US5750338A
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Target and background capture methods with amplification for affinity assays
Assignee: Abbott Molecular Inc
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Filing: 1994-05-03
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Granted: 1998-05-12
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Expires: 2014-05-03
Abstract
Abstract A method of assay for target polynucleotides includes steps of isolating target polynucleotides from extraneous non-target polynucleotides, debris, and impurities and amplifying the target polynucleotide.
Inventors
- 👤 Mark L. Collins
- 👤 Donald N. Halbert
- 👤 Walter King
- 👤 Jonathan M. Lawrie
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 10:24
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/10; C12N15/1003; C12N15/1006; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6844; C12Q1/686; G; G01; G01N; G01N35/00; G01N35/0098
Claims Analysis
Total Claims: 49
Claim 1
Independent Claim
47 words
A method for amplifying a target polynucleotide contained in a sample comprising the steps of: (a) contacting the sample with a first support which binds to the target polynucleotide; (b) substantially separating the support and bound target polynucleotide from the sample; and (c) amplifying the target polynucleotide.
Claim 2
Independent Claim
47 words
A method for amplifying a target polynucleotide contained in a sample comprising the steps of: (a) contacting the sample with a first support which binds to the target polynucleotide; (b) substantially separating the support and bound target polynucleotide from the sample; and (c) amplifying the target polynucleotide.
Claim 3
Dependent Claim
11 words
The method of claim 1 wherein the first support is retrievable.
Claim 4
Dependent Claim
18 words
The method of claim 1 wherein the first support includes a probe which binds with the target polynucleotide.
Claim 5
Dependent Claim
14 words
The method of claim 1 wherein the target polynucleotide is amplified with a polymerase.
Claim 6
Dependent Claim
20 words
The method of claim 4 wherein the polymerase is a DNA polymerase, an RNA polymerase, a transcriptase or Qβ replicase.
Claim 7
Dependent Claim
20 words
The method of claim 4 wherein the target polynucleotide is a DNA polynucleotide and the polymerase is a DNA polymerase.
Claim 8
Independent Claim
57 words
A method for detecting a target polynucleotide contained in a sample comprising the steps of: (a) contacting the sample with a first support which binds to the target polynucleotide; (b) substantially separating the first support and bound target polynucleotide from the sample; (c) amplifying the target polynucleotide; and (d) detecting the presence of the amplified target polynucleotide.
Claim 9
Independent Claim
57 words
A method for detecting a target polynucleotide contained in a sample comprising the steps of: (a) contacting the sample with a first support which binds to the target polynucleotide; (b) substantially separating the first support and bound target polynucleotide from the sample; (c) amplifying the target polynucleotide; and (d) detecting the presence of the amplified target polynucleotide.
Claim 10
Dependent Claim
11 words
The method of claim 7 wherein the first support is retrievable.
Claim 11
Dependent Claim
18 words
The method of claim 8 wherein the first support includes a probe which binds with the target polynucleotide.
Claim 12
Dependent Claim
14 words
The method of claim 7 wherein the target polynucleotide is amplified with a polymerase.
Claim 13
Dependent Claim
20 words
The method of claim 10 wherein the polymerase is a DNA polymerase, an RNA polymerase, a transcriptase or Qβ replicase.
Claim 14
Dependent Claim
20 words
The method of claim 11 wherein the target polynucleotide is a DNA polynucleotide and the polymerase is a DNA polymerase.
Claim 15
Dependent Claim
15 words
The method of claim 7 wherein the amplified target polynucleotide is contacted with a label.
Claim 16
Dependent Claim
16 words
The method of claim 7 wherein the amplified target polynucleotide is contacted with a labeled probe.
Claim 17
Dependent Claim
23 words
The method of claim 7 wherein the amplified target polynucleotide is contacted with a second support which binds to the amplified target polynucleotide.
Claim 18
Dependent Claim
16 words
The method of claim 15 wherein the amplified target polynucleotide is contacted with a labeled probe.
Claim 19
Dependent Claim
14 words
The method of claim 16 wherein the target polynucleotide is amplified with a polymerase.
Claim 20
Dependent Claim
20 words
The method of claim 17 wherein the target polynucleotide is a DNA polynucleotide and the polymerase is a DNA polymerase.
Claim 21
Independent Claim
90 words
A method for detecting a target polynucleotide contained in a sample comprising the steps of: (a) contacting the sample with a first support which binds to the target polynucleotide; (b) substantially separating the first support and bound target polynucleotide from the sample; (c) amplifying the sample with a DNA polymerase; (d) contacting the amplified target polynucleotide with a second support which binds to the amplified target polynucleotide and also with a labeled probe which binds to the amplified target polynucletide; and (e) detecting the presence of the amplified target polynucleotide.
