US5798250A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Facilitating hepatitus virus infection in cell culture using a membrane stressor
Assignee: Bausch Health Americas Inc
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Filing: 1996-04-15
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Granted: 1998-08-25
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Expires: 2016-04-15
Abstract
Abstract Methods and compositions are provided for infecting cells with virus. Specific embodiments involved stressing the cellular membranes with chemical, electrical or mechanical means, such as by incubating the cells in DOTAP or Transfectam™. Other embodiments involve impregnating carriers with virus for use in research a suitable carrier such as a polymer or nanoparticle.
Inventors
- 👤 Fusun Zeytinoglu
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 23:01
CPC Classifications
C; C12; C12N; C12N7/00; C12N2770/00; C12N2770/00011; C12N2770/24011; C12N2770/24211; C12N2770/24251
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
50 words
A method of infecting cells with Hepatitis C virus comprising: providing cells in a culture medium; exposing the cells to the virus; and artificially exposing the cells to a sufficient amount of membrane stressor comprising at least one of DOTAP (N- 1-(2,3-Dioeoylocy)propyl!-N,N,N-trimethylammonium methylsulfate and Transfectam™ (dioctadecylamidoglcyl spermine) to facilitate infection.
Claim 2
Independent Claim
50 words
A method of infecting cells with Hepatitis C virus comprising: providing cells in a culture medium; exposing the cells to the virus; and artificially exposing the cells to a sufficient amount of membrane stressor comprising at least one of DOTAP (N- 1-(2,3-Dioeoylocy)propyl!-N,N,N-trimethylammonium methylsulfate and Transfectam™ (dioctadecylamidoglcyl spermine) to facilitate infection.
Claim 3
Dependent Claim
29 words
The method of claim 1 wherein facilitation of infection occurs when the percent of infected cells is at least one order of magnitude over that achieved without the stressor.
Claim 4
Dependent Claim
26 words
The method of claim 1 wherein facilitation of infection occurs when the percent of infected cells is at least ten times that achieved without the stressor.
Claim 5
Independent Claim
56 words
A method of infecting cells with Hepatitis virus comprising: providing cells in a culture medium; exposing the cells to the virus; and artificially exposing the cells to a sufficient amount of a membrane stressor wherein the membrane stressor is selected from at least one of DOTAP (N- 1-(2,3-Dioeoylocy)propyl!-N,N,N-trimethylammonium methylsulfate) and Transfectam™ (dioctadecylamidoglcyl spermine) to facilitate infection.
Claim 6
Independent Claim
56 words
A method of infecting cells with Hepatitis virus comprising: providing cells in a culture medium; exposing the cells to the virus; and artificially exposing the cells to a sufficient amount of a membrane stressor wherein the membrane stressor is selected from at least one of DOTAP (N- 1-(2,3-Dioeoylocy)propyl!-N,N,N-trimethylammonium methylsulfate) and Transfectam™ (dioctadecylamidoglcyl spermine) to facilitate infection.
References Cited (Backward Citations)
US5264618A
Cited
US5578475A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2001-08-24 | 2002-02-23 | 2002-08-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2005-08-24 | 2006-02-23 | 2006-08-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2009-08-24 | 2010-02-23 | 2010-08-25 | $7700.00 | Window Closed / Lapsed |