Mammalian tub gene

Claim 2

An isolated nucleic acid molecule comprising: (a) a nucleic acid molecule encoding a polypeptide comprising the amino acid sequence shown in SEQ ID NO: 2; (b) a nucleic acid molecule encoding a polypeptide comprising the amino acid sequence shown in SEQ ID NO: 8; or (c) a nucleic acid molecule encoding a polypeptide comprising the amino acid sequence encoded by the nucleic acid insert of the clone contained in ATCC Deposit No. 69856, 97222, 69874, 69857, 69858 or 69859.

Claim 3

The isolated nucleic acid molecule of claim 1 wherein the nucleic acid molecule has the nucleotide sequence shown in SEQ ID NO: 1 or SEQ ID NO: 7.

Claim 4

The isolated nucleic acid molecule of claim 1 wherein the nucleic acid molecule encodes the amino acid sequence shown in SEQ ID NO:2 or SEQ ID NO:8.

Claim 5

An isolated nucleic acid molecule comprising: (a) a nucleic acid molecule encoding a polypeptide comprising amino acid residues 1-133 shown in SEQ ID NO: 2; (b) a nucleic acid molecule encoding a polypeptide comprising amino acid residues 190-505 shown in SEQ ID NO: 2; (c) a nucleic acid molecule encoding a polypeptide comprising amino acid residues 1-133 and 190-505 shown in SEQ ID NO: 2; (d) a nucleic acid molecule encoding a polypeptide comprising amino acid residues 1-133 shown in SEQ ID NO: 8; (e) a nucleic acid molecule encoding a polypeptide comprising amino acid residues 190-506 shown in SEQ ID NO: 8; or (f) a nucleic acid molecule encoding a polypeptide comprising amino acid residues 1-133 and 190-506 shown in SEQ ID NO: 8.

Claim 6

An isolated nucleic acid molecule comprising: (a) a nucleic acid molecule encoding a polypeptide comprising amino acid residues 1-133 shown in SEQ ID NO: 2; (b) a nucleic acid molecule encoding a polypeptide comprising amino acid residues 190-505 shown in SEQ ID NO: 2; (c) a nucleic acid molecule encoding a polypeptide comprising amino acid residues 1-133 and 190-505 shown in SEQ ID NO: 2; (d) a nucleic acid molecule encoding a polypeptide comprising amino acid residues 1-133 shown in SEQ ID NO: 8; (e) a nucleic acid molecule encoding a polypeptide comprising amino acid residues 190-506 shown in SEQ ID NO: 8; or (f) a nucleic acid molecule encoding a polypeptide comprising amino acid residues 1-133 and 190-506 shown in SEQ ID NO: 8.

Claim 7

The isolated nucleic acid molecule of claim 4 wherein the polypeptide encoded by the nucleic acid molecule comprises amino acid residue 133 shown in SEQ ID NO: 2 attached via a peptide bond to amino acid residue 190 shown in SEQ ID NO: 2 so that amino acid residues 1-133 and 190-505 shown in SEQ ID NO: 2 are contiguously present within the polypeptide.

Claim 8

The isolated nucleic acid molecule of claim 4 wherein the polypeptide encoded by the nucleic acid molecule comprises amino acid residue 133 shown in SEQ ID NO: 8 attached via a peptide bond to amino acid residue 190 shown in SEQ ID NO: 8 so that amino acid residues 1-133 and 190-506 shown in SEQ ID NO: 8 are contiguously present within the polypeptide.

Claim 9

The isolated nucleic acid molecule of claim 4 wherein the nucleic acid molecule comprises: (a) nucleotides 1-399 shown in SEQ ID NO: 1; (b) nucleotides 570-1515 shown in SEQ ID NO: 1; (c) nucleotides 1-399 and 570-1515 shown in SEQ ID NO: 1; (d) nucleotides 1-399 shown in SEQ ID NO: 7; (e) nucleotides 570-1515 shown in SEQ ID NO: 7; or (f) nucleotides 1-399 and 570-1515 shown in SEQ ID NO: 7.

Claim 10

The isolated nucleic acid molecule of claim 7 wherein nucleotide 399 shown in SEQ ID NO: 1 is attached to nucleotide 570 shown in SEQ ID NO: 1 via a phosphodiester bond so that nucleotides 1-399 and 570-1515 shown in SEQ ID NO: 1 are present contiguously.

Claim 11

The isolated nucleic acid molecule of claim 7 wherein nucleotide 399 shown in SEQ ID NO: 7 is attached to nucleotide 570 shown in SEQ ID NO: 7 via a phosphodiester bond so that nucleotides 1-399 and 570-1515 shown in SEQ ID NO: 7 are present contiguously.

