US5688648A
Expired / Lapsed
US
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Probes labelled with energy transfer coupled dyes
Assignee: University of California, San Diego
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Filing: 1995-12-19
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Granted: 1997-11-18
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Expires: 2015-12-19
Abstract
Abstract Compositions are provided comprising sets of fluorescent labels carrying pairs of donor and acceptor dye molecules, designed for efficient excitation of the donors at a single wavelength and emission from the acceptor in each of the pairs at different wavelengths. The different molecules having different donor-acceptor pairs can be modified to have substantially the same mobility under separation conditions, by varying the distance between the donor and acceptor in a given pair. Particularly, the fluorescent compositions find use as labels in sequencing nucleic acids.
Inventors
- 👤 Richard A. Mathies
- 👤 Alexander Glazer
- 👤 Jingyue Ju
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 17:08
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/58; G01N33/582; C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6816; C12Q1/6818; G01N33/53; G01N33/536; G01N33/542; Y; Y10; Y10S; Y10S435/00; Y10S435/81
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
131 words
A method of identification and detection of nucleic acids in a multi-nucleic acid mixture employing detectably different fluorescent labels to detect at least two nucleic acids of interest, wherein said labels are characterized by: (1) having a donor-acceptor fluorescent pair where said donor and said acceptor are each covalently bonded to a backbone chain at specific locations thereon with energy transfer from said donor to said acceptor; and (2) each of the labels absorbs at substantially the same wavelength and emits at a different wavelength; said method comprising: covalently bonding different labels to different nucleic acids of said multi-nucleic acid mixture of form labeled nucleic acids; detecting each of said labeled nucleic acids by irradiating at the absorption wavelength of said donor and detecting the fluorescence of each of said labels.
Claim 2
Independent Claim
131 words
A method of identification and detection of nucleic acids in a multi-nucleic acid mixture employing detectably different fluorescent labels to detect at least two nucleic acids of interest, wherein said labels are characterized by: (1) having a donor-acceptor fluorescent pair where said donor and said acceptor are each covalently bonded to a backbone chain at specific locations thereon with energy transfer from said donor to said acceptor; and (2) each of the labels absorbs at substantially the same wavelength and emits at a different wavelength; said method comprising: covalently bonding different labels to different nucleic acids of said multi-nucleic acid mixture of form labeled nucleic acids; detecting each of said labeled nucleic acids by irradiating at the absorption wavelength of said donor and detecting the fluorescence of each of said labels.
Claim 3
Dependent Claim
30 words
A method according to claim 1, wherein said donor absorbs light in the wavelength range of 350-800 nm and said acceptor emits light in the wavelength range of 450-1000 nm.
Claim 4
Dependent Claim
12 words
A method according to claim 2, wherein said donor-acceptor pair are 9-phenylxanthenes.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2000-11-17 | 2001-05-19 | 2001-11-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2004-11-17 | 2005-05-19 | 2005-11-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2008-11-17 | 2009-05-19 | 2009-11-18 | $7700.00 | Window Closed / Lapsed |