US5789251A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Multi-well bioassay tray with evaporation protection and method of use
Assignee: Individual
•
Filing: 1994-06-16
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Granted: 1998-08-04
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Expires: 2014-06-16
Abstract
Abstract A biopsy apparatus, including: a microplate having defined therein a plurality of vertical wells; and a layer of film disposed over the vertical wells to prevent the evaporation of liquid therefrom, the layer of film being penetrable by a pipette tip to access one of the vertical wells to add liquid to or withdraw liquid from the vertical well, the layer of film being resealable to reseal the area of penetration of the pipette tip when the pipette tip is withdrawn, in order to prevent evaporation of liquid from the vertical well.
Inventors
- 👤 Thomas W. Astle
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 07:55
CPC Classifications
B; B01; B01L; B01L3/00; B01L3/50; B01L3/508; B01L3/5085; B01L3/5082; B01L3/50825; G; G01; G01N; G01N35/00; G01N35/10; G01N35/1079; Y; Y10; Y10T; Y10T436/00; Y10T436/11; Y10T436/113332; Y10T436/114165; Y10T436/25; Y10T436/2575
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
221 words
A bioassay method, comprising: (a) providing a microplate having defined therein a plurality of vertical wells; (b) providing a layer of film disposed over said vertical wells to prevent the evaporation of liquid therefrom; (c) penetrating said layer of film, through slit means defined in said layer of film over at least one of said vertical wells, by at least one pipette tip to access said at least one of said vertical wells to add liquid to or withdraw liquid from said at least one of said vertical wells, said slit means defining a plurality of adjacent segments, and said layer of film being penetrated by said at least one pipette tip engaging edges of said segments and bending said edges downwardly toward said at least one of said vertical wells, thus forming an opening in said layer of film so that said at least one pipette tip accesses said at least one of said vertical wells; and (d) withdrawing said at least one pipette tip from said layer of film, with said layer of film resealing the area of penetration of said at least one pipette tip when said at least one pipette tip is withdrawn, by said edges returning to their original positions, in order to prevent evaporation of liquid from said at least one of said vertical wells.
Claim 2
Dependent Claim
221 words
A bioassay method, comprising: (a) providing a microplate having defined therein a plurality of vertical wells; (b) providing a layer of film disposed over said vertical wells to prevent the evaporation of liquid therefrom; (c) penetrating said layer of film, through slit means defined in said layer of film over at least one of said vertical wells, by at least one pipette tip to access said at least one of said vertical wells to add liquid to or withdraw liquid from said at least one of said vertical wells, said slit means defining a plurality of adjacent segments, and said layer of film being penetrated by said at least one pipette tip engaging edges of said segments and bending said edges downwardly toward said at least one of said vertical wells, thus forming an opening in said layer of film so that said at least one pipette tip accesses said at least one of said vertical wells; and (d) withdrawing said at least one pipette tip from said layer of film, with said layer of film resealing the area of penetration of said at least one pipette tip when said at least one pipette tip is withdrawn, by said edges returning to their original positions, in order to prevent evaporation of liquid from said at least one of said vertical wells.
Claim 3
Dependent Claim
31 words
A bioassay method, as defined in claim 1, further comprising: penetrating said layer of film simultaneously with a plurality of pipette tips to access any selected number of said vertical wells.
Claim 4
Dependent Claim
31 words
A bioassay method, as defined in claim 1, further comprising: attaching said layer of film to the upper surface of said microplate over said vertical wells with a pressure sensitive adhesive.
Claim 5
Dependent Claim
76 words
A bioassay method, as defined in claim 1, further comprising: penetrating said layer of film through slit means including a long slit defined through said film and extending about the length of the inside diameter of said at least one vertical well and a short slit defined through said film and extending about 75% of the inside diameter of said at least one vertical well, said long and said short slits being orthogonal to one another.
Claim 6
Dependent Claim
25 words
A bioassay method, as defined in claim 1, further comprising: providing said layer of film as clear polyester film in three to four mil thickness.
Claim 7
Dependent Claim
59 words
A bioassay method, as defined in claim 1, further comprising the step of moving said at least one pipette tip sideways along said slit means and within said at least one of said vertical wells to "touch off" the orifice of said at least one pipette tip on the sidewall of said at least one of said vertical walls.
