US5808012A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Protein-enriched thermoplastics
Assignee: Protein Polymer Technologies Inc
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Filing: 1996-01-16
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Granted: 1998-09-15
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Expires: 2016-01-16
Abstract
Abstract Thermoplastics interdispersed with a variety of functional thermostable proteins and methods for their production are provided. To prepare the subject thermoplastics, a plastic material is contacted with a thermostable polypeptide and then subjected to the heating and molding/extrusion/casting process. The resultant thermoplastics comprise the thermostable polypeptide on the formed plastic surface and at a depth below the plastic surface. The thermostable polypeptides contained in the disclosed compositions retain functional properties or binding specificities through the heating and molding/extrusion/casting processes. Preferred thermostable polypeptides used in the disclosed compositions include silk-like protein polymers, particularly ProNectin TM F. The disclosed methods and compositions find use in many applications where plastics containing finctional thermostable proteins are desired, in particular, cell cultureware.
Inventors
- 👤 David M. Donofrio
- 👤 Erwin R. Stedronsky
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 05:37
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/0068; A; A61; A61L; A61L17/00; A61L29/00; A61L29/04; A61L29/049; C07; C07K; C07K14/00; C07K14/435; C07K14/43504; C07K14/43563; C07K14/43586; C07K14/78; C07K17/00; C07K17/02; C07K17/08; C12N1/00; C12N1/06; C12N15/00; C12N15/09; C12N15/11; C12N15/62; C12N15/63; C12N15/66; C12N15/70; C12N15/74; C12N15/75; C07K2319/00; C07K2319/01; C07K2319/02; C07K2319/33; C07K2319/61; C12N2533/00; C12N2533/30; C12N2533/50; C12N2533/52; Y; Y10; Y10S; Y10S530/00; Y10S530/81; Y10S530/812; Y10S530/815; Y10S530/817
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
67 words
A composition comprising a functional thermostable polypeptide interdispersed within a thermoplastic resulting from a melt at a temperature of at least 60° C., wherein said polypeptide comprises repeating units from elastin, collagen, keratin or silk proteins, and a sequence intervening said repeating units, wherein said intervening sequence has at least one chemically active amino acid or a naturally occurring sequence having binding specificity for a protein receptor.
Claim 2
Dependent Claim
67 words
A composition comprising a functional thermostable polypeptide interdispersed within a thermoplastic resulting from a melt at a temperature of at least 60° C., wherein said polypeptide comprises repeating units from elastin, collagen, keratin or silk proteins, and a sequence intervening said repeating units, wherein said intervening sequence has at least one chemically active amino acid or a naturally occurring sequence having binding specificity for a protein receptor.
Claim 3
Dependent Claim
18 words
The composition of claim 1 wherein said polypeptide is from 3 to about 60 amino acids in length.
Claim 4
Dependent Claim
16 words
The composition of claim 1 wherein said polypeptide has a molecular weight of at least 25kD.
Claim 5
Dependent Claim
11 words
The composition of claim 1 wherein said intervening sequence comprises RGD.
Claim 6
Dependent Claim
14 words
The composition of claim 1 wherein said protein polymer is ProNectin®F (SLPF) or SLPL3.0.
Claim 7
Dependent Claim
14 words
The composition of claim 1 wherein said thermoplastic is polystyrene, polyethylene, polypropylene, or polymethylmethacrylate.
Claim 8
Dependent Claim
12 words
The composition of claim 1 further comprising a polypeptide thermostability enhancing additive.
Claim 9
Dependent Claim
89 words
A formed object produced by contacting a thermoplastic with a functional thermostable polypeptide, heating said thermoplastic and said polypeptide to at least 60° C. for at least 15 seconds to create a melt, molding said melt to form a functional thermostable polypeptide interdispersed within said thermoplastic, wherein said polypeptide comprises repeating units from elastin, collagen, keratin or silk proteins, and a sequence intervening said repeating units, said intervening sequence having at least one chemically active amino acid or a naturally occurring sequence having binding specificity for a protein receptor.
Claim 10
Dependent Claim
89 words
A formed object produced by contacting a thermoplastic with a functional thermostable polypeptide, heating said thermoplastic and said polypeptide to at least 60° C. for at least 15 seconds to create a melt, molding said melt to form a functional thermostable polypeptide interdispersed within said thermoplastic, wherein said polypeptide comprises repeating units from elastin, collagen, keratin or silk proteins, and a sequence intervening said repeating units, said intervening sequence having at least one chemically active amino acid or a naturally occurring sequence having binding specificity for a protein receptor.
Claim 11
Independent Claim
95 words
A method for producing a functional thermostable polypeptide interdispersed within a thermoplastic, said method comprising: forming a mixture of thermoplastic and said functional thermostable polypeptide, heating said thermoplastic and said polypeptide to create a melt, molding said melt into a predetermined form, whereby a functional thermostable polypeptide is interdispersed within said thermoplastic, wherein said polypeptide comprises repeating units from elastin, collagen, keratin or silk proteins, and a sequence intervening said repeating units, said intervening sequence having at least one chemically active amino acid or a naturally occurring sequence having binding specificity for a protein receptor.
Claim 12
Independent Claim
95 words
A method for producing a functional thermostable polypeptide interdispersed within a thermoplastic, said method comprising: forming a mixture of thermoplastic and said functional thermostable polypeptide, heating said thermoplastic and said polypeptide to create a melt, molding said melt into a predetermined form, whereby a functional thermostable polypeptide is interdispersed within said thermoplastic, wherein said polypeptide comprises repeating units from elastin, collagen, keratin or silk proteins, and a sequence intervening said repeating units, said intervening sequence having at least one chemically active amino acid or a naturally occurring sequence having binding specificity for a protein receptor.
Claim 13
Independent Claim
51 words
A method for producing functional ProNectin®F (SLPF) interdispersed within a thermoplastic, said method comprising: forming a mixture of a thermoplastic and functional ProNectin®F (SLPF), heating said thermoplastic and said ProNectin®F (SLPF) to create a melt, molding said melt into a predetermined form, whereby functional ProNectin®F (SLPF) is interdispersed within said thermoplastic.
Claim 14
Independent Claim
51 words
A method for producing functional ProNectin®F (SLPF) interdispersed within a thermoplastic, said method comprising: forming a mixture of a thermoplastic and functional ProNectin®F (SLPF), heating said thermoplastic and said ProNectin®F (SLPF) to create a melt, molding said melt into a predetermined form, whereby functional ProNectin®F (SLPF) is interdispersed within said thermoplastic.
References Cited (Backward Citations)
JPS57108010A
Cited
US4617271A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2001-09-14 | 2002-03-16 | 2002-09-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2005-09-14 | 2006-03-16 | 2006-09-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2009-09-14 | 2010-03-16 | 2010-09-15 | $7700.00 | Window Closed / Lapsed |