US5773577A
Expired / Lapsed
US
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Products comprising substrates capable of enzymatic cross-linking
Assignee: Protein Polymer Technologies Inc
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Filing: 1995-03-02
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Granted: 1998-06-30
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Expires: 2015-03-02
Abstract
Abstract Polymers are provided comprising protein polymers comprising blocks of repeating units and sequences comprising amino acids, individually or in defined sequences, capable of enzyme catalyzed covalent bond formation for cross-linking, as exemplified by glutamine and/or lysine reactive for FXIII catalyzed isopeptide formation or non-amino acid polymers having side chains comprising such amino acids or sequences, which may be used for preparation of articles of manufacture, particularly cross-linkable compositions. By appropriate choice of the polymer, resorbable implantable polymers may be used in internal applications for mammals as formed objects or depots.
Inventors
- 👤 Joseph Cappello
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 21:49
CPC Classifications
A; A61; A61L; A61L24/00; A61L24/04; A61L24/10; A61L24/106; A61P; A61P17/00; A61P17/02; A61P7/00; A61P7/04; C; C07; C07K; C07K14/00; C07K14/435; C07K14/46; C07K14/47; C07K14/4701; C07K14/4732; C12; C12N; C12N9/00; C12N9/10
Claims Analysis
Total Claims: 35
Claim 1
Independent Claim
55 words
A recombinant protein polymer of a molecular weight in the range of 15 to 250 kD comprised of naturally occurring repetitive units from 3 to 18 amino acids and at least two enzyme recognition sequences separated by at least 25 intervening amino acids, said recognition sequences comprising a glutamine capable of enzyme catalyzed isopeptide formation.
Claim 2
Dependent Claim
55 words
A recombinant protein polymer of a molecular weight in the range of 15 to 250 kD comprised of naturally occurring repetitive units from 3 to 18 amino acids and at least two enzyme recognition sequences separated by at least 25 intervening amino acids, said recognition sequences comprising a glutamine capable of enzyme catalyzed isopeptide formation.
Claim 3
Dependent Claim
23 words
A recombinant protein polymer according to claim 1, comprising at least three of said enzyme recognition sequences, separated by the same intervening sequence.
Claim 4
Dependent Claim
33 words
A recombinant protein polymer according to claim 2, wherein the intervening sequences comprise a naturally occurring functional sequence selected from the group consisting of the fibronectin binding site and the laminin binding site.
Claim 5
Dependent Claim
23 words
A recombinant protein polymer according to claim 1, wherein said repetitive units have the collagen motif of every third amino acid being glycine.
Claim 6
Dependent Claim
22 words
A recombinant protein polymer according to claim 1, wherein said repetitive units consist of at least one fibroin or elastin repetitive unit.
Claim 7
Dependent Claim
71 words
A recombinant protein polymer of from 35 kD to 250 kD comprising as a backbone alternating sequences comprising (1) repetitive units having the collagen motif of every third amino acid being glycine and (2) enzyme recognition sequences of from about 3 to 60 amino acids, said enzyme recognition sequences comprising an amino acid residue selected from the group consisting of glutamine and lysine which is capable of enzyme catalyzed isopeptide formation.
Claim 8
Dependent Claim
71 words
A recombinant protein polymer of from 35 kD to 250 kD comprising as a backbone alternating sequences comprising (1) repetitive units having the collagen motif of every third amino acid being glycine and (2) enzyme recognition sequences of from about 3 to 60 amino acids, said enzyme recognition sequences comprising an amino acid residue selected from the group consisting of glutamine and lysine which is capable of enzyme catalyzed isopeptide formation.
Claim 9
Dependent Claim
19 words
A recombinant protein polymer according to claim 6, wherein said enzyme recognition sequences further comprise a fibrin gamma polsite.
Claim 10
Dependent Claim
47 words
A recombinant protein polymer of a molecular weight in the range of 15 to 250 kD comprising naturally occurring repetitive units from 3 to 18 amino acids and at least 2 pendent groups, said pendent groups comprising a glutamine and/or lysine capable of enzyme catalyzed isopeptide formation.
Claim 11
Dependent Claim
47 words
A recombinant protein polymer of a molecular weight in the range of 15 to 250 kD comprising naturally occurring repetitive units from 3 to 18 amino acids and at least 2 pendent groups, said pendent groups comprising a glutamine and/or lysine capable of enzyme catalyzed isopeptide formation.
Claim 12
Dependent Claim
36 words
A recombinant protein polymer according to claim 8, wherein said polymer is collagen and said pendent groups are consensus sequences from casein or fibrin, said consensus sequences having the reactive lysine substituted with another amino acid.
Claim 13
Dependent Claim
16 words
A recombinant protein polymer according to claim 8, wherein said polymer is at least 35 kD.
Claim 14
Dependent Claim
23 words
A recombinant protein polymer according to claim 8, wherein said repetitive units have the collagen motif of every third amino acid being glycine.
