US5654419A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Fluorescent labels and their use in separations
Assignee: University of California
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Filing: 1994-02-01
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Granted: 1997-08-05
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Expires: 2014-02-01
Abstract
Abstract Compositions are provided comprising sets of fluorescent labels carrying pairs of donor and acceptor dye molecules, designed for efficient excitation of the donors at a single wavelength and emission from the acceptor in each of the pairs at different wavelengths. The different molecules having different donor-acceptor pairs can be modified to have substantially the same mobility under separation conditions, by varying the distance between the donor and acceptor in a given pair. Particularly, the fluorescent compositions find use as labels in sequencing nucleic acids.
Inventors
- 👤 Richard A. Mathies
- 👤 Alexander Glazer
- 👤 Jingyue Ju
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 05:44
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/58; G01N33/582; C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6816; C12Q1/6818; G01N33/53; G01N33/536; G01N33/542; Y; Y10; Y10S; Y10S435/00; Y10S435/81
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
214 words
A method of separating nucleic acid components of a nucleic acid multicomponent mixture, wherein each of the different nucleic acid components or combinations of components of interest are labeled with different labels comprising different fluorescer pairs, wherein said labels are characterized by: (1) having a donor-acceptor fluorescent pair bonded to an oligonucleotide chain with efficient energy transfer from said donor to said acceptor; (2) each of the donor members of the labels absorbs at substantially the same wavelength as all of the other donor members and each of the acceptor members of the labels emits at a different wavelength compared to any of the other acceptor members; and each of the different labels has substantially the same mobility in said separating as a result of varying the spacer of said donor-acceptor pair along said oligonucleotide chain, with the proviso that one of said labels can have the same fluorescer as the donor and the acceptor; said method comprising: binding different labels to different nucleic acid components or combinations of components of said multi-component mixture; separating said nucleic acid components into individual fractions; and detecting each of said labeled nucleic acid components by irradiating with a common light source at an absorption wavelength of said donors and detecting the fluorescence of each of said labels.
Claim 2
Independent Claim
214 words
A method of separating nucleic acid components of a nucleic acid multicomponent mixture, wherein each of the different nucleic acid components or combinations of components of interest are labeled with different labels comprising different fluorescer pairs, wherein said labels are characterized by: (1) having a donor-acceptor fluorescent pair bonded to an oligonucleotide chain with efficient energy transfer from said donor to said acceptor; (2) each of the donor members of the labels absorbs at substantially the same wavelength as all of the other donor members and each of the acceptor members of the labels emits at a different wavelength compared to any of the other acceptor members; and each of the different labels has substantially the same mobility in said separating as a result of varying the spacer of said donor-acceptor pair along said oligonucleotide chain, with the proviso that one of said labels can have the same fluorescer as the donor and the acceptor; said method comprising: binding different labels to different nucleic acid components or combinations of components of said multi-component mixture; separating said nucleic acid components into individual fractions; and detecting each of said labeled nucleic acid components by irradiating with a common light source at an absorption wavelength of said donors and detecting the fluorescence of each of said labels.
Claim 3
Dependent Claim
12 words
A method according to claim 1, wherein said separation is by electrophoresis.
Claim 4
Dependent Claim
66 words
A method according to claim 7, wherein each of said donors of each of said labels absorbs light in the wavelength range of 350-800 nm and each of said acceptors of each of said labels emits light in the wavelength range of 450-1000 nm, wherein when said acceptor is the same as said donor, both absorption and emission are in the wavelength range of 350-800 nm.
Claim 5
Dependent Claim
12 words
A method according to claim 3, wherein said acceptor--donor pair are 9-phenylxanthenes.
Claim 6
Dependent Claim
34 words
A method according to claim 1, wherein said binding comprises: combining said labels with said nucleic acid multicomponent mixture, wherein said labels act as primers; and extending said primers under polymerase chain reaction conditions.
Claim 7
Dependent Claim
135 words
A kit comprising at least two fluorescent compounds for use as labels, each of said labels characterized by: (1) comprising an acceptor-donor fluorescent pair bonded to a nucleic acid backbone chain, where the donor efficiently transfers energy to said acceptor for fluorescence of said acceptor; (2) each of the donor members of said labels absorbs at substantially the same wavelength all of the other donor members and each of the acceptor members of said labels emits at a different wavelength compared to any of the other acceptor members; and (3) each of said labels has substantially the same mobility in said separation resulting from varying the spacing of said donor-acceptor pair along said nucleic acid backbone, with the proviso that one of said labels can have the same fluorescer as the donor and the acceptor.
Claim 8
Dependent Claim
135 words
A kit comprising at least two fluorescent compounds for use as labels, each of said labels characterized by: (1) comprising an acceptor-donor fluorescent pair bonded to a nucleic acid backbone chain, where the donor efficiently transfers energy to said acceptor for fluorescence of said acceptor; (2) each of the donor members of said labels absorbs at substantially the same wavelength all of the other donor members and each of the acceptor members of said labels emits at a different wavelength compared to any of the other acceptor members; and (3) each of said labels has substantially the same mobility in said separation resulting from varying the spacing of said donor-acceptor pair along said nucleic acid backbone, with the proviso that one of said labels can have the same fluorescer as the donor and the acceptor.
Claim 9
Dependent Claim
28 words
A kit according to claim 6, wherein the acceptor and corresponding donor for all of said acceptor-donor fluorescent pairs are separated by not more than about 10 nucleotides.
Claim 10
Dependent Claim
50 words
A kit according to claim 6, wherein said donor absorb light in the wavelength range of 350-800 and said acceptors emit light in the wavelength range of 450-1000, wherein when said acceptor is the same as said donor, both absorption and emission are in the wavelength rnage of 350-800 nm.
Claim 11
Dependent Claim
17 words
A kit according to claim 8, wherein at least two acceptor--donor fluorescent pairs comprise a xanthene compound.
Claim 12
Dependent Claim
14 words
A kit according to claim 9, wherein said xanthene compound is fluorescein or rhodamine.
Claim 13
Dependent Claim
15 words
A kit according to claim 6, wherein one of said labels comprises fluorescein and rhodamine.
References Cited (Backward Citations)
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