Claim 12

An isolated nucleic acid molecule which hybridizes to the complement of the nucleic acid molecule of claim 1 or 4 and encodes a body weight modulating protein.

Claim 13

An isolated nucleic acid molecule which hybridizes under stringent conditions to the complement of the nucleic acid molecule of claim 1 or 4.

Claim 14

The isolated nucleic acid molecule of claim 10 wherein the nucleic acid molecule encodes a naturally occurring protein.

Claim 15

The isolated nucleic acid molecule of claim 11 wherein the nucleic acid molecule encodes a naturally occurring protein.

Claim 16

An isolated nucleic acid molecule encoding a mammalian tub protein.

Claim 17

An isolated nucleic acid molecule encoding a mammalian tub protein.

Claim 18

A nucleotide vector containing the nucleotide sequence of the nucleic acid molecule of claim 1, 4 or 14.

Claim 19

An expression vector containing the nucleotide sequence of the nucleic acid molecule of claim 1, 4 or 14 in operative association with a nucleotide regulatory sequence that controls expression of the nucleotide sequence in a host cell.

Claim 20

The expression vector of claim 16, wherein said regulatory element is selected from the group consisting of the cytomegalovirus hCMV immediate early gene, the early or late promoters of SV40 adenovirus, the lac system, the trp system, the TAC system, the TRC system, the major operator and promoter regions of phage A, the control regions of fd coat protein, the promoter for 3-phosphoglycerate kinase, the promoters of acid phosphatase, and the promoters of the yeast α-mating factors.

Claim 21

A genetically engineered host cell that contains the nucleotide sequence of the nucleic acid molecule of claim 1, 4 or 14.

Claim 22

A genetically engineered host cell that contains the nucleotide sequence of the nucleic acid molecule of claim 1, 4 or 14 in operative association with a regulatory sequence that controls expression of the nucleotide sequence in the host cell.

Claim 23

A nucleotide vector containing the nucleotide sequence of the nucleic acid molecule of claim 10.

Claim 24

An expression vector containing the nucleotide sequence of the nucleic acid molecule of claim 10 in operative association with a nucleotide regulatory sequence that controls expression of the nucleotide sequence in a host cell.

Claim 25

A genetically engineered host cell that contains the nucleotide sequence of the nucleic acid molecule of claim 10.

Claim 26

A genetically engineered host cell that contains the nucleotide sequence of the nucleic acid molecule of claim 10 in operative association with a regulatory sequence that controls expression of the nucleotide sequence in the host cell.

Claim 27

A nucleotide vector containing the nucleotide sequence of the nucleic acid molecule of claim 11.

Claim 28

An expression vector containing the nucleotide sequence of the nucleic acid molecule of claim 11 in operative association with a nucleotide regulatory sequence that controls expression of the nucleotide sequence in a host cell.

Claim 29

A genetically engineered host cell that contains the nucleotide sequence of the nucleic acid molecule of claim 11.

Claim 30

A genetically engineered host cell that contains the nucleotide sequence of the nucleic acid molecule of claim 11 in operative association with a regulatory sequence that controls expression of the nucleotide sequence in the host cell.

Claim 31

An isolated nucleic acid molecule having at least 15 contiguous nucleotides of the nucleic acid sequence depicted in SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12 or SEQ ID NO:13, wherein the nucleic acid molecule specifically hybridizes to a mammalian tub gene.

Claim 32

An isolated nucleic acid molecule having at least 15 contiguous nucleotides of the nucleic acid sequence depicted in SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12 or SEQ ID NO:13, wherein the nucleic acid molecule specifically hybridizes to a mammalian tub gene.

Claim 33

The isolated nucleic acid molecule of claim 28 wherein the nucleic acid sequence is intronic sequence.

Claim 34

An isolated nucleic acid molecule having the reverse complement of at least 15 contiguous nucleotides of the nucleic acid sequence depicted in SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12 or SEQ ID NO:13, wherein the nucleic acid molecule specifically hybridizes to a mammalian tub gene.

Claim 35

An isolated nucleic acid molecule having the reverse complement of at least 15 contiguous nucleotides of the nucleic acid sequence depicted in SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12 or SEQ ID NO:13, wherein the nucleic acid molecule specifically hybridizes to a mammalian tub gene.

Claim 36

The isolated nucleic acid molecule of claim 30 wherein the nucleic acid sequence depicted is intronic sequence.