Sets of labeled energy transfer fluorescent primers and their use in multi component analysis

Claim 2

A set of at least 2 different fluorescent labels, said set comprising: a first energy transfer fluorescent label comprising at least one donor fluorophore and at least one acceptor fluorophore in energy transfer relationship separated by a distance x; and a second energy transfer fluorescent label comprising said at least one donor fluorophore and said at least one acceptor fluorophore in energy transfer relationship separated by a distance y; wherein: (a) said donor fluorophores of said first and second labels are the same; (b) said acceptor fluorophores of said first and second labels are the same; and (c) x and y are sufficiently different so that said first and second fluorescent labels provide distinguishable fluorescent signals wherein all of the labels of said set are acceptor fluorophores are selected from the group consisting of TAM, JOE, FAM, ROX, BODIPY, fluoresein and cyanine dyes.

Claim 3

The set according to claim 1, wherein said set further comprises at least one non-energy transfer fluorescent label.

Claim 4

The set according to claim 1, wherein said set comprises n distinct energy transfer labels, wherein the total number of different acceptor fluorophores among said n distinct energy transfer labels does not exceed n-1.

Claim 5

The set according to claim 1, wherein said donor and acceptor fluorophores of said energy transfer labels are bonded to a polynucleotide.

Claim 6

The set according to claim 1, wherein said donor and acceptor fluorophores of said energy transfer labels are bonded to a spacer selected from the group consisting of a peptide nucleic acid and a peptide.

Claim 7

The set according to claim 1, wherein said energy transfer labels comprise an energy transfer cassette.

Claim 8

The set according to claim 1, wherein all of the labels of said set are excited at a common wavelength and said acceptor fluorophores are selected from the group consisting of TAM, JOE, FAM, BODIPY, fluorescein and cyanine dyes.

Claim 9

The set according to claim 3, wherein n is 4.

Claim 10

The set according to claim 8, wherein the number of different acceptor fluorophores among said 4 energy transfer labels is 2.

Claim 11

A set of four different fluorescently labeled energy transfer oligonucleotide primers, said set comprising: a first energy transfer fluorescently labeled oligonucleotide primer comprising a donor fluorophore and an acceptor fluorophore in energy transfer relationship separated by a distance x; a second energy transfer fluorescently labeled oligonucleotide primer comprising said donor fluorophore and said acceptor fluorophore in energy transfer relationship separated by a distance y; a third energy transfer fluorescently labeled oligonucleotide primer comprising said donor fluorophore and said acceptor fluorophore in energy transfer relationship separated by a distance z; and a fourth energy transfer fluorescently labeled oligonucleotide primer wherein said donor and acceptor fluorophores have the same fluorescence emission; wherein: (a) said donor fluorophore of said first, second and third labels are the same; (b) said acceptor fluorophores of said first, second and third labels are the same and selected from the group consisting of TAM, JOE, FAM, ROX, BODIPY, fluorescein and cyanine dyes; and (c) x, y and z are sufficiently different so that said first through fourth fluorescently labeled oligonucleotide primers provide distinguishable fluorescence emission patterns.

Claim 12

A set of four different fluorescently labeled energy transfer oligonucleotide primers, said set comprising: a first energy transfer fluorescently labeled oligonucleotide primer comprising a donor fluorophore and an acceptor fluorophore in energy transfer relationship separated by a distance x; a second energy transfer fluorescently labeled oligonucleotide primer comprising said donor fluorophore and said acceptor fluorophore in energy transfer relationship separated by a distance y; a third energy transfer fluorescently labeled oligonucleotide primer comprising said donor fluorophore and said acceptor fluorophore in energy transfer relationship separated by a distance z; and a fourth energy transfer fluorescently labeled oligonucleotide primer wherein said donor and acceptor fluorophores have the same fluorescence emission; wherein: (a) said donor fluorophore of said first, second and third labels are the same; (b) said acceptor fluorophores of said first, second and third labels are the same and selected from the group consisting of TAM, JOE, FAM, ROX, BODIPY, fluorescein and cyanine dyes; and (c) x, y and z are sufficiently different so that said first through fourth fluorescently labeled oligonucleotide primers provide distinguishable fluorescence emission patterns.

