US5770439A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Thick-film conductivity enzyme electrodes in a vertical stack as biosensor
Assignee: Helmholtz Centre for Infection Research
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Filing: 1992-09-28
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Granted: 1998-06-23
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Expires: 2012-09-28
Abstract
Abstract A biosensor is produced by depositing thick film pastes by screen-printing on a substrate to produce thick-film conductivity electrodes, and immobilizing enzymes on the electrodes. In specific embodiments, the substrate is an unfired ceramic, a fired ceramic or a plastic, the paste is a noble metal paste, enzyme immobilization is with a bifunctional reagent, a plurality of different enzymes are immobilized on different electrodes, and/or the electrodes are printed as parallel lines or in the image of a comb or concentric circles. A multi-dimensional biosensor is prepared by forming thick film paste electrodes containing an immobilized enzyme on a plurality of separate substrates, disposing the separate substrates one on top of the other in a vertical stack, pressing the stacked substrates together and curing the paste.
Inventors
- 👤 Ursula Bilitewski
- 👤 Wiebke Drewes
- 👤 Franz Bechtold
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 17:54
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/002; C12Q1/003; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
101 words
A method for the production of a conductivity biosensor which comprises; a plurality of electrodes on a plurality of substrates; and an enzyme immobilized on the electrodes; comprising the steps; A. screen printing on a plurality of separate substrates a conductive electrode forming thick film paste, whereby the electrode is formed on the substrate; B. immobilizing an enzyme on the electrode; C. disposing the separate substrates bearing printed conductive electrodes and immobilized enzyme in a vertical stack, one on top of the other; D. pressing the stacked substrates together; and E. curing the printed paste, whereby a multi-dimensional biosensor is produced.
Claim 2
Independent Claim
101 words
A method for the production of a conductivity biosensor which comprises; a plurality of electrodes on a plurality of substrates; and an enzyme immobilized on the electrodes; comprising the steps; A. screen printing on a plurality of separate substrates a conductive electrode forming thick film paste, whereby the electrode is formed on the substrate; B. immobilizing an enzyme on the electrode; C. disposing the separate substrates bearing printed conductive electrodes and immobilized enzyme in a vertical stack, one on top of the other; D. pressing the stacked substrates together; and E. curing the printed paste, whereby a multi-dimensional biosensor is produced.
Claim 3
Dependent Claim
12 words
The method of claim 1 wherein the substrate is an unfired ceramic.
Claim 4
Dependent Claim
21 words
The method of claim 1 wherein the substrate is selected from the group consisting of a fired ceramic and a plastic.
Claim 5
Dependent Claim
13 words
The method of claim 1 wherein the paste is a noble metal paste.
Claim 6
Dependent Claim
18 words
The method of claim 1 wherein the enzyme is one which catalyzes a reaction wherein ions are released.
Claim 7
Dependent Claim
16 words
The method of claim 1 wherein a plurality of different enzymes are immobilized on different electrodes.
Claim 8
Dependent Claim
14 words
The method of claim 1 wherein immobilization is by cross-linking with a bifunctional reagent.
Claim 9
Dependent Claim
47 words
A conductivity biosensor, which comprises; A. a plurality of substrate layers pressed together in a vertical stack; B. each substrate layer bearing a conductive electrode formed thereon by screen printing a thick film paste; and C. each electrode having immobilized thereon an enzyme for sensing an analyte.
Claim 10
Dependent Claim
47 words
A conductivity biosensor, which comprises; A. a plurality of substrate layers pressed together in a vertical stack; B. each substrate layer bearing a conductive electrode formed thereon by screen printing a thick film paste; and C. each electrode having immobilized thereon an enzyme for sensing an analyte.
Claim 11
Dependent Claim
14 words
The biosensor of claim 8 wherein the electrodes are positioned in different geometric forms.
Claim 12
Dependent Claim
13 words
The biosensor of claim 8 wherein the electrodes are printed as parallel lines.
Claim 13
Dependent Claim
16 words
The biosensor of claim 8 wherein the electrodes are printed in the image of a comb.
Claim 14
Dependent Claim
16 words
The biosensor of claim 8 wherein the electrodes are printed in the image of concentric circles.
Claim 15
Dependent Claim
14 words
The biosensor of claim 8 wherein immobilization is by cross-linking with a bifunctional reagent.
Claim 16
Dependent Claim
93 words
A conductivity biosensor for detecting the presence of a plurality of different analytes, which comprises; A. a plurality of substrate layers pressed together in a vertical stack; B. each substrate layer bearing on the surface thereof, a conductive electrode formed by a screen-printing of a thick-film paste of electrode forming composition; C. each electrode having immobilized thereon an enzyme for sensing at least one of said analytes; D. at least two of the electrodes having enzymes for detecting different analytes; and E. conductor means attached to the electrodes for supplying electrical current thereto.
Claim 17
Dependent Claim
93 words
A conductivity biosensor for detecting the presence of a plurality of different analytes, which comprises; A. a plurality of substrate layers pressed together in a vertical stack; B. each substrate layer bearing on the surface thereof, a conductive electrode formed by a screen-printing of a thick-film paste of electrode forming composition; C. each electrode having immobilized thereon an enzyme for sensing at least one of said analytes; D. at least two of the electrodes having enzymes for detecting different analytes; and E. conductor means attached to the electrodes for supplying electrical current thereto.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2001-06-22 | 2001-12-22 | 2002-06-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2005-06-22 | 2005-12-22 | 2006-06-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2009-06-22 | 2009-12-22 | 2010-06-23 | $7700.00 | Window Closed / Lapsed |