US5783183A
Expired / Lapsed
US
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Cloning of the zymocin gene and use of zymocin in beverages
Assignee: DSM IP Assets BV
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Filing: 1994-03-03
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Granted: 1998-07-21
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Expires: 2014-03-03
Abstract
Abstract PCT No. PCT/EP94/00634 Sec. 371 Date Feb. 22, 1995 Sec. 102(e) Date Feb. 22, 1995 PCT Filed Mar. 3, 1994 PCT Pub. No. WO94/20620 PCT Pub. Date Sep. 15, 1994The present invention discloses a method for the preservation of beverages comprising the use of a killer toxin. Specifically, zymocin is used in carbonated drinks. The zymocin is shown to be active against a wide range of different yeast strains. The invention further discloses a DNA fragment encoding zymocin. The DNA sequence is also disclosed.
Inventors
- 👤 Pieter Cornelis Langeveld
- 👤 Pieter Van Solingen
- 👤 Jacobus Stark
- 👤 Adrianus Wilhelmus Hermanus Vollebregt
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 22:03
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/37; C07K14/39
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
19 words
A method for preserving non-alcoholic, non-carbonated beverages comprising adding an effective amount of a yeast zymocin to said beverage.
Claim 2
Independent Claim
19 words
A method for preserving non-alcoholic, non-carbonated beverages comprising adding an effective amount of a yeast zymocin to said beverage.
Claim 3
Dependent Claim
18 words
A method according to claim 1 wherein said non-carbonated beverage contains fruit, fruit juice or a fruit extract.
Claim 4
Dependent Claim
26 words
A method according to claim 2 wherein the beverage is selected from the group consisting of apple juice, orange juice, pear juice and black currant drink.
Claim 5
Dependent Claim
24 words
A method according to claim 1 wherein said zymocin is obtainable from a yeast strain selected from a genus consisting of Hansenula and Williopsis.
Claim 6
Dependent Claim
21 words
A method according to claim 1 wherein said zymocin is added to achieve a final concentration of 128 to 512 AU/ml.
Claim 7
Dependent Claim
27 words
A method according to claim 5 wherein said zymocin is produced from an organism that has been altered to contain a DNA molecule that encodes said zymocin.
Claim 8
Dependent Claim
23 words
A method according to claim 6 wherein said DNA molecule encodes a zymocin having the amino acid sequence depicted in Sequence ID No:5.
Claim 9
Dependent Claim
13 words
A non-alcoholic, non-carbonated beverage having been preserved by the method of claim 5.
References Cited (Backward Citations)
US4695468A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2001-07-20 | 2002-01-19 | 2002-07-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2005-07-20 | 2006-01-19 | 2006-07-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2009-07-20 | 2010-01-19 | 2010-07-21 | $7700.00 | Window Closed / Lapsed |