Claim 15
Dependent Claim
23 words
A recombinant protein polymer according to claim 8, wherein said repetitive units consist of at least one of fibroin or elastin repetitive units.
Claim 16
Dependent Claim
19 words
A recombinant protein polymer according to claim 8, wherein said pendent groups comprise the sequence Leu-Gly-Pro-Gly-Gln-Ser-Lys-Val-Ile-Gly (SEQ ID NO:48).
Claim 17
Dependent Claim
45 words
A recombinant protein polymer of a molecular weight in the range of 35 to 250 kD comprising naturally occurring repetitive units from 3 to 18 amino acids and at least 2 pendent groups, said pendent groups comprising a lysine capable of enzyme catalyzed isopeptide formation.
Claim 18
Dependent Claim
45 words
A recombinant protein polymer of a molecular weight in the range of 35 to 250 kD comprising naturally occurring repetitive units from 3 to 18 amino acids and at least 2 pendent groups, said pendent groups comprising a lysine capable of enzyme catalyzed isopeptide formation.
Claim 19
Dependent Claim
19 words
A recombinant protein polymer according to claim 14, wherein said pendent groups comprise the sequence Gly-Gly-Leu-Lys-Gly-Gly-Gly (SEQ ID NO:71).
Claim 20
Dependent Claim
39 words
A composition comprising a recombinant protein polymer according to claim 1 and a cross-linking compound other than said recombinant protein polymer said cross-linking compound comprising at least two reactive groups capable of enzyme catalyzed isopeptide formation with said glutamine.
Claim 21
Dependent Claim
19 words
A composition according to claim 18, wherein said cross-linking compound is a protein polymer comprising a plurality of lysines.
Claim 22
Dependent Claim
25 words
A composition according to claim 18, wherein said cross-linking compound is a molecule of less than 5 kD comprising at least two primary amino groups.
Claim 23
Dependent Claim
17 words
A composition comprising a recombinant protein polymer according to claim 1 and Factor XIII or Factor XIIla.
Claim 24
Dependent Claim
64 words
A recombinant protein polymer of from 15 to 250 kD, comprising a repetitive amino acid backbone of repetitive units having the collagen motif of every third amino acid being glycine, fibroin motif, elastin motif or keratin motif, and at least two enzyme recognition sequences comprising a glutamine capable of enzyme catalyzed isopeptide formation, separated by an intervening sequence of at least 25 amino acids.
Claim 25
Dependent Claim
64 words
A recombinant protein polymer of from 15 to 250 kD, comprising a repetitive amino acid backbone of repetitive units having the collagen motif of every third amino acid being glycine, fibroin motif, elastin motif or keratin motif, and at least two enzyme recognition sequences comprising a glutamine capable of enzyme catalyzed isopeptide formation, separated by an intervening sequence of at least 25 amino acids.
Claim 26
Dependent Claim
19 words
A recombinant protein polymer according to claim 20, wherein said enzyme recognition sequence is in said amino acid backbone.
Claim 27
Dependent Claim
20 words
A recombinant protein polymer according to claim 20, wherein said enzyme recognition sequence is pendent from said amino acid backbone.
Claim 28
Dependent Claim
23 words
A recombinant protein polymer according to claim 20, wherein said repetitive unit has the collagen motif of every third amino acid being glycine.
Claim 29
Dependent Claim
18 words
A recombinant protein polymer according to claim 23, wherein said enzyme recognition sequence is the casein recognition sequence.
Claim 30
Dependent Claim
18 words
A recombinant protein polymer according to claim 20, further comprising a fibrin gamma polsite in an intervening sequence.
Claim 31
Dependent Claim
64 words
A recombinant protein polymer of from 35 to 250 kD, comprising a repetitive amino acid backbone of repetitive units having the collagen motif of every third amino acid being glycine and being from 10 to 45% proline, and at least two enzyme recognition sequences comprising a glutamine capable of enzyme catalyzed isopeptide formation, separated by an intervening sequence of at least 25 amino acids.
Claim 32
Dependent Claim
64 words
A recombinant protein polymer of from 35 to 250 kD, comprising a repetitive amino acid backbone of repetitive units having the collagen motif of every third amino acid being glycine and being from 10 to 45% proline, and at least two enzyme recognition sequences comprising a glutamine capable of enzyme catalyzed isopeptide formation, separated by an intervening sequence of at least 25 amino acids.
Claim 33
Dependent Claim
19 words
A recombinant protein polymer according to claim 26, wherein said enzyme recognition sequence is the fibrinogen or casein sequence.
Claim 34
Dependent Claim
22 words
A recombinant protein polymer according to claim 27, wherein lysine present in said enzyme recognition sequence is substituted with another amino acid.
Claim 35
Dependent Claim
17 words
A recombinant protein polymer according to claim 26, wherein said intervening sequence comprises a fibrin gamma polsite.
References Cited (Backward Citations)
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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