Claim 13

The set according to claim 10, wherein said first, second, third and fourth energy transfer labeled primers comprise a common donor fluorophore.

Claim 14

The set according to claim 10, wherein said energy transfer fluorescently labeled primers further comprise energy transfer cassettes.

Claim 15

The set according to claim 12, wherein said donor and acceptor fluorophores are bonded to a polysugar phosphate.

Claim 16

A set of four different energy transfer fluorescently labeled oligonucleotide primers for use in enzymatic sequencing, said set comprising: a first energy transfer fluorescently labeled oligonucleotide comprising a donor fluorophore bonded to the 5' terminus and an acceptor fluorophore bonded to the 7th nucleotide 3' from the 5' terminus; a second energy transfer fluorescently labeled oligonucleotide comprising said donor fluorophore bonded to the 5' terminus and said acceptor fluorophore bonded to the 14th nucleotide 3' from the 5' terminus; a third energy transfer fluorescently labeled oligonucleotide comprising said donor fluorophore bonded to the 5' terminus and said acceptor fluorophore bonded to the 17th nucleotide 3' from the 5' terminus; and a fourth energy transfer fluorescently labeled oligonucleotide comprising said donor fluorophore bonded to the 5' terminus and said donor fluorophore bonded to the 17th nucleotide 3' from the 5' terminus; wherein (a) said donor fluorophore of said first, second, third and fourth labeled oligonucleotides are the same; and (b) said acceptor fluorophores of said first, second, third and fourth labeled oligonucleotides are the same and are selected from the group consisting of TAM, JOE, FAM, ROX, BODIPY, fluorescein and cyanine dyes.

Claim 17

A set of four different energy transfer fluorescently labeled oligonucleotide primers for use in enzymatic sequencing, said set comprising: a first energy transfer fluorescently labeled oligonucleotide comprising a donor fluorophore bonded to the 5' terminus and an acceptor fluorophore bonded to the 7th nucleotide 3' from the 5' terminus; a second energy transfer fluorescently labeled oligonucleotide comprising said donor fluorophore bonded to the 5' terminus and said acceptor fluorophore bonded to the 14th nucleotide 3' from the 5' terminus; a third energy transfer fluorescently labeled oligonucleotide comprising said donor fluorophore bonded to the 5' terminus and said acceptor fluorophore bonded to the 17th nucleotide 3' from the 5' terminus; and a fourth energy transfer fluorescently labeled oligonucleotide comprising said donor fluorophore bonded to the 5' terminus and said donor fluorophore bonded to the 17th nucleotide 3' from the 5' terminus; wherein (a) said donor fluorophore of said first, second, third and fourth labeled oligonucleotides are the same; and (b) said acceptor fluorophores of said first, second, third and fourth labeled oligonucleotides are the same and are selected from the group consisting of TAM, JOE, FAM, ROX, BODIPY, fluorescein and cyanine dyes.

Claim 18

The set according to claim 14, wherein said donor fluorophore is FAM.

Claim 19

The set according to claim 14, wherein said acceptor fluorophore is ROX.

Claim 20

A kit for use in DNA sequencing, said kit comprising: a set of four fluorescently labeled oligonucleotide primers, wherein at least two of said oligonucleotide primers have a common donor and acceptor fluorophore in energy transfer relationship and are separated by different distances wherein all of the labels of said set are acceptor fluorophores are selected from the group consisting of TAM, JOE, FAM, ROX, BODIPY, fluoresein and cyanine dyes.

Claim 21

A kit for use in DNA sequencing, said kit comprising: a set of four fluorescently labeled oligonucleotide primers, wherein at least two of said oligonucleotide primers have a common donor and acceptor fluorophore in energy transfer relationship and are separated by different distances wherein all of the labels of said set are acceptor fluorophores are selected from the group consisting of TAM, JOE, FAM, ROX, BODIPY, fluoresein and cyanine dyes.

Claim 22

The kit according to claim 17, wherein said kit further comprises polymerase.

Claim 23

The kit according to claim 17, wherein said kit further comprises deoxynucleotides and dideoxynucleotides.

Claim 24

The kit according to claim 17, wherein said common acceptor fluorophore of said at least two oligonucleotide primers is selected from the group consisting of TAM, JOE, FAM, ROX, BODIPY, fluorescein and cyanine dyes.