared to a corresponding wild-type enzyme at lower temperatures, comprising: (a) subjecting at least one polynucleotide encoding an enzyme which is stable at a temperature of at least 60° C. to a random mutagenesis; and (b) screening mutant enzymes produced in (a) for a mutated enzyme or for a polynucleotide encoding a mutated enzyme, wherein the mutated enzyme is stable at a temperature of at least 60° C.; has an enzyme activity at a temperature at least 10° C. below its optimal temperature range; and has activity greater than the enzyme encoded by the polynucleotide of step (a) prior to mutagenesis.
Claim 2
Independent Claim
113 words
A method for providing a thermostable enzyme having improved enzyme activities as compared to a corresponding wild-type enzyme at lower temperatures, comprising: (a) subjecting at least one polynucleotide encoding an enzyme which is stable at a temperature of at least 60° C. to a random mutagenesis; and (b) screening mutant enzymes produced in (a) for a mutated enzyme or for a polynucleotide encoding a mutated enzyme, wherein the mutated enzyme is stable at a temperature of at least 60° C.; has an enzyme activity at a temperature at least 10° C. below its optimal temperature range; and has activity greater than the enzyme encoded by the polynucleotide of step (a) prior to mutagenesis.
Claim 3
Dependent Claim
40 words
The method of claim 1, wherein the enzyme is selected from the group consisting of lipase, esterase, protease, glycosidase, glycosyl transferase, phosphatase, kinase, monoxygenase, dioxygenase, haloperoxidase, lignin peroxidase, diarylpropane peroxidase, epoxide hydrolase, nitrite hydratase, nitrile nitrilase, transaminase, amidase and acylase.
References Cited (Backward Citations)
US5352778A
Cited
US5500363A
Cited
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