US5763239A
Expired / Lapsed
US
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Production and use of normalized DNA libraries
Assignee: BASF Enzymes LLC
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Filing: 1996-06-18
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Granted: 1998-06-09
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Expires: 2016-06-18
Abstract
Abstract Disclosed is a process for forming a normalized genomic DNA library from an environmental sample by (a) isolating a genomic DNA population from the environmental sample; (b) analyzing the complexity of the genomic DNA population so isolated; (c) at least one of (i) amplifying the copy number of the DNA population so isolated and (ii) recovering a fraction of the isolated genomic DNA having a desired characteristic; and (d) normalizing the representation of various DNAs within the genomic DNA population so as to form a normalized library of genomic DNA from the environmental sample. Also disclosed is a normalized genomic DNA library formed from an environmental sample by the process.
Inventors
- 👤 Jay M. Short
- 👤 Eric J. Mathur
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 03:42
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/10; C12N15/1034; C12N15/1093; C12N15/1096; C12Q; C12Q1/00; C12Q1/68; C12Q1/6811
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
104 words
A process for forming a normalized genomic DNA library from an environmental sample, which comprises the steps of: (a) isolating a genomic DNA population from the environmental sample; (b) analyzing the complexity of the genomic DNA population so isolated; (c) at least one of the steps selected from the group consisting of (i) amplifying the copy number of the DNA population so isolated and (ii) recovering a fraction of the isolated genomic DNA having a desired characteristic; and (d) normalizing the representation of various DNAs within the genomic DNA population so as to form a normalized library of genomic DNA from the environmental sample.
Claim 2
Dependent Claim
104 words
A process for forming a normalized genomic DNA library from an environmental sample, which comprises the steps of: (a) isolating a genomic DNA population from the environmental sample; (b) analyzing the complexity of the genomic DNA population so isolated; (c) at least one of the steps selected from the group consisting of (i) amplifying the copy number of the DNA population so isolated and (ii) recovering a fraction of the isolated genomic DNA having a desired characteristic; and (d) normalizing the representation of various DNAs within the genomic DNA population so as to form a normalized library of genomic DNA from the environmental sample.
Claim 3
Dependent Claim
22 words
The process of claim 1 wherein step (c) consists of recovering a fraction of the isolated genomic DNA having a desired characteristic.
Claim 4
Dependent Claim
20 words
The process of claim 1 wherein step (c) consists of amplifying the copy number of the DNA population so isolated.
Claim 5
Dependent Claim
17 words
The process of claim 1 wherein the step of amplifying the genomic DNA precedes the normalizing step.
Claim 6
Dependent Claim
17 words
The process of claim 1 wherein the step of normalizing the genomic DNA precedes the amplifying step.
Claim 7
Dependent Claim
36 words
The process of claim 1 which comprises both the steps of (i) amplifying the copy number of the DNA population so isolated and (ii) recovering a fraction of the isolated genomic DNA having a desired characteristic.
Claim 8
Dependent Claim
96 words
A normalized genomic DNA library formed from from an environmental sample by a process comprising the steps of: (a) isolating a genomic DNA population from the environmental sample; (b) analyzing the complexity of the genomic DNA population so isolated; (c) at least one of (i) amplifying the copy number of the DNA population so isolated and (ii) recovering a fraction of the isolated genomic DNA having a desired characteristic; and (d) normalizing the representation of various DNAs within the genomic DNA population so as to form a normalized library of genomic DNA from the environmental sample.
Claim 9
Dependent Claim
96 words
A normalized genomic DNA library formed from from an environmental sample by a process comprising the steps of: (a) isolating a genomic DNA population from the environmental sample; (b) analyzing the complexity of the genomic DNA population so isolated; (c) at least one of (i) amplifying the copy number of the DNA population so isolated and (ii) recovering a fraction of the isolated genomic DNA having a desired characteristic; and (d) normalizing the representation of various DNAs within the genomic DNA population so as to form a normalized library of genomic DNA from the environmental sample.
Claim 10
Dependent Claim
25 words
The library of claim 7 wherein step (c) consists of the step of recovering a fraction of the isolated genomic DNA having a desired characteristic.
Claim 11
Dependent Claim
23 words
The library of claim 7 wherein step (c) consists of the step of amplifying the copy number of the DNA population so isolated.
Claim 12
Dependent Claim
24 words
The library of claim 7 wherein in the process of forming said library the step of amplifying the genomic DNA precedes the normalizing step.
Claim 13
Dependent Claim
24 words
The library of claim 7 wherein in the process of forming said library the step of normalizing the genomic DNA precedes the amplifying step.
Claim 14
Dependent Claim
42 words
The library of claim 7 wherein the process of forming said library comprises both the steps of (I) amplifying the copy number of the DNA population so isolated and (ii) recovering a fraction of the isolated genomic DNA having a desired characteristic.
Claim 15
Dependent Claim
95 words
A process for forming a normalized library of genomic gene clusters from an environmental sample which comprises (a) isolating a genomic DNA population from the environmental sample; (b) analyzing the complexity of the genomic DNA population so isolated; (c) at least one of (i) amplifying the copy number of the DNA population so isolated and (ii) recovering a fraction of the isolated genomic DNA having a desired characteristic; and (d) normalizing the representation of various DNAs within the genomic DNA population so as to form a normalized library of genomic DNA from the environmental sample.
Claim 16
Dependent Claim
95 words
A process for forming a normalized library of genomic gene clusters from an environmental sample which comprises (a) isolating a genomic DNA population from the environmental sample; (b) analyzing the complexity of the genomic DNA population so isolated; (c) at least one of (i) amplifying the copy number of the DNA population so isolated and (ii) recovering a fraction of the isolated genomic DNA having a desired characteristic; and (d) normalizing the representation of various DNAs within the genomic DNA population so as to form a normalized library of genomic DNA from the environmental sample.
Claim 17
Dependent Claim
97 words
A normalized library of genomic gene clusters formed from an environmental sample by a process comprising the steps of (a) isolating a genomic DNA population from the environmental sample; (b) analyzing the complexity of the genomic DNA population so isolated; (c) at least one of (I) amplifying the copy number of the DNA population so isolated and (ii) recovering a fraction of the isolated genomic DNA having a desired characteristic; and (d) normalizing the representation of various DNAs within the genomic DNA population so as to form a normalized library of genomic DNA from the environmental sample.
Claim 18
Dependent Claim
97 words
A normalized library of genomic gene clusters formed from an environmental sample by a process comprising the steps of (a) isolating a genomic DNA population from the environmental sample; (b) analyzing the complexity of the genomic DNA population so isolated; (c) at least one of (I) amplifying the copy number of the DNA population so isolated and (ii) recovering a fraction of the isolated genomic DNA having a desired characteristic; and (d) normalizing the representation of various DNAs within the genomic DNA population so as to form a normalized library of genomic DNA from the environmental sample.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2001-06-08 | 2001-12-08 | 2002-06-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2005-06-08 | 2005-12-08 | 2006-06-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2009-06-08 | 2009-12-08 | 2010-06-09 | $7700.00 | Window Closed / Lapsed |