US5766905A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Cytoplasmic bacteriophage display system
Assignee: Brookhaven Science Associates LLC
•
Filing: 1996-06-17
•
Granted: 1998-06-16
•
Expires: 2016-06-17
Abstract
Abstract Disclosed are display vectors comprising DNA encoding a portion of a structural protein from a cytoplasmic bacteriophage, joined covalently to a protein or peptide of interest. Exemplified are display vectors wherein the structural protein is the T7 bacteriophage capsid protein. More specifically, in the exemplified display vectors the C-terminal amino acid residue of the portion of the capsid protein is joined to the N-terminal residue of the protein or peptide of interest. The portion of the T7 capsid protein exemplified comprises an N-terminal portion corresponding to form 10B of the T7 capsid protein. The display vectors are useful for high copy number display or lower copy number display (with larger fusion). Compositions of the type described herein are useful in connection with methods for producing a virus displaying a protein or peptide of interest.
Inventors
- 👤 F. William Studier
- 👤 Alan H. Rosenberg
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 14:40
CPC Classifications
C; C40; C40B; C40B40/00; C40B40/02; C07; C07K; C07K16/00; C07K16/16; C12; C12N; C12N15/00; C12N15/09; C12N15/10; C12N15/1034; C12N15/1037
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
41 words
A bacteriophage T7 display vector comprising DNA encoding at least residues 1 through 341 of the bacteriophage T7 capsid protein followed by a cloning site for the in-frame insertion of a foreign DNA sequence encoding a peptide or protein of interest.
Claim 2
Dependent Claim
41 words
A bacteriophage T7 display vector comprising DNA encoding at least residues 1 through 341 of the bacteriophage T7 capsid protein followed by a cloning site for the in-frame insertion of a foreign DNA sequence encoding a peptide or protein of interest.
Claim 3
Dependent Claim
24 words
The bacteriophage T7 display vector of claim 1 further comprising amino acid residues 342-348 of the 10B form of the bacteriophage T7 capsid protein.
Claim 4
Dependent Claim
16 words
The bacteriophage T7 display vector of claim 1, further comprising wild-type T7 capsid protein regulatory signals.
Claim 5
Dependent Claim
21 words
The bacteriophage T7 display vector of claim 3, wherein the wild-type capsid protein regulatory signals comprise promoter and translation initiation signals.
Claim 6
Dependent Claim
26 words
The bacteriophage T7 display vector of claim 4, wherein the promoter is the φ10 promoter and the translation initiation signal is the s10 translation initiation signal.
Claim 7
Dependent Claim
15 words
The bacteriophage T7 display vector of claim 5, further comprising the wild type Tφ terminator.
Claim 8
Dependent Claim
18 words
The bacteriophage T7 display vector of claim 2 which lacks wild-type capsid protein promoter and translation initiation signals.
Claim 9
Dependent Claim
44 words
A cell containing a bacteriophage T7 display vector comprising DNA encoding at least residues 1 through 341 of the bacteriophage T7 capsid protein followed by a cloning site for the in-frame insertion of a foreign DNA sequence encoding a peptide or protein of interest.
Claim 10
Dependent Claim
44 words
A cell containing a bacteriophage T7 display vector comprising DNA encoding at least residues 1 through 341 of the bacteriophage T7 capsid protein followed by a cloning site for the in-frame insertion of a foreign DNA sequence encoding a peptide or protein of interest.
Claim 11
Dependent Claim
27 words
The cell of claim 8 wherein the bacteriophage T7 display vector further comprises amino acid residues 342-348 of the 10B form of the bacteriophage T7 capsid protein.
Claim 12
Dependent Claim
13 words
The cell of claim 8, further comprising wild-type T7 capsid protein regulatory signals.
Claim 13
Dependent Claim
18 words
The cell of claim 10, wherein the wild-type capsid protein regulatory signals comprise promoter and translation initiation signals.
Claim 14
Dependent Claim
23 words
The cell of claim 11, wherein the promoter is the φ10 promoter and the translation initiation signal is the s10 translation initiation signal.
Claim 15
Dependent Claim
12 words
The cell of claim 12, further comprising the wild type Tφ terminator.
Claim 16
Dependent Claim
15 words
The cell of claim 8 which lacks wild-type capsid protein promoter and translation initiation signals.
Claim 17
Dependent Claim
38 words
A viral lysate containing assembled bacteriophage T7 particles, the assembled bacteriophage T7 particles containing a fusion protein comprising at least residues 1 through 341 of the bacteriophage T7 capsid protein fused to a peptide or protein of interest.
Claim 18
Dependent Claim
38 words
A viral lysate containing assembled bacteriophage T7 particles, the assembled bacteriophage T7 particles containing a fusion protein comprising at least residues 1 through 341 of the bacteriophage T7 capsid protein fused to a peptide or protein of interest.
Claim 19
Dependent Claim
28 words
The viral lysate of claim 15 wherein the assembled bacteriophage T7 particles further comprise amino acid residues 342-348 of the 10B form of the bacteriophage T7 capsid protein.
Claim 20
Dependent Claim
41 words
A bacteriophage T3 display vector comprising DNA encoding at least residues 1 through 341 of the bacteriophage T7 capsid protein followed by a cloning site for the in-frame insertion of a foreign DNA sequence encoding a peptide or protein of interest.
Claim 21
Dependent Claim
41 words
A bacteriophage T3 display vector comprising DNA encoding at least residues 1 through 341 of the bacteriophage T7 capsid protein followed by a cloning site for the in-frame insertion of a foreign DNA sequence encoding a peptide or protein of interest.
Claim 22
Dependent Claim
24 words
The bacteriophage T3 display vector of claim 17 further comprising amino acid residues 342-348 of the 10B form of the bacteriophage T7 capsid protein.
References Cited (Backward Citations)
US5550035A
Cited
US5534257A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2001-06-15 | 2001-12-15 | 2002-06-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2005-06-15 | 2005-12-15 | 2006-06-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2009-06-15 | 2009-12-15 | 2010-06-16 | $7700.00 | Window Closed / Lapsed |