US5714476A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for preparing compositions containing neotrehalose
Assignee: Hayashibara Seibutsu Kagaku Kenkyujo KK
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Filing: 1994-09-30
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Granted: 1998-02-03
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Expires: 2014-09-30
Abstract
Abstract Neotrehalose is prepared in a relatively high-yield by a process comprising allowing β-galactosidase to act on a solution containing lactoneotrehalose to form neotrehalose and recoverying the resultant neotrehalose. The neotrehalose is a non-reducing oligosaccharide having a satisfiable stability and a rich- and high-quality-sweetness and is assimilated and utilized as energy source in vivo when orally administered. Neotrehalose in the form of crystal has a satisfiable handleability because it is readily soluble in water and substantially free of hygroscopicity. These render neotrehalose very useful in the fields of food-, cosmetic- and pharmaceutical-industries.
Inventors
- 👤 Yoshikatsu Miwa
- 👤 Takashi Shibuya
- 👤 Hiroto Chaen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 07:03
CPC Classifications
C; C07; C07H; C07H3/00; C07H3/04; C12; C12P; C12P19/00; C12P19/18; C12P19/14
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
48 words
A process for preparing an orally-administrable composition comprising neotrehalose and an orally-administrable carrier, which comprises: (a) treating a solution containing lactoneotrehalose of the formula O-β-D-galactopyranosyl (1-4)-O-β-D-glycopyranosyl α-D-glucopyranoside with β-D-galactosidase (EC 3.2.1.23) to form neotrehalose; (b) recovering the resulting neotrehalose; and (c) incorporating the neotrehalose into an orally-administrable carrier.
Claim 2
Dependent Claim
48 words
A process for preparing an orally-administrable composition comprising neotrehalose and an orally-administrable carrier, which comprises: (a) treating a solution containing lactoneotrehalose of the formula O-β-D-galactopyranosyl (1-4)-O-β-D-glycopyranosyl α-D-glucopyranoside with β-D-galactosidase (EC 3.2.1.23) to form neotrehalose; (b) recovering the resulting neotrehalose; and (c) incorporating the neotrehalose into an orally-administrable carrier.
Claim 3
Dependent Claim
23 words
The process according to claim 1 wherein said orally-administrable carrier is selected from the group consisting of foods, cigarettes, animal feed and pharmaceuticals.
Claim 4
Dependent Claim
23 words
The process according to claim 1 wherein step (c) includes a step of incorporating at least one additional sweetener into said orally-administrable carrier.
Claim 5
Dependent Claim
26 words
The process according to claim 1 wherein said neotrehalose is formulated with at least one filler selected from the group consisting of dextrin, starch and lactose.
Claim 6
Dependent Claim
46 words
A process for preparing a cosmetic comprising neotrehalose and a cosmetically-acceptable carrier, which comprises: (a) treating a solution containing lactoneotrehalose of the formula O-β-D-galactopyranosyl(1→4)-O-β-D-glucopyranosyl α-D-glucopyranoside with β-D-galactosidase (EC 3.2.1.23) to form neotrehalose; (b) recovering the resulting neotrehalose; and (c) incorporating the neotrehalose into a cosmetically-acceptable carrier.
Claim 7
Dependent Claim
46 words
A process for preparing a cosmetic comprising neotrehalose and a cosmetically-acceptable carrier, which comprises: (a) treating a solution containing lactoneotrehalose of the formula O-β-D-galactopyranosyl(1→4)-O-β-D-glucopyranosyl α-D-glucopyranoside with β-D-galactosidase (EC 3.2.1.23) to form neotrehalose; (b) recovering the resulting neotrehalose; and (c) incorporating the neotrehalose into a cosmetically-acceptable carrier.
Claim 8
Dependent Claim
25 words
The process according to claim 5 wherein the cosmetically-acceptable carrier is selected from the group consisting of dentifrices, lipstick, lip cream, oral rinses, and gargles.
Claim 9
Dependent Claim
52 words
A process for preparing a pharmaceutical composition comprising neotrehalose and a pharmaceutically-effective ingredient which comprises: (a) treating a solution containing lactoneotrehalose of the formula O-β-D-galactopyranosyl (1→4)-O-β-D-glucopyranosyl α-D-glucopyranoside with β-D-galactosidase (EC 3.2.1.23) to form neotrehalose; (b) recovering the resulting neotrehalose; and (c) incorporating the neotrehalose into a composition comprising a pharmaceutically effective ingredient.
