US5773268A
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Chromosome 21 gene marker, compositions and methods using same
Assignee: Cedars-Sinai Medical Center
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Filing: 1994-11-09
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Granted: 1998-06-30
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Expires: 2014-11-09
Abstract
Abstract The present invention provides isolated nucleic acids encoding human EHOC-1 protein and isolated receptor proteins encoded thereby. Further provided are vectors containing invention nucleic acids, probes that hybridize thereto, host cells transformed therewith, antisense oligonucleotides thereto and compositions containing, antibodies that specifically bind to invention polypeptides and compositions containing, as well as transgenic non-human mammals that express the invention protein.
Inventors
- 👤 Julie R. Korenberg
- 👤 Kazuhiro Yamakawa
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 11:48
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/46; C07K14/47; A; A01; A01K; A01K2217/00; A01K2217/05; A61; A61K; A61K48/00
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
20 words
An isolated polynucleotide encoding an epilepsy holoprosencephaly related protein, comprising a nucleic acid encoding amino acid SEQ ID No: 2.
Claim 2
Dependent Claim
20 words
An isolated polynucleotide encoding an epilepsy holoprosencephaly related protein, comprising a nucleic acid encoding amino acid SEQ ID No: 2.
Claim 3
Dependent Claim
8 words
The polynucleotide of claim 1, being a deoxyribonucleotide.
Claim 4
Dependent Claim
17 words
The polynucleotide of claim 1, wherein the nucleic acid comprises nucleotides 157-3726 of SEQ ID NO: 1.
Claim 5
Dependent Claim
14 words
A polynucleotide, comprising a nucleic acid complementary to the nucleic acid of claim 1.
Claim 6
Dependent Claim
9 words
The complementary polynucleotide of claim 4, being a ribonucleotide.
Claim 7
Dependent Claim
13 words
The polynucleotide of claim 1, operably linked to a promoter of RNA transcription.
Claim 8
Dependent Claim
9 words
The polynucleotide of claim 1, in double stranded form.
Claim 9
Dependent Claim
12 words
A composition, comprising the isolated polynucleotide of claim 1, and a carrier.
Claim 10
Dependent Claim
14 words
A cloning vector, having the polynucleotide of claim 1 operably linked to said vector.
Claim 11
Dependent Claim
17 words
The vector of claim 9, wherein the polynucleotide is operably linked in reading frame to the vector.
Claim 12
Dependent Claim
12 words
The vector of claim 10, wherein said vector is an expression vector.
Claim 13
Dependent Claim
11 words
A composition, comprising the vector of claim 9, and a carrier.
Claim 14
Dependent Claim
14 words
A host cell culture, comprising host cells transfected with the vector of claim 10.
Claim 15
Dependent Claim
12 words
The cell culture of claim 13, wherein the cells are prokaryotic cells.
Claim 16
Dependent Claim
12 words
The cell culture of claim 13, wherein the cells are eukaryotic cells.
Claim 17
Dependent Claim
17 words
The cell culture of claim 13, wherein the cells express the amino acid SEQ ID No: 2.
Claim 18
Dependent Claim
12 words
A composition, comprising the cell culture of claim 13, and a carrier.
Claim 19
Dependent Claim
13 words
A composition, comprising the cell culture of claim 13, and a culture medium.
Claim 20
Dependent Claim
66 words
A method of introducing a wild type nucleic acid sequence at chromosome 21q22.3, comprising obtaining cells from a subject lacking two wild type chromosomes 21q22.3; transforming the subject's cells ex-vivo with the polynucleotide of claim 1, and a selection marker nucleic acid; selecting the transformed cells in a selection medium; and isolating viable transformed cells carrying the introduced polynucleotide from the medium and the remainder cells.
References Cited (Backward Citations)
WO1993010228A1
Cited
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