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US5789158A Expired / Lapsed US ⏱️ 0.0 years remaining

Developmental embryonic mouse cDNA libraries

Assignee: The Wistar Institute
•
Filing: 1995-05-18
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Granted: 1998-08-04
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Expires: 2015-05-18
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Abstract

Abstract The present invention provides a number of cDNA libraries constructed from unfertilized eggs and 2-cell, 8-cell and blastocyst stage embryos, as well as a number of novel genes expressed in the 2-cell libraries.

Inventors

  • 👤 Barbara B. Knowles
  • 👤 Jay L. Rothstein
  • 👤 Dabney Johnson
  • 👤 Davor Solter

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-11-03 10:57
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6876; C12N; C12N15/00; C12N15/09; C12N15/10; C12N15/1096; C12Q1/6813; C12Q2600/00; C12Q2600/158

Claims Analysis

Total Claims: 14
Claim 1
Independent Claim 10 words
Murine embryonic cDNA library constructed from unfertilized eggs ATCC #69022.
Claim 2
Dependent Claim 10 words
Murine embryonic cDNA library constructed from unfertilized eggs ATCC #69022.
Claim 3
Dependent Claim 12 words
Murine embryonic cDNA library constructed from 2-cell stage preimplantation embryos ATCC #69027.
Claim 4
Dependent Claim 12 words
Murine embryonic cDNA library constructed from 2-cell stage preimplantation embryos ATCC #69027.
Claim 5
Dependent Claim 12 words
Murine embryonic cDNA library constructed from 8-cell stage preimplantation embryos ATCC #69024.
Claim 6
Dependent Claim 12 words
Murine embryonic cDNA library constructed from 8-cell stage preimplantation embryos ATCC #69024.
Claim 7
Dependent Claim 11 words
Murine embryonic cDNA library constructed from blastocyst-stage preimplantation embryos ATCC #69026.
Claim 8
Dependent Claim 11 words
Murine embryonic cDNA library constructed from blastocyst-stage preimplantation embryos ATCC #69026.
Claim 9
Dependent Claim 9 words
Murine embryonic subtraction cDNA library designated 2CLS-I ATCC #69025.
Claim 10
Dependent Claim 9 words
Murine embryonic subtraction cDNA library designated 2CLS-I ATCC #69025.
Claim 11
Dependent Claim 9 words
Murine embryonic subtraction cDNA library designated 2CLS-II ATCC #69023.
Claim 12
Dependent Claim 9 words
Murine embryonic subtraction cDNA library designated 2CLS-II ATCC #69023.
Claim 13
Independent Claim 78 words
A method for identifying a novel gene or gene fragment comprising the steps of: synthesizing a DNA probe comprising a DNA sequence homologous to at least one DNA sequence within a murine embryonic cDNA library selected from the group consisting of UESL ATCC #69022, BSL ATCC #69026, 2CSL ATCC #69027, and 8CSL ATCC #69024, labeling the DNA probe, and hybridizing the labeled DNA probe to the selected library, said label permitting identification of the gene or gene fragment.
Claim 14
Independent Claim 78 words
A method for identifying a novel gene or gene fragment comprising the steps of: synthesizing a DNA probe comprising a DNA sequence homologous to at least one DNA sequence within a murine embryonic cDNA library selected from the group consisting of UESL ATCC #69022, BSL ATCC #69026, 2CSL ATCC #69027, and 8CSL ATCC #69024, labeling the DNA probe, and hybridizing the labeled DNA probe to the selected library, said label permitting identification of the gene or gene fragment.

References Cited (Backward Citations)

EP0154186A2 Cited
EP0242991A2 Cited
EP0354624A2 Cited
WO1991017271A1 Cited

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 2001-08-03 2002-02-02 2002-08-04 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 2005-08-03 2006-02-02 2006-08-04 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 2009-08-03 2010-02-02 2010-08-04 $7700.00 Window Closed / Lapsed

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