Claim 10
Dependent Claim
52 words
A process for preparing a pharmaceutical composition comprising neotrehalose and a pharmaceutically-effective ingredient which comprises: (a) treating a solution containing lactoneotrehalose of the formula O-β-D-galactopyranosyl (1→4)-O-β-D-glucopyranosyl α-D-glucopyranoside with β-D-galactosidase (EC 3.2.1.23) to form neotrehalose; (b) recovering the resulting neotrehalose; and (c) incorporating the neotrehalose into a composition comprising a pharmaceutically effective ingredient.
Claim 11
Dependent Claim
25 words
The process according to claim 7 wherein step (c) includes a step of incorporating a pharmaceutically-acceptable carrier into said composition comprising a pharmaceutically effective ingredient.
Claim 12
Dependent Claim
66 words
A process for preparing an orally-administrable product comprising neotrehalose and an orally-administrable carrier, which comprises: (a) treating a solution containing lactotrehalose of the formula O-β-D-galactopyranosyl (1→4)-O-β-D-glucopyranosyl α-D-glucopyranoside with β-D-galactosidase (EC 3.2.1.23) to form neotrehalose; (b) purifying and concentrating the resultant solution into a supersaturated solution of neotrehalose to crystallize neotrehalose; (c) recovering the resultant crystalline neotrehalose; and (d) incorporating the crystalline neotrehalose into an orally-administrable carrier.
Claim 13
Dependent Claim
66 words
A process for preparing an orally-administrable product comprising neotrehalose and an orally-administrable carrier, which comprises: (a) treating a solution containing lactotrehalose of the formula O-β-D-galactopyranosyl (1→4)-O-β-D-glucopyranosyl α-D-glucopyranoside with β-D-galactosidase (EC 3.2.1.23) to form neotrehalose; (b) purifying and concentrating the resultant solution into a supersaturated solution of neotrehalose to crystallize neotrehalose; (c) recovering the resultant crystalline neotrehalose; and (d) incorporating the crystalline neotrehalose into an orally-administrable carrier.
Claim 14
Dependent Claim
23 words
The process according to claim 9 wherein said orally-administrable carrier is selected from the group consisting of foods, cigarettes, animal feeds, and pharmaceuticals.
Claim 15
Dependent Claim
23 words
The process according to claim 9 wherein step (d) includes a step of incorporating at least one additional sweetener into said orally-administrable carrier.
Claim 16
Dependent Claim
32 words
The process according to claim 9 wherein step (d) includes a step of incorporating at least one filler selected from the group consisting of dextrin, starch and lactose into said orally-administrable carrier.
Claim 17
Dependent Claim
48 words
A process for preparing a cosmetic composition comprising neotrehalose and a cosmetically-acceptable carrier which comprises: (a) treating a solution containing lactoneotrehalose of the formula O-β-D-galactopyranosyl (1→4)-O-β-D-glucopyranosyl α-D-glucopyranoside with β-D-galactosidase (EC 3.2.1.23) to form neotrehalose; (b) recovering the resulting neotrehalose; and (c) incorporating the neotrehalose into a cosmetically-acceptable carrier.
Claim 18
Dependent Claim
48 words
A process for preparing a cosmetic composition comprising neotrehalose and a cosmetically-acceptable carrier which comprises: (a) treating a solution containing lactoneotrehalose of the formula O-β-D-galactopyranosyl (1→4)-O-β-D-glucopyranosyl α-D-glucopyranoside with β-D-galactosidase (EC 3.2.1.23) to form neotrehalose; (b) recovering the resulting neotrehalose; and (c) incorporating the neotrehalose into a cosmetically-acceptable carrier.
Claim 19
Dependent Claim
25 words
The process according to claim 13 wherein said cosmetically-acceptable carrier is selected from the group consisting of dentifrices, lipstick, lip cream, oral rinses, and gargles.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 2001-02-02 | 2001-08-04 | 2002-02-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2005-02-02 | 2005-08-04 | 2006-02-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2009-02-02 | 2009-08-04 | 2010-02-03 | $7700.00 | Window Closed / Lapsed |