Claim 8
Dependent Claim
109 words
A bioassay method, as defined in claim 1, further comprising: (a) providing said slit means defining a plurality of adjacent segments having apexes joined at a common point; (b) said layer of film is penetrated by said at least one pipette tip engaging said apexes and bending said apexes downwardly toward said at least one of said vertical wells, thus forming an opening in said layer of film; and (c) said layer of film is resealed to reseal the area of penetration of said at least one pipette tip when said at least one pipette tip is withdrawn, by said apexes of said segments returning to their original position.
Claim 9
Dependent Claim
214 words
A bioassay apparatus, comprising: (a) a microplate having defined therein a plurality of vertical wells; (b) a layer of film disposed over said vertical wells to prevent the evaporation of liquid therefrom, said layer of film being penetrable, through slit means defined in said layer of film over at least one of said vertical wells, by at least one pipette tip to access said at least one of said vertical wells to add liquid to or withdraw liquid from said at least one of said vertical wells; (c) said slit means defining a plurality of adjacent segments; (d) said layer of film being penetrable by said at least one pipette tip engaging edges of said segments and bending said edges downwardly toward said at least one of said vertical wells, thus forming an opening in said layer of film so that said at least one pipette tip can access said at least one of said vertical wells; and (e) said layer of film being resealable to reseal the area of penetration of said at least one pipette tip when said at least one pipette tip is withdrawn, by said edges of said segments returning to their original positions, in order to prevent evaporation of liquid from said at least one of said vertical wells.
Claim 10
Dependent Claim
214 words
A bioassay apparatus, comprising: (a) a microplate having defined therein a plurality of vertical wells; (b) a layer of film disposed over said vertical wells to prevent the evaporation of liquid therefrom, said layer of film being penetrable, through slit means defined in said layer of film over at least one of said vertical wells, by at least one pipette tip to access said at least one of said vertical wells to add liquid to or withdraw liquid from said at least one of said vertical wells; (c) said slit means defining a plurality of adjacent segments; (d) said layer of film being penetrable by said at least one pipette tip engaging edges of said segments and bending said edges downwardly toward said at least one of said vertical wells, thus forming an opening in said layer of film so that said at least one pipette tip can access said at least one of said vertical wells; and (e) said layer of film being resealable to reseal the area of penetration of said at least one pipette tip when said at least one pipette tip is withdrawn, by said edges of said segments returning to their original positions, in order to prevent evaporation of liquid from said at least one of said vertical wells.
Claim 11
Dependent Claim
31 words
A bioassay apparatus, as defined in claim 8, wherein: said layer of film is penetrable simultaneously by a plurality of pipette tips to access any selected number of said vertical wells.
Claim 12
Dependent Claim
31 words
A bioassay apparatus, as defined in claim 8, wherein: said layer of film is attached to the upper surface of said microplate over said vertical wells with a pressure sensitive adhesive.
Claim 13
Dependent Claim
70 words
A bioassay apparatus, as defined in claim 8, wherein: said slit means includes a long slit defined through said film and extending about the length of the inside diameter of said at least one vertical well and a short slit defined through said film and extending about 75% of the inside diameter of said at least one vertical well, said long and said short slits being orthogonal to one another.
Claim 14
Dependent Claim
23 words
A bioassay apparatus, as defined in claim 8, wherein: said layer of film is clear polyester film in three to four mil thickness.
Claim 15
Dependent Claim
107 words
A bioassay apparatus, as defined in claim 8, wherein: (a) said slit means defines a plurality of adjacent segments having apexes joined at a common point; (b) said layer of film is penetrable by said at least one pipette tip engaging said apexes and bending said apexes downwardly toward said at least one of said vertical wells, thus forming an opening in said layer of film; and (c) said layer of film is resealable to reseal the area of penetration of said at least one pipette tip when said at least one pipette tip is withdrawn, by said apexes of said segments returning to their original position.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2001-08-03 | 2002-02-02 | 2002-08-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2005-08-03 | 2006-02-02 | 2006-08-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2009-08-03 | 2010-02-02 | 2010-08-04 | $7700.00 | Window Closed / Lapsed |