Patent US1435029A successfully refreshed from Google Patents.
US5763583A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for producing solubilized protein
Assignee: Kao Corp
•
Filing: 1995-10-06
•
Granted: 1998-06-09
•
Expires: 2015-10-06
Abstract
Abstract A process for the production of a solubilized protein is disclosed, which is obtained by reducing disulfide bonds in a disulfide bond-containing water-insoluble protein material into mercapto groups and subsequently converting a part or an entire portion thereof into carboxymethyldisulfide groups. In particular, the disclosed process for the production of a solubilized protein which comprises (a) treating a disulfide bond-containing water-insoluble protein material with an aqueous alkaline solution of a reducing agent, and (b) reacting the protein treated by step (a) with thioglycolic acid in the presence of an oxidizing agent under a weakly acidic to a weakly alkaline condition. A process for the production of regenerated protein products, which comprises regenerating disulfide bonds in the solubilized protein, is also disclosed.
Inventors
- 👤 Kozo Arai
- 👤 Hiroshi Nojiri
- 👤 Sachio Naito
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 22:58
CPC Classifications
C; C07; C07K; C07K1/00; C07K1/107; C07K1/113; C07K1/1136; C07K1/1072; C07K1/1077; C07K1/1133; C07K14/00; C07K14/435; C07K14/46; C07K14/47; C07K14/4701; C07K14/4741
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
98 words
A process for the production of a solubilized protein which consists essentially of: (a) treating a disulfide bond-containing water-insoluble protein material with an aqueous alkaline solution of a reducing agent and then treating with a protein denaturing agent; and (b) reacting said disulfide bond-containing water-insoluble protein material from step (a) with thioglycolic acid in the presence of an oxidizing agent under a weakly acidic to a weakly alkaline condition to convert disulfide bonds in said disulfide bond-containing water-insoluble protein material into carboxymethyldisulfide groups and thereby produce said solubilized protein, said process being conducted without any water washing step.
Claim 2
Dependent Claim
98 words
A process for the production of a solubilized protein which consists essentially of: (a) treating a disulfide bond-containing water-insoluble protein material with an aqueous alkaline solution of a reducing agent and then treating with a protein denaturing agent; and (b) reacting said disulfide bond-containing water-insoluble protein material from step (a) with thioglycolic acid in the presence of an oxidizing agent under a weakly acidic to a weakly alkaline condition to convert disulfide bonds in said disulfide bond-containing water-insoluble protein material into carboxymethyldisulfide groups and thereby produce said solubilized protein, said process being conducted without any water washing step.
Claim 3
Dependent Claim
12 words
The process of claim 1, wherein said reducing agent comprises thioglycolic acid.
Claim 4
Dependent Claim
13 words
The process of claim 1, wherein said oxidizing agent comprises air or oxygen.
Claim 5
Dependent Claim
27 words
The process of claim 1, wherein said disulfide bond-containing water-insoluble protein material is selected from the group consisting of hairs, feathers, horns, hooves, and claws of animals.
Claim 6
Dependent Claim
130 words
A process for the production of a regenerated protein product from a solubilized protein which consists essentially of: (a) treating a disulfide bond-containing water-insoluble protein material with an aqueous alkaline solution of a reducing agent and then treating with a protein denaturing agent; (b) reacting said disulfide bond-containing water-insoluble protein material from step (a) with thioglycolic acid in the presence of an oxidizing agent under a weakly acidic to weakly alkaline condition to convert disulfide bonds in said disulfide bond-containing water-insoluble protein material into carboxymethyldisulfide groups and thereby produce said solubilized protein; (c) recovering said solubilized protein; and (d) forming said solubilized protein into a shape; and (e) regenerating disulfide bonds in said solubilized protein to produce said regenerated protein product, said process being conducted without any water washing step.
Claim 7
Dependent Claim
130 words
A process for the production of a regenerated protein product from a solubilized protein which consists essentially of: (a) treating a disulfide bond-containing water-insoluble protein material with an aqueous alkaline solution of a reducing agent and then treating with a protein denaturing agent; (b) reacting said disulfide bond-containing water-insoluble protein material from step (a) with thioglycolic acid in the presence of an oxidizing agent under a weakly acidic to weakly alkaline condition to convert disulfide bonds in said disulfide bond-containing water-insoluble protein material into carboxymethyldisulfide groups and thereby produce said solubilized protein; (c) recovering said solubilized protein; and (d) forming said solubilized protein into a shape; and (e) regenerating disulfide bonds in said solubilized protein to produce said regenerated protein product, said process being conducted without any water washing step.
References Cited (Backward Citations)
US4652630A
Cited
US4694073A
Cited
US4731440A
Cited
US4652630B1
Cited
WO1987002985A1
Cited
US4985544A
Cited
US5240834A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2001-06-08 | 2001-12-08 | 2002-06-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2005-06-08 | 2005-12-08 | 2006-06-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2009-06-08 | 2009-12-08 | 2010-06-09 | $7700.00 | Window Closed / Lapsed |