US5712146A
Expired / Lapsed
US
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Recombinant combinatorial genetic library for the production of novel polyketides
Assignee: John Innes Centre
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Filing: 1995-06-07
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Granted: 1998-01-27
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Expires: 2015-06-07
Abstract
Abstract Novel polyketides and novel methods of efficiently producing both new and known polyketides, using recombinant technology, are disclosed. In particular, a novel host-vector system is described which is used to produce polyketide synthases which in turn catalyze the production of a variety of polyketides.
Inventors
- 👤 Chaitan Khosla
- 👤 David A. Hopwood
- 👤 Suzanne Ebert-Khosla
- 👤 Robert McDaniel
- 👤 Hong Fu
- 👤 Camilla Kao
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 00:28
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/93; C07; C07D; C07D309/00; C07D309/34; C07D309/36; C07D311/00; C07D311/02; C07D311/78; C07D311/92; C07D323/00; C07D407/00; C07D407/02; C07D407/06; C07H; C07H17/00; C07H17/04; C07H17/08; C12N15/00; C12N15/09; C12N15/11; C12N15/52; C12P; C12P17/00; C12P17/02; C12P17/06; C12P17/08; C12P17/16; C12P17/162; C12P7/00; C12P7/24; C12P7/26; C40; C40B; C40B40/00
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
91 words
A library for synthesis of a multiplicity of polyketides which library comprises a multiplicity of individual cell lines wherein each cell line produces a functional polyketide synthase (PKS) such that each said cell line produces a different polyketide; wherein each functional PKS comprises the products of: a first open reading frame (ORF) encoding a ketosynthase/acyltransferase (KS/AT); a second ORF encoding an acyl carrier protein (ACP); and a third ORF encoding a chain length determining factor (CLDF); wherein the ORFs in said library are derived from at least three different aromatic PKS.
Claim 2
Dependent Claim
91 words
A library for synthesis of a multiplicity of polyketides which library comprises a multiplicity of individual cell lines wherein each cell line produces a functional polyketide synthase (PKS) such that each said cell line produces a different polyketide; wherein each functional PKS comprises the products of: a first open reading frame (ORF) encoding a ketosynthase/acyltransferase (KS/AT); a second ORF encoding an acyl carrier protein (ACP); and a third ORF encoding a chain length determining factor (CLDF); wherein the ORFs in said library are derived from at least three different aromatic PKS.
Claim 3
Dependent Claim
39 words
The library of claim 1 wherein at least some of said multiplicity of cell lines further include an ORF encoding a polyketide cyclase (CYC); and/or an ORF encoding polyketide aromatase (ARO); and/or an ORF encoding a polyketide ketoreductase (KR).
Claim 4
Dependent Claim
65 words
The library of claim 1 which includes colonies wherein the functional PKS comprises the product of at least one of: ______________________________________
ORF1 ORF2 ORF3
(KS/AT) (CLDF) (ACP)
______________________________________
act act act
gra act act
act gra act
act act gra
tcm act act
tcm tcm act
act act tcm
fren act act
act act fren
tcm tcm tcm
fren fren act
fren fren fren
______________________________________
Claim 5
Dependent Claim
33 words
The library of claim 1 wherein said cell line further comprises an enzyme that is active on a PKS and is selected from the group consisting of an O-methyl transferase and glycosyl transferase.
Claim 6
Dependent Claim
26 words
The library of claim 1 wherein in at least one member of said library each said open reading frame is contained in a separate expression cassette.
References Cited (Backward Citations)
US4935340A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2001-01-26 | 2001-07-28 | 2002-01-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2005-01-26 | 2005-07-28 | 2006-01-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2009-01-26 | 2009-07-28 | 2010-01-27 | $7700.00 | Window Closed / Lapsed |