US5744350A
Expired / Lapsed
US
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DNA encoding triol polyketide synthase
Assignee: Merck and Co Inc
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Filing: 1995-05-25
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Granted: 1998-04-28
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Expires: 2015-05-25
Abstract
Abstract DNA encoding triol polyketide synthase (TPKS) has been isolated, purified and sequenced. Expression vectors comprising TPKS, cells transformed with the expression vectors, and processes employing the transformed cells are provided.
Inventors
- 👤 Victor A. Vinci
- 👤 Michael J. Conder
- 👤 Phyllis C. McAda
- 👤 Christopher D. Reeves
- 👤 John Rambosek
- 👤 Charles Ray Davis
- 👤 Lee E. Hendrickson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 00:37
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/88; C12P; C12P17/00; C12P17/02; C12P17/06
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
20 words
Purified DNA encoding triol polyketide synthase from a microorganism selected from the group consisting of Monascus ruber and Penicillum citrinum.
Claim 2
Dependent Claim
20 words
Purified DNA encoding triol polyketide synthase from a microorganism selected from the group consisting of Monascus ruber and Penicillum citrinum.
Claim 3
Dependent Claim
10 words
An expression vector comprising the DNA molecule of claim 1.
Claim 4
Dependent Claim
11 words
A host cell transformed with the expression vector of claim 2.
Claim 5
Dependent Claim
41 words
A process for producing HMG-CoA reductase inhibitors, comprising: (a) transforming a cell with the DNA molecule of claim 1; (b) cultivating the transformed cell under conditions that permit the expression of the DNA molecule; and (c) recovering the HMG-CoA reductase inhibitor.
Claim 6
Dependent Claim
21 words
The process of claim 4 wherein the HMG-CoA reductase inhibitors are selected from the group consisting of lovastatin, triol and compactin.
Claim 7
Dependent Claim
42 words
The process of claim 5 wherein and transformed cell is selected from the group consisting of Aspergillus terreus, Monascus ruber, Penicillum citrinum, Penicillum brevicompactum, Hypomyces chrysospermus, Paecilomyces viridis L-63 Paecilomyces sp. M2016, Eupenicillium sp. MM603, Trichoderma longibrachiatum M6735 and Trichoderma pseudokoningii M6828.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2001-04-27 | 2001-10-27 | 2002-04-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2005-04-27 | 2005-10-27 | 2006-04-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2009-04-27 | 2009-10-27 | 2010-04-28 | $7700.00 | Window Closed / Lapsed |