Claim 22
Independent Claim
90 words
A method for detecting a target polynucleotide contained in a sample comprising the steps of: (a) contacting the sample with a first support which binds to the target polynucleotide; (b) substantially separating the first support and bound target polynucleotide from the sample; (c) amplifying the sample with a DNA polymerase; (d) contacting the amplified target polynucleotide with a second support which binds to the amplified target polynucleotide and also with a labeled probe which binds to the amplified target polynucletide; and (e) detecting the presence of the amplified target polynucleotide.
Claim 23
Dependent Claim
51 words
A kit for detecting a target polynucleotide contained in a sample comprising: (a) means for substantially separating the target polynucleotide from the sample; (b) means for amplifying the target polynucleotide; (c) means for binding the amplified target polynucleotide to a solid support; and (d) means for labeling the amplified target polynucleotide.
Claim 24
Dependent Claim
51 words
A kit for detecting a target polynucleotide contained in a sample comprising: (a) means for substantially separating the target polynucleotide from the sample; (b) means for amplifying the target polynucleotide; (c) means for binding the amplified target polynucleotide to a solid support; and (d) means for labeling the amplified target polynucleotide.
Claim 25
Dependent Claim
73 words
The kit of claim 20 wherein: (a) the means for substantially separating the target polynucleotide from the sample include a first support; (b) the means for amplifying the target polynucleotide include a polymerase; (c) the means for binding that amplified target polynucleotide to a solid support include a capture probe which binds to the solid support and to the amplified target polynucleotide; and (d) a detector probe for labeling the amplified target polynucleotide.
Claim 26
Dependent Claim
20 words
The kit of claim 21 further comprising a capture probe which binds to the first support and to the target.
Claim 27
Dependent Claim
18 words
The kit of claim 22 wherein the polymerase is a DNA polymerase and the detector probe is labeled.
Claim 28
Dependent Claim
31 words
A kit for amplifying a target polynucleotide contained in a sample comprising: (a) means for substantially separating the target polynucleotide from the sample and (b) means for amplifying the target polynucleotide.
Claim 29
Dependent Claim
31 words
A kit for amplifying a target polynucleotide contained in a sample comprising: (a) means for substantially separating the target polynucleotide from the sample and (b) means for amplifying the target polynucleotide.
Claim 30
Dependent Claim
39 words
The kit of claim 24 wherein: (a) the means for substantially separating the target polynucleotide from the sample includes a support which binds to the target polynucleotide and (b) the means for amplifying the target polynucleotide includes a polymerase.
Claim 31
Dependent Claim
38 words
The kit of claim 25 wherein: (a) the polymerase is a DNA polymerase; and (b) the means for substantially separating the target polynucleotide from the sample includes a probe which binds to the target polynucleotide and the support.
Claim 32
Independent Claim
108 words
A method for amplifying a target polynucleotide contained in a sample medium comprising the steps of: (a) contacting the sample medium with reagent comprising a first nucleic acid probe which binds to the target to form a probe-target complex; (b) contacting the sample medium with a support which binds to the first nucleic acid probe of the probe-target complex; (c) substantially separating the support and bound probe-target complex from the sample medium; (d) contacting the support and bound probe-target complex with a second medium; (e) releasing the probe-target complex into the second medium; (f) substantially separating the support from the second medium; and (g) amplifying the target polynucleotide.
Claim 33
Independent Claim
108 words
A method for amplifying a target polynucleotide contained in a sample medium comprising the steps of: (a) contacting the sample medium with reagent comprising a first nucleic acid probe which binds to the target to form a probe-target complex; (b) contacting the sample medium with a support which binds to the first nucleic acid probe of the probe-target complex; (c) substantially separating the support and bound probe-target complex from the sample medium; (d) contacting the support and bound probe-target complex with a second medium; (e) releasing the probe-target complex into the second medium; (f) substantially separating the support from the second medium; and (g) amplifying the target polynucleotide.
Claim 34
Independent Claim
116 words
A method for detecting a target polynucleotide contained in a sample medium comprising the steps of: (a) contacting the sample medium with reagent comprising a first nucleic acid probe which binds to the target to form a probe-target complex; (b) contacting the sample medium with a support which binds to the first nucleic acid probe of the probe-target complex; (c) substantially separating the support and bound probe-target complex from the sample medium; (d) contacting the support and bound probe-target complex with a second medium; (e) releasing the probe-target complex into the second medium; (f) substantially separating the support from the second medium; (g) amplifying the target polynucleotide; and (h) detecting the presence of the target polynucleotide.
