US5660982A
Expired / Lapsed
US
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Laminin chains: diagnostic uses
Assignee: BS BIOTEKNIK AB
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Filing: 1994-10-04
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Granted: 1997-08-26
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Expires: 2014-10-04
Abstract
Abstract The instant invention provides for the identification, diagnosis, monitoring, and treatment of invasive cells using the laminin 5 gamma-2 chain protein or nucleic acid sequence, or antibodies thereto.
Inventors
- 👤 Karl Tryggvason
- 👤 Pekka Kallunki
- 👤 Charles Pyke
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 13:50
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/18; C07K16/28; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6876; C12Q1/6883; C12Q1/6886; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/575; G01N33/5751; G01N33/57535; G01N33/5755; G01N33/5758; C12Q2600/00; C12Q2600/158; G01N2333/00; G01N2333/435; G01N2333/78; G01N2474/00; G01N2474/20; Y; Y10; Y10S; Y10S436/00; Y10S436/811; Y10S436/813
Claims Analysis
Total Claims: 33
Claim 1
Independent Claim
103 words
A method for detecting kalinin/laminin 5 expression in cells and tissue comprising: a) hybridizing the cells and tissue with an effective amount of a nucleic acid probe, said probe consisting of a sense or antisense portion of a kalinin/laminin 5 gamma-2 nucleic acid sequence as shown in FIG. 4A wherein said probe specifically hybridizes to kalinin/laminin 5 gamma-2 chain nucleic acids in said cells and tissue and wherein a signal results from said hybridizing in cells and tissue expressing kalinin/laminin 5; b) detecting said signal whereby the presence of said signal is an indication of kalinin/laminin 5 expression in said cells and tissue.
Claim 2
Independent Claim
103 words
A method for detecting kalinin/laminin 5 expression in cells and tissue comprising: a) hybridizing the cells and tissue with an effective amount of a nucleic acid probe, said probe consisting of a sense or antisense portion of a kalinin/laminin 5 gamma-2 nucleic acid sequence as shown in FIG. 4A wherein said probe specifically hybridizes to kalinin/laminin 5 gamma-2 chain nucleic acids in said cells and tissue and wherein a signal results from said hybridizing in cells and tissue expressing kalinin/laminin 5; b) detecting said signal whereby the presence of said signal is an indication of kalinin/laminin 5 expression in said cells and tissue.
Claim 3
Dependent Claim
12 words
The method of claim 1 wherein the nucleic acid probe is DNA.
Claim 4
Dependent Claim
12 words
The method of claim 1 wherein the nucleic acid probe is RNA.
Claim 5
Dependent Claim
20 words
The method of claim 1 wherein the nucleic acid probe is radiolabeled, enzyme labeled, chemiluminescent labeled, avidin or biotin labeled.
Claim 6
Dependent Claim
35 words
The method of claim 1 wherein the nucleic acid probe comprises a fragment of human kalinin/laminin 5 gamma-2 chain nucleic acid sequence and wherein said probe specifically hybridizes to kalinin/laminin 5 gamma-2 chain nucleic acids.
Claim 7
Dependent Claim
17 words
The method of claim 1 wherein the nucleic acid probe is incorporated into an extrachromosomal self-replicating vector.
Claim 8
Dependent Claim
16 words
The method of claim 1 wherein the nucleic acid probe is incorporated into a viral vector.
Claim 9
Dependent Claim
12 words
The method of claim 1 wherein the nucleic acid probe is linear.
Claim 10
Dependent Claim
12 words
The method of claim 1 wherein the nucleic acid probe is circularized.
Claim 11
Dependent Claim
13 words
The method of claim 1 wherein the nucleic acid probe contains modified nucleotides.
Claim 12
Independent Claim
92 words
A method for detecting the presence of invasive cells in tissue comprising: a) hybridizing the tissue with an effective amount of a nucleic acid probe, said probe consisting of a sense or antisense portion of a kalinin/laminin 5 gamma-2 nucleic acid sequence as shown in FIG. 4A wherein said probe specifically hybridizes to kalinin/laminin 5 gamma-2 chain nucleic acids in said invasive cells and wherein a signal results from said hybridizing; b) detecting said signal whereby the presence of said signal is an indication of the presence of invasive cells in tissue.