Claim 35
Independent Claim
116 words
A method for detecting a target polynucleotide contained in a sample medium comprising the steps of: (a) contacting the sample medium with reagent comprising a first nucleic acid probe which binds to the target to form a probe-target complex; (b) contacting the sample medium with a support which binds to the first nucleic acid probe of the probe-target complex; (c) substantially separating the support and bound probe-target complex from the sample medium; (d) contacting the support and bound probe-target complex with a second medium; (e) releasing the probe-target complex into the second medium; (f) substantially separating the support from the second medium; (g) amplifying the target polynucleotide; and (h) detecting the presence of the target polynucleotide.
Claim 36
Dependent Claim
19 words
The method of detecting a target polynucleotide of claim 28 wherein the target polynucleotide is amplified with a polymerase.
Claim 37
Dependent Claim
25 words
The method for detecting a target polynucleotide of claim 29 wherein the polymerase is a DNA polymerase, an RNA polymerase, a transcriptase, or Qβ replicase.
Claim 38
Dependent Claim
17 words
The method for detecting a target polynucleotide of claim 30 wherein the polymerase is a DNA polymerase.
Claim 39
Dependent Claim
19 words
The method for amplifying a target polynucleotide of claim 27 wherein the target polynucleotide is amplified with a polymerase.
Claim 40
Dependent Claim
17 words
The method for amplifying a target polynucleotide of claim 32 wherein the polymerase is a DNA polymerase.
Claim 41
Independent Claim
92 words
A method for amplifying a target polynucleotide contained in a sample medium comprising the steps of: (a) contacting the sample medium with a support and a probe which binds to the target polynucleotide and the support; (b) substantially separating the support and bound probe and target polynucleotide from the sample medium; (c) contacting the support and bound probe and target polynucleotide with a second medium; (d) releasing the target polynucleotide into the second medium; (e) substantially separating the support and bound probe from the second medium; and (f) amplifying the target polynucleotide.
Claim 42
Independent Claim
92 words
A method for amplifying a target polynucleotide contained in a sample medium comprising the steps of: (a) contacting the sample medium with a support and a probe which binds to the target polynucleotide and the support; (b) substantially separating the support and bound probe and target polynucleotide from the sample medium; (c) contacting the support and bound probe and target polynucleotide with a second medium; (d) releasing the target polynucleotide into the second medium; (e) substantially separating the support and bound probe from the second medium; and (f) amplifying the target polynucleotide.
Claim 43
Dependent Claim
18 words
The method for amplifying a target polynucleotide of claim 34 wherein the target polynucleotide is amplified a polymerase.
Claim 44
Dependent Claim
25 words
The method for amplifying a target polynucleotide of claim 35 wherein the polymerase is a DNA polymerase, an RNA polymerase, a transcriptase or Qβ replicase.
Claim 45
Dependent Claim
17 words
The method for amplifying a target polynucleotide of claim 36 wherein the polymerase is a DNA polymerase.
Claim 46
Independent Claim
100 words
A method for detecting a target polynucleotide contained in a sample medium comprising the steps of: (a) contacting the sample medium with a support and probe which binds to the target polynucleotide and the support; (b) substantially separating the support and bound probe and target polynucleotide from the sample medium; (c) contacting the support and bound probe and target polynucleotide with a second medium; (d) releasing the target polynucleotide into the second medium; (e) substantially separating the support and bound probe form the second medium; (f) amplifying the target polynucleotide; and (g) detecting the presence of the amplified target polynucleotide.
Claim 47
Independent Claim
100 words
A method for detecting a target polynucleotide contained in a sample medium comprising the steps of: (a) contacting the sample medium with a support and probe which binds to the target polynucleotide and the support; (b) substantially separating the support and bound probe and target polynucleotide from the sample medium; (c) contacting the support and bound probe and target polynucleotide with a second medium; (d) releasing the target polynucleotide into the second medium; (e) substantially separating the support and bound probe form the second medium; (f) amplifying the target polynucleotide; and (g) detecting the presence of the amplified target polynucleotide.
Claim 48
Dependent Claim
19 words
The method for detecting a target polynucleotide of claim 38 wherein the target polynucleotide is amplified with a polymerase.
Claim 49
Dependent Claim
17 words
The method for detecting a target polynucleotide of claim 39 wherein the polymerase is a DNA polymerase.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2001-05-11 | 2001-11-10 | 2002-05-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2005-05-11 | 2005-11-10 | 2006-05-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2009-05-11 | 2009-11-10 | 2010-05-12 | $7700.00 | Window Closed / Lapsed |