Claim 13
Independent Claim
92 words
A method for detecting the presence of invasive cells in tissue comprising: a) hybridizing the tissue with an effective amount of a nucleic acid probe, said probe consisting of a sense or antisense portion of a kalinin/laminin 5 gamma-2 nucleic acid sequence as shown in FIG. 4A wherein said probe specifically hybridizes to kalinin/laminin 5 gamma-2 chain nucleic acids in said invasive cells and wherein a signal results from said hybridizing; b) detecting said signal whereby the presence of said signal is an indication of the presence of invasive cells in tissue.
Claim 14
Dependent Claim
12 words
The method of claim 11 wherein the nucleic acid probe is DNA.
Claim 15
Dependent Claim
12 words
The method of claim 11 wherein the nucleic acid probe is RNA.
Claim 16
Dependent Claim
20 words
The method of claim 11 wherein the nucleic acid probe is radiolabeled, enzyme labeled, chemiluminescent labeled, avidin or biotin labeled.
Claim 17
Dependent Claim
35 words
The method of claim 11 wherein the nucleic acid probe comprises a fragment of human kalinin/laminin 5 gamma-2 chain nucleic acid sequence and wherein said probe specifically hybridizes to kalinin/laminin 5 gamma-2 chain nucleic acids.
Claim 18
Dependent Claim
17 words
The method of claim 11 wherein the nucleic acid probe is incorporated into an extrachromosomal self-replicating vector.
Claim 19
Dependent Claim
16 words
The method of claim 11 wherein the nucleic acid probe is incorporated into a viral vector.
Claim 20
Dependent Claim
12 words
The method of claim 11 wherein the nucleic acid probe is linear.
Claim 21
Dependent Claim
12 words
The method of claim 11 wherein the nucleic acid probe is circularized.
Claim 22
Dependent Claim
13 words
The method of claim 11 wherein the nucleic acid probe contains modified nucleotides.
Claim 23
Independent Claim
82 words
A method for monitoring the presence of invasive cells in tissue comprising: a) hybridizing the tissue with an effective amount of a nucleic acid probe, said probe consisting of a sense or antisense portion of a kalinin/laminin 5 gamma-2 nucleic acid sequence as shown in FIG. 4A wherein said probe specifically hybridizes to kalinin/laminin 5 gamma-2 chain nucleic acids in said invasive cells and wherein a signal results from said hybridizing; b) monitoring said signal thereby monitoring the presence of invasive cells.
Claim 24
Independent Claim
82 words
A method for monitoring the presence of invasive cells in tissue comprising: a) hybridizing the tissue with an effective amount of a nucleic acid probe, said probe consisting of a sense or antisense portion of a kalinin/laminin 5 gamma-2 nucleic acid sequence as shown in FIG. 4A wherein said probe specifically hybridizes to kalinin/laminin 5 gamma-2 chain nucleic acids in said invasive cells and wherein a signal results from said hybridizing; b) monitoring said signal thereby monitoring the presence of invasive cells.
Claim 25
Dependent Claim
12 words
The method of claim 21 wherein the nucleic acid probe is DNA.
Claim 26
Dependent Claim
12 words
The method of claim 21 wherein the nucleic acid probe is RNA.
Claim 27
Dependent Claim
20 words
The method of claim 21 wherein the nucleic acid probe is radiolabeled, enzyme labeled, chemiluminescent labeled, avidin or biotin labeled.
Claim 28
Dependent Claim
35 words
The method of claim 21 wherein the nucleic acid probe comprises a fragment of human kalinin/laminin 5 gamma-2 chain nucleic acid sequence and wherein said probe specifically hybridizes to kalinin/laminin 5 gamma-2 chain nucleic acids.
Claim 29
Dependent Claim
17 words
The method of claim 21 wherein the nucleic acid probe is incorporated into an extrachromosomal self-replicating vector.
Claim 30
Dependent Claim
16 words
The method of claim 21 wherein the nucleic acid probe is incorporated into a viral vector.
Claim 31
Dependent Claim
12 words
The method of claim 21 wherein the nucleic acid probe is linear.
Claim 32
Dependent Claim
12 words
The method of claim 21 wherein the nucleic acid probe is circularized.
Claim 33
Dependent Claim
13 words
The method of claim 21 wherein the nucleic acid probe contains modified nucleotides.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2000-08-25 | 2001-02-24 | 2001-08-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2004-08-25 | 2005-02-24 | 2005-08-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2008-08-25 | 2009-02-24 | 2009-08-26 | $7700.00 | Window Closed